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3e8u
From Proteopedia
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| - | {{Seed}} | ||
| - | [[Image:3e8u.jpg|left|200px]] | ||
| - | + | ==Crystal structure and thermodynamic analysis of diagnostic Fab 106.3 complexed with BNP 5-13 (C10A) reveal basis of selective molecular recognition== | |
| - | + | <StructureSection load='3e8u' size='340' side='right'caption='[[3e8u]], [[Resolution|resolution]] 2.10Å' scene=''> | |
| - | You may | + | == Structural highlights == |
| - | + | <table><tr><td colspan='2'>[[3e8u]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3E8U OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3E8U FirstGlance]. <br> | |
| - | + | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3e8u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3e8u OCA], [https://pdbe.org/3e8u PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3e8u RCSB], [https://www.ebi.ac.uk/pdbsum/3e8u PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3e8u ProSAT]</span></td></tr> | |
| - | -- | + | </table> |
| - | + | == Evolutionary Conservation == | |
| + | [[Image:Consurf_key_small.gif|200px|right]] | ||
| + | Check<jmol> | ||
| + | <jmolCheckbox> | ||
| + | <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/e8/3e8u_consurf.spt"</scriptWhenChecked> | ||
| + | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | ||
| + | <text>to colour the structure by Evolutionary Conservation</text> | ||
| + | </jmolCheckbox> | ||
| + | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3e8u ConSurf]. | ||
| + | <div style="clear:both"></div> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | B-type natriuretic peptide (BNP) is a naturally secreted regulatory hormone that influences blood pressure and vascular water retention in human physiology. The plasma BNP concentration is a clinically recognized biomarker for various cardiovascular diseases. Quantitative detection of BNP can be achieved in immunoassays using the high-affinity monoclonal IgG1 antibody 106.3, which binds an epitope spanning residues 5-13 of the mature bioactive peptide. To understand the structural basis of this molecular recognition, we crystallized the Fab fragment complexed with the peptide epitope and determined the three-dimensional structure by X-ray diffraction to 2.1 A resolution. The structure reveals the detailed interactions that five of the complementarity-determining regions make with the partially folded peptide. Thermodynamic measurements using fluorescence spectroscopy suggest that the interaction is enthalpy driven, with an overall change in free energy of binding, DeltaG = -54 kJ/mol, at room temperature. The parameters are interpreted on the basis of the structural information. The kinetics of binding suggest a diffusion-limited mechanism, whereby the peptide easily adopts a bound conformation upon interaction with the antibody. Moreover, comparative analysis with alanine-scanning results of the epitope explains the basis of selectivity for BNP over other related natriuretic peptides. | ||
| - | + | Crystal structure and thermodynamic analysis of diagnostic mAb 106.3 complexed with BNP 5-13 (C10A).,Longenecker KL, Ruan Q, Fry EH, Saldana SC, Brophy SE, Richardson PL, Tetin SY Proteins. 2009 Aug 15;76(3):536-47. PMID:19274732<ref>PMID:19274732</ref> | |
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 3e8u" style="background-color:#fffaf0;"></div> | ||
| - | + | ==See Also== | |
| - | + | *[[Antibody 3D structures|Antibody 3D structures]] | |
| - | + | *[[3D structures of non-human antibody|3D structures of non-human antibody]] | |
| - | + | == References == | |
| - | + | <references/> | |
| - | + | __TOC__ | |
| - | == | + | </StructureSection> |
| - | + | [[Category: Large Structures]] | |
| - | + | [[Category: Lk3 transgenic mice]] | |
| - | == | + | [[Category: Brophy, S E]] |
| - | < | + | [[Category: Fry, E H]] |
| - | [[Category: | + | [[Category: Longenecker, K L]] |
| - | [[Category: Brophy, S E | + | [[Category: Richardson, P L]] |
| - | [[Category: Fry, E H | + | [[Category: Ruan, Q]] |
| - | [[Category: Longenecker, K L | + | [[Category: Saldana, S S]] |
| - | [[Category: Richardson, P L | + | [[Category: Tetin, S Y]] |
| - | [[Category: Ruan, Q | + | |
| - | [[Category: Saldana, S S | + | |
| - | [[Category: Tetin, S Y | + | |
[[Category: Fab 106 3]] | [[Category: Fab 106 3]] | ||
[[Category: Igg1]] | [[Category: Igg1]] | ||
[[Category: Immune system]] | [[Category: Immune system]] | ||
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| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jul 8 09:33:01 2009'' | ||
Current revision
Crystal structure and thermodynamic analysis of diagnostic Fab 106.3 complexed with BNP 5-13 (C10A) reveal basis of selective molecular recognition
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