3f1s

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{{Seed}}
 
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[[Image:3f1s.jpg|left|200px]]
 
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==Crystal structure of Protein Z complexed with protein Z-dependent inhibitor==
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The line below this paragraph, containing "STRUCTURE_3f1s", creates the "Structure Box" on the page.
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<StructureSection load='3f1s' size='340' side='right'caption='[[3f1s]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[3f1s]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3F1S OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3F1S FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=FLC:CITRATE+ANION'>FLC</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
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{{STRUCTURE_3f1s| PDB=3f1s | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3f1s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3f1s OCA], [https://pdbe.org/3f1s PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3f1s RCSB], [https://www.ebi.ac.uk/pdbsum/3f1s PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3f1s ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/ZPI_HUMAN ZPI_HUMAN] Inhibits activity of the coagulation protease factor Xa in the presence of PROZ, calcium and phospholipids.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/f1/3f1s_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3f1s ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Protein Z (PZ) binds to PZ-dependent inhibitor (ZPI) and accelerates the inhibition of the coagulation protease, activated factor X (FXa), in the presence of phospholipids and Ca2+. A 2.3A resolution crystal structure of PZ complexed with ZPI shows that ZPI is a typical serine protease inhibitor and that PZ has a serine protease fold with distorted oxyanion hole and S1 pocket. The 2 molecules bind with fully complementary surfaces spanning over 2400A(2) and involving extensive ionic and hydrophobic interactions. ZPI has an unusual shutter region with a negatively charged residue buried within the hydrophobic core of the molecule. This unique Asp(213) is critical in maintaining the balanced metastability required for optimal protease inhibition, especially when PZ is bound, with its replacement with Asn resulting in increased thermal stability, but decreased efficiency of protease inhibition. The structure of ZPI shows negatively and positively charged surfaces on top of the molecule, in keeping with mutagenesis studies in this work indicating exosite interactions with FXa when it docks on top of ZPI. As modeled in this study, the gamma-carboxy-glutamic acid-containing domains of PZ and FXa enable them to bind to the same phospholipid surfaces on platelet and other membranes, with optimal proximity for the inhibition of FXa by the complexed ZPI.
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===Crystal structure of Protein Z complexed with protein Z-dependent inhibitor===
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Crystal structure of protein Z-dependent inhibitor complex shows how protein Z functions as a cofactor in the membrane inhibition of factor X.,Wei Z, Yan Y, Carrell RW, Zhou A Blood. 2009 Oct 22;114(17):3662-7. Epub 2009 Jun 15. PMID:19528533<ref>PMID:19528533</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 3f1s" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_19528533}}, adds the Publication Abstract to the page
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*[[Serpin 3D structures|Serpin 3D structures]]
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(as it appears on PubMed at http://www.pubmed.gov), where 19528533 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_19528533}}
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__TOC__
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</StructureSection>
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==About this Structure==
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3F1S is a 2 chains structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3F1S OCA].
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==Reference==
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<ref group="xtra">PMID:19528533</ref><references group="xtra"/>
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[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Zhou, A.]]
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[[Category: Large Structures]]
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[[Category: Alternative splicing]]
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[[Category: Zhou A]]
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[[Category: Blood coagulation]]
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[[Category: Calcium]]
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[[Category: Cleavage on pair of basic residue]]
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[[Category: Complex]]
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[[Category: Egf-like domain]]
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[[Category: Gamma-carboxyglutamic acid]]
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[[Category: Glycoprotein]]
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[[Category: Hydrolase inhibitor/hydrolase complex]]
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[[Category: Hydroxylation]]
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[[Category: Polymorphism]]
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[[Category: Protease]]
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[[Category: Protease inhibitor]]
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[[Category: Pz]]
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[[Category: Secreted]]
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[[Category: Serine protease homolog]]
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[[Category: Serine protease inhibitor]]
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[[Category: Serpin]]
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[[Category: Zpi]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jul 1 09:13:09 2009''
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Current revision

Crystal structure of Protein Z complexed with protein Z-dependent inhibitor

PDB ID 3f1s

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