1fg0

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(New page: 200px<br /><applet load="1fg0" size="450" color="white" frame="true" align="right" spinBox="true" caption="1fg0, resolution 3.00&Aring;" /> '''LARGE RIBOSOMAL SUBU...)
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[[Image:1fg0.gif|left|200px]]<br /><applet load="1fg0" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1fg0, resolution 3.00&Aring;" />
 
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'''LARGE RIBOSOMAL SUBUNIT COMPLEXED WITH A 13 BP MINIHELIX-PUROMYCIN COMPOUND'''<br />
 
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==Overview==
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==LARGE RIBOSOMAL SUBUNIT COMPLEXED WITH A 13 BP MINIHELIX-PUROMYCIN COMPOUND==
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Using the atomic structures of the large ribosomal subunit from Haloarcula, marismortui and its complexes with two substrate analogs, we establish, that the ribosome is a ribozyme and address the catalytic properties of, its all-RNA active site. Both substrate analogs are contacted exclusively, by conserved ribosomal RNA (rRNA) residues from domain V of 23S rRNA;, there are no protein side-chain atoms closer than about 18 angstroms to, the peptide bond being synthesized. The mechanism of peptide bond, synthesis appears to resemble the reverse of the acylation step in serine, proteases, with the base of A2486 (A2451 in Escherichia coli) playing the, same general base role as histidine-57 in chymotrypsin. The unusual pK(a), (where K(a) is the acid dissociation constant) required for A2486 to, perform this function may derive in part from its hydrogen bonding to, G2482 (G2447 in E. coli), which also interacts with a buried phosphate, that could stabilize unusual tautomers of these two bases. The polypeptide, exit tunnel is largely formed by RNA but has significant contributions, from proteins L4, L22, and L39e, and its exit is encircled by proteins, L19, L22, L23, L24, L29, and L31e.
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<StructureSection load='1fg0' size='340' side='right'caption='[[1fg0]], [[Resolution|resolution]] 3.00&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1fg0]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Haloarcula_marismortui Haloarcula marismortui] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FG0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1FG0 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PPU:PUROMYCIN-5-MONOPHOSPHATE'>PPU</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1fg0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1fg0 OCA], [https://pdbe.org/1fg0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1fg0 RCSB], [https://www.ebi.ac.uk/pdbsum/1fg0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1fg0 ProSAT]</span></td></tr>
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</table>
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==About this Structure==
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==See Also==
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1FG0 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Haloarcula_marismortui Haloarcula marismortui]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1FG0 OCA].
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*[[Large Ribosomal Subunit of Haloarcula|Large Ribosomal Subunit of Haloarcula]]
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*[[Ribosome|Ribosome]]
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==Reference==
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__TOC__
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The structural basis of ribosome activity in peptide bond synthesis., Nissen P, Hansen J, Ban N, Moore PB, Steitz TA, Science. 2000 Aug 11;289(5481):920-30. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10937990 10937990]
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</StructureSection>
[[Category: Haloarcula marismortui]]
[[Category: Haloarcula marismortui]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Ban, N.]]
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[[Category: Synthetic construct]]
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[[Category: Hansen, J.]]
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[[Category: Ban N]]
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[[Category: Moore, P.B.]]
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[[Category: Hansen J]]
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[[Category: Nissen, P.]]
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[[Category: Moore PB]]
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[[Category: Steitz, T.A.]]
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[[Category: Nissen P]]
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[[Category: a-site]]
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[[Category: Steitz TA]]
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[[Category: ribosome assembly]]
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[[Category: ribozyme]]
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[[Category: rna-rna]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sat Nov 24 23:46:11 2007''
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Current revision

LARGE RIBOSOMAL SUBUNIT COMPLEXED WITH A 13 BP MINIHELIX-PUROMYCIN COMPOUND

PDB ID 1fg0

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