3hd8

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{{Seed}}
 
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[[Image:3hd8.jpg|left|200px]]
 
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==Crystal structure of the Triticum aestivum xylanase inhibitor-IIA in complex with bacillus subtilis xylanase==
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The line below this paragraph, containing "STRUCTURE_3hd8", creates the "Structure Box" on the page.
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<StructureSection load='3hd8' size='340' side='right'caption='[[3hd8]], [[Resolution|resolution]] 2.39&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[3hd8]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis] and [https://en.wikipedia.org/wiki/Triticum_aestivum Triticum aestivum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3HD8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3HD8 FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.39&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3hd8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3hd8 OCA], [https://pdbe.org/3hd8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3hd8 RCSB], [https://www.ebi.ac.uk/pdbsum/3hd8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3hd8 ProSAT]</span></td></tr>
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{{STRUCTURE_3hd8| PDB=3hd8 | SCENE= }}
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/Q53IQ4_WHEAT Q53IQ4_WHEAT]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/hd/3hd8_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3hd8 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Triticum aestivum xylanase inhibitor (TAXI)-type inhibitors are active against microbial xylanases from glycoside hydrolase family 11, but the inhibition strength and the specificity towards different xylanases differ between TAXI isoforms. Mutational and biochemical analyses of TAXI-I, TAXI-IIA and Bacillus subtilis xylanase A showed that inhibition strength and specificity depend on the identity of only a few key residues of inhibitor and xylanase [Fierens K et al. (2005) FEBS J 272, 5872-5882; Raedschelders G et al. (2005) Biochem Biophys Res Commun335, 512-522; Sorensen JF &amp; Sibbesen O (2006) Protein Eng Des Sel 19, 205-210; Bourgois TM et al. (2007) J Biotechnol 130, 95-105]. Crystallographic analysis of the structures of TAXI-IA and TAXI-IIA in complex with glycoside hydrolase family 11 B. subtilis xylanase A now provides a substantial explanation for these observations and a detailed insight into the structural determinants for inhibition strength and specificity. Structures of the xylanaseinhibitor complexes show that inhibition is established by loop interactions with active-site residues and substrate-mimicking contacts in the binding subsites. The interaction of residues Leu292 of TAXI-IA and Pro294 of TAXI-IIA with the -2 glycon subsite of the xylanase is shown to be critical for both inhibition strength and specificity. Also, detailed analysis of the interaction interfaces of the complexes illustrates that the inhibition strength of TAXI is related to the presence of an aspartate or asparagine residue adjacent to the acid/base catalyst of the xylanase, and therefore to the pH optimum of the xylanase. The lower the pH optimum of the xylanase, the stronger will be the interaction between enzyme and inhibitor, and the stronger the resulting inhibition.
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===Crystal structure of the Triticum aestivum xylanase inhibitor-IIA in complex with bacillus subtilis xylanase===
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Identification of structural determinants for inhibition strength and specificity of wheat xylanase inhibitors TAXI-IA and TAXI-IIA.,Pollet A, Sansen S, Raedschelders G, Gebruers K, Rabijns A, Delcour JA, Courtin CM FEBS J. 2009 Jul;276(14):3916-27. Epub 2009 Jun 17. PMID:19769747<ref>PMID:19769747</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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==About this Structure==
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</div>
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3HD8 is a 4 chains structure of sequences from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis] and [http://en.wikipedia.org/wiki/Triticum_aestivum Triticum aestivum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3HD8 OCA].
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<div class="pdbe-citations 3hd8" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
[[Category: Bacillus subtilis]]
[[Category: Bacillus subtilis]]
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[[Category: Endo-1,4-beta-xylanase]]
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[[Category: Large Structures]]
[[Category: Triticum aestivum]]
[[Category: Triticum aestivum]]
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[[Category: Courtin, C M.]]
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[[Category: Courtin CM]]
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[[Category: Gebruers, K.]]
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[[Category: Gebruers K]]
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[[Category: Pollet, A.]]
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[[Category: Pollet A]]
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[[Category: Rabijns, A.]]
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[[Category: Rabijns A]]
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[[Category: Raedschelders, G.]]
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[[Category: Raedschelders G]]
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[[Category: Sansen, S.]]
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[[Category: Sansen S]]
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[[Category: Beta-jelly roll]]
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[[Category: Glycosidase]]
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[[Category: Hydrolase]]
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[[Category: Hydrolase inhibitor/hydrolase complex]]
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[[Category: Protein-protein complex]]
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[[Category: Two beta-barrel domain]]
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[[Category: X-ray crystallography]]
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[[Category: Xylan degradation]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jul 1 09:41:10 2009''
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Current revision

Crystal structure of the Triticum aestivum xylanase inhibitor-IIA in complex with bacillus subtilis xylanase

PDB ID 3hd8

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