3fd2

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{{Seed}}
 
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[[Image:3fd2.jpg|left|200px]]
 
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==Crystal structure of mMsoI/DNA complex with calcium==
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The line below this paragraph, containing "STRUCTURE_3fd2", creates the "Structure Box" on the page.
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<StructureSection load='3fd2' size='340' side='right'caption='[[3fd2]], [[Resolution|resolution]] 2.69&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[3fd2]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Monomastix_sp._OKE-1 Monomastix sp. OKE-1] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3FD2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3FD2 FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.69&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
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{{STRUCTURE_3fd2| PDB=3fd2 | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3fd2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3fd2 OCA], [https://pdbe.org/3fd2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3fd2 RCSB], [https://www.ebi.ac.uk/pdbsum/3fd2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3fd2 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/C0JWR6_MONSK C0JWR6_MONSK]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fd/3fd2_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3fd2 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Homing endonucleases (HEs) cut long DNA target sites with high specificity to initiate and target the lateral transfer of mobile introns or inteins. This high site specificity of HEs makes them attractive reagents for gene targeting to promote DNA modification or repair. We have generated several hundred catalytically active, monomerized versions of the well-characterized homodimeric I-CreI and I-MsoI LAGLIDADG family homing endonuclease (LHE) proteins. Representative monomerized I-CreI and I-MsoI proteins (collectively termed mCreIs or mMsoIs) were characterized in detail by using a combination of biochemical, biophysical and structural approaches. We also demonstrated that both mCreI and mMsoI proteins can promote cleavage-dependent recombination in human cells. The use of single chain LHEs should simplify gene modification and targeting by requiring the expression of a single small protein in cells, rather than the coordinate expression of two separate protein coding genes as is required when using engineered heterodimeric zinc finger or homing endonuclease proteins.
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===Crystal structure of mMsoI/DNA complex with calcium===
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Generation of single-chain LAGLIDADG homing endonucleases from native homodimeric precursor proteins.,Li H, Pellenz S, Ulge U, Stoddard BL, Monnat RJ Jr Nucleic Acids Res. 2009 Apr;37(5):1650-62. Epub 2009 Jan 19. PMID:19153140<ref>PMID:19153140</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 3fd2" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_19153140}}, adds the Publication Abstract to the page
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*[[Endonuclease 3D structures|Endonuclease 3D structures]]
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(as it appears on PubMed at http://www.pubmed.gov), where 19153140 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_19153140}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Large Structures]]
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3FD2 is a 3 chains structure of sequences from [http://en.wikipedia.org/wiki/Eukaryota Eukaryota]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3FD2 OCA].
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[[Category: Monomastix sp. OKE-1]]
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[[Category: Synthetic construct]]
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==Reference==
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[[Category: Li H]]
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<ref group="xtra">PMID:19153140</ref><references group="xtra"/>
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[[Category: Monnat RJ]]
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[[Category: Eukaryota]]
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[[Category: Li, H.]]
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[[Category: Monnat, R J.]]
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[[Category: Chloroplast]]
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[[Category: Hydrolase/dna complex]]
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[[Category: Plastid]]
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[[Category: Protein-dna complex]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jul 1 09:11:39 2009''
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Current revision

Crystal structure of mMsoI/DNA complex with calcium

PDB ID 3fd2

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Proteopedia Page Contributors and Editors (what is this?)

OCA

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