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1s23

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(New page: 200px<br /><applet load="1s23" size="450" color="white" frame="true" align="right" spinBox="true" caption="1s23, resolution 1.60&Aring;" /> '''Crystal Structure An...)
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[[Image:1s23.gif|left|200px]]<br /><applet load="1s23" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1s23, resolution 1.60&Aring;" />
 
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'''Crystal Structure Analysis of the B-DNA Decamer CGCAATTGCG'''<br />
 
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==Overview==
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==Crystal Structure Analysis of the B-DNA Decamer CGCAATTGCG==
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The deoxyoligonucleotide d(CGCAATTGCG) has previously been crystallized in, four different space groups. The crystals diffract to moderate resolution, (2.3-2.9 A). Here, a fifth crystal form that diffracts to higher, resolution (1.6 A) is presented which was obtained thanks to the use of, Co2+ and cryogenic temperatures. The availability of five different, crystal structures allows a thorough analysis of the conformational, variability of this DNA sequence. It is concluded that the central hexamer, sequence CAATTG has a practically constant conformation under all, conditions, whilst the terminal base pairs at both ends vary considerably, as a result of differing interactions in the crystals. The new crystal, structure presented here is stabilized by guanine-Co2+-guanine, interactions and the formation of C1+ -G8.C3 triplexes between, neighbouring duplexes. As a result of the higher resolution of the crystal, structure, a more regular structure was obtained and a clear definition of, the spine of hydration was observed which was not visible in the four, previous structures.
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<StructureSection load='1s23' size='340' side='right'caption='[[1s23]], [[Resolution|resolution]] 1.60&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1s23]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1S23 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1S23 FirstGlance]. <br>
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1S23 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with CO as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1S23 OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.6&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CO:COBALT+(II)+ION'>CO</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1s23 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1s23 OCA], [https://pdbe.org/1s23 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1s23 RCSB], [https://www.ebi.ac.uk/pdbsum/1s23 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1s23 ProSAT]</span></td></tr>
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DNA variability in five crystal structures of d(CGCAATTGCG)., Valls N, Wright G, Steiner RA, Murshudov GN, Subirana JA, Acta Crystallogr D Biol Crystallogr. 2004 Apr;60(Pt 4):680-5. Epub 2004, Mar 23. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15039556 15039556]
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</table>
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[[Category: Protein complex]]
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__TOC__
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[[Category: Murshudov, G.N.]]
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</StructureSection>
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[[Category: Steiner, R.A.]]
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[[Category: Large Structures]]
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[[Category: Subirana, J.A.]]
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[[Category: Murshudov GN]]
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[[Category: Valls, N.]]
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[[Category: Steiner RA]]
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[[Category: Wright, G.]]
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[[Category: Subirana JA]]
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[[Category: CO]]
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[[Category: Valls N]]
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[[Category: cobalt]]
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[[Category: Wright G]]
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[[Category: deoxyoligonucleotide]]
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[[Category: variability]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sat Nov 24 23:43:39 2007''
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Current revision

Crystal Structure Analysis of the B-DNA Decamer CGCAATTGCG

PDB ID 1s23

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