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1s1l

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(New page: 200px<br /><applet load="1s1l" size="450" color="white" frame="true" align="right" spinBox="true" caption="1s1l, resolution 2.20&Aring;" /> '''Influence of Groove ...)
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[[Image:1s1l.gif|left|200px]]<br /><applet load="1s1l" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1s1l, resolution 2.20&Aring;" />
 
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'''Influence of Groove Interactions on the Formation of DNA Holliday Junctions'''<br />
 
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==Overview==
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==Influence of Groove Interactions on the Formation of DNA Holliday Junctions==
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The inosine-containing sequence d(CCIGTACm(5)CGG) is shown to crystallize, as a four-stranded DNA junction. This structure is nearly identical to the, antiparallel junction formed by the parent d(CCGGTACm(5)()CGG) sequence, [Vargason, J. M., and Ho, P. S. (2002) J. Biol. Chem. 277, 21041-21049] in, terms of its conformational geometry, and inter- and intramolecular, interactions within the DNA and between the DNA and solvent, even though, the 2-amino group in the minor groove of the important G(3).m(5)C(8) base, pair of the junction core trinucleotide (italicized) has been removed. In, contrast, the analogous 2,6-diaminopurine sequence d(CCDGTACTGG), crystallizes as resolved duplex DNAs, just like its parent sequence, d(CCAGTACTGG) [Hays, F. A., Vargason, J. M., and Ho, P. S. (2003), Biochemistry 42, 9586-9597]. These results demonstrate that it is not the, presence or absence of the 2-amino group in the minor groove of the, R(3).Y(8) base pair that specifies whether a sequence forms a junction, but the positions of the extracyclic amino and keto groups in the major, groove. Finally, the study shows that the arms of the junction can, accommodate perturbations to the B-DNA conformation of the stacked duplex, arms associated with the loss of the 2-amino substituent, and that two, hydrogen bonding interactions from the C(7) and Y(8) pyrimidine, nucleotides to phosphate oxygens of the junction crossover specify the, geometry of the Holliday junction.
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<StructureSection load='1s1l' size='340' side='right'caption='[[1s1l]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1s1l]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1S1L OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1S1L FirstGlance]. <br>
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1S1L is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1S1L OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=5CM:5-METHYL-2-DEOXY-CYTIDINE-5-MONOPHOSPHATE'>5CM</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1s1l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1s1l OCA], [https://pdbe.org/1s1l PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1s1l RCSB], [https://www.ebi.ac.uk/pdbsum/1s1l PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1s1l ProSAT]</span></td></tr>
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Influence of minor groove substituents on the structure of DNA Holliday junctions., Hays FA, Jones ZJ, Ho PS, Biochemistry. 2004 Aug 3;43(30):9813-22. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15274635 15274635]
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</table>
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[[Category: Protein complex]]
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__TOC__
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[[Category: Hays, F.A.]]
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</StructureSection>
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[[Category: Ho, P.S.]]
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[[Category: Large Structures]]
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[[Category: Jones, Z.J.]]
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[[Category: Hays FA]]
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[[Category: dna four-way junction]]
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[[Category: Ho PS]]
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[[Category: holliday junction]]
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[[Category: Jones ZJ]]
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[[Category: inosine]]
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[[Category: major groove]]
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[[Category: minor groove]]
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[[Category: x-ray diffraction]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sat Nov 24 23:41:56 2007''
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Influence of Groove Interactions on the Formation of DNA Holliday Junctions

PDB ID 1s1l

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