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1fd5

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BINDING OF A MACROCYCLIC BISACRIDINE AND AMETANTRONE TO CGTACG INVOLVES SIMILAR UNUSUAL INTERCALATION PLATFORMS (BISACRIDINE COMPLEX)

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Retrieved from "http://52.214.119.220/wiki/index.php/1fd5"

Categories: Large Structures | Gao Y-G | Robinson H | Wang AH-J | Yang X-L

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-[[Image:1fd5.gif|left|200px]]<br /><applet load="1fd5" size="450" color="white" frame="true" align="right" spinBox="true"
-caption="1fd5, resolution 1.1&Aring;" />
-'''BINDING OF A MACROCYCLIC BISACRIDINE AND AMETANTRONE TO CGTACG INVOLVES SIMILAR UNUSUAL INTERCALATION PLATFORMS (BISACRIDINE COMPLEX)'''<br />
-==Overview==
+==BINDING OF A MACROCYCLIC BISACRIDINE AND AMETANTRONE TO CGTACG INVOLVES SIMILAR UNUSUAL INTERCALATION PLATFORMS (BISACRIDINE COMPLEX)==
-The binding of a macrocyclic bisacridine and an antitumor intercalator, ametantrone to DNA has been studied. We carried out X-ray diffraction, analyses of the complexes between both intercalators and CGTACG. We have, determined the crystal structure, by the multiple-wavelength anomalous, diffraction (MAD) method, of bisacridine complexed with CGTA[br(5)C]G at, 1.8 A resolution. The refined native crystal structure at 1.1 A resolution, (space group C222, a = 29.58 A, b = 54.04 A, c = 40.22 A, and R-factor =, 0.163) revealed that only one acridine of the bisacridine drug binds at, the C5pG6 step of the DNA, with the other acridine plus both linkers, completely disordered. Surprisingly, both terminal G.C base pairs are, unraveled. The C1 nucleotide is disordered, and the G2 base is bridged to, its own phosphate P2 through a hydrated Co(2+) ion. G12 is swung toward, the minor groove with its base stacked over the backbone. The C7, nucleotide is flipped away from the duplex part and base paired to a, 2-fold symmetry-related G6. The central four base pairs adopt the B-DNA, conformation. An unusual intercalator platform is formed by bringing four, complexes together (involving the 222 symmetry) such that the intercalator, cavity is flanked by two sets of G x C base pairs (i.e., C5 x G8 and G6 x, C7) on each side, joined together by G6 x G8 tertiary base pairing, interactions. In the bisacridine-CGTACG complex, the intercalation, platform is intercalated with two acridines, whereas in the, ametantrone-CGTACG complex, only one ametantrone is bound. NMR titration, of the bisacridine to AACGATCGTT suggests that the bisacridine prefers to, bridge more than one DNA duplex by intercalating each acridine to, different duplexes. The results may be relevant in understanding binding, of certain intercalators to DNA structure associated with the quadruplet, helix and Holliday junction.
+<StructureSection load='1fd5' size='340' side='right'caption='[[1fd5]], [[Resolution|resolution]] 1.10&Aring;' scene=''>
+== Structural highlights ==
-==About this Structure==
+<table><tr><td colspan='2'>[[1fd5]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FD5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1FD5 FirstGlance]. <br>
-FD5 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with CO, MG and B9A as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1FD5 OCA].
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.1&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=B9A:MACROCYCLIC-BIS-9-AMINO-ACRIDINE'>B9A</scene>, <scene name='pdbligand=CO:COBALT+(II)+ION'>CO</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
-==Reference==
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1fd5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1fd5 OCA], [https://pdbe.org/1fd5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1fd5 RCSB], [https://www.ebi.ac.uk/pdbsum/1fd5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1fd5 ProSAT]</span></td></tr>
-Binding of a macrocyclic bisacridine and ametantrone to CGTACG involves similar unusual intercalation platforms., Yang XL, Robinson H, Gao YG, Wang AH, Biochemistry. 2000 Sep 12;39(36):10950-7. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10998231 10998231]
+</table>
-[[Category: Protein complex]]
+__TOC__
-[[Category: Gao, Y.G.]]
+</StructureSection>
-[[Category: Robinson, H.]]
+[[Category: Large Structures]]
-[[Category: Wang, A.H.J.]]
+[[Category: Gao Y-G]]
-[[Category: Yang, X.L.]]
+[[Category: Robinson H]]
-[[Category: B9A]]
+[[Category: Wang AH-J]]
-[[Category: CO]]
+[[Category: Yang X-L]]
-[[Category: MG]]
-[[Category: anticancer drug]]
-[[Category: drug-dna complex]]
-[[Category: high resolution crystal structure]]
-[[Category: intercalation platform]]
-[[Category: mad]]
-[[Category: metal ions]]
-[[Category: tertiary base pairs]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sat Nov 24 23:40:28 2007''

1fd5

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BINDING OF A MACROCYCLIC BISACRIDINE AND AMETANTRONE TO CGTACG INVOLVES SIMILAR UNUSUAL INTERCALATION PLATFORMS (BISACRIDINE COMPLEX)

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