1ntf

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(New page: 200px<br /><applet load="1ntf" size="450" color="white" frame="true" align="right" spinBox="true" caption="1ntf, resolution 1.80&Aring;" /> '''Crystal Structure of...)
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[[Image:1ntf.gif|left|200px]]<br /><applet load="1ntf" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1ntf, resolution 1.80&Aring;" />
 
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'''Crystal Structure of Cimex Nitrophorin'''<br />
 
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==Overview==
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==Crystal Structure of Cimex Nitrophorin==
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Certain bloodsucking insects deliver nitric oxide (NO) while feeding, to, induce vasodilation and inhibit blood coagulation. We have expressed, characterized, and determined the crystal structure of the Cimex, lectularius (bedbug) nitrophorin, the protein responsible for NO storage, and delivery, to understand how the insect successfully handles this, reactive molecule. Surprisingly, NO binds not only to the ferric, nitrophorin heme, but it can also be stored as an S-nitroso (SNO), conjugate of the proximal heme cysteine (Cys-60) when present at higher, concentrations. EPR- and UV-visible spectroscopies, and a crystallographic, structure determination to 1.75-A resolution, reveal SNO formation to, proceed with reduction of the heme iron, yielding an Fe-NO complex., Stopped-flow kinetic measurements indicate that an ordered reaction, mechanism takes place: initial NO binding occurs at the ferric heme and is, followed by heme reduction, Cys-60 release from the heme iron, and SNO, formation. Release of NO occurs through a reversal of these steps. These, data provide, to our knowledge, the first view of reversible, metal-assisted SNO formation in a protein and suggest a mechanism for its, role in NO release from ferrous heme. This mechanism and Cimex nitrophorin, structure are completely unlike those of the nitrophorins from Rhodnius, prolixus, where NO protection is provided by a large conformational change, that buries the heme nitrosyl complex, highlighting the remarkable, evolution of proteins that assist insects in bloodfeeding.
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<StructureSection load='1ntf' size='340' side='right'caption='[[1ntf]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1ntf]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Cimex_lectularius Cimex lectularius]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NTF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1NTF FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ntf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ntf OCA], [https://pdbe.org/1ntf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ntf RCSB], [https://www.ebi.ac.uk/pdbsum/1ntf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ntf ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/NP_CIMLE NP_CIMLE] Heme-based protein that delivers nitric oxide gas (NO) to the victim while feeding, resulting in vasodilation (Probable). In place of heme, the heme-binding cysteine can also reversibly bind NO when it is present in high concentrations (PubMed:15637157).<ref>PMID:15637157</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/nt/1ntf_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ntf ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1NTF is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Cimex_lectularius Cimex lectularius] with HEM as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1NTF OCA].
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*[[Nitrophorin|Nitrophorin]]
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== References ==
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==Reference==
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<references/>
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Heme-assisted S-nitrosation of a proximal thiolate in a nitric oxide transport protein., Weichsel A, Maes EM, Andersen JF, Valenzuela JG, Shokhireva TKh, Walker FA, Montfort WR, Proc Natl Acad Sci U S A. 2005 Jan 18;102(3):594-9. Epub 2005 Jan 6. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15637157 15637157]
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__TOC__
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</StructureSection>
[[Category: Cimex lectularius]]
[[Category: Cimex lectularius]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Andersen, J.F.]]
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[[Category: Andersen JF]]
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[[Category: Maes, E.M.]]
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[[Category: Maes EM]]
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[[Category: Montfort, W.R.]]
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[[Category: Montfort WR]]
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[[Category: Valenzuela, J.G.]]
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[[Category: Valenzuela JG]]
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[[Category: Walker, F.A.]]
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[[Category: Walker FA]]
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[[Category: Weichsel, A.]]
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[[Category: Weichsel A]]
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[[Category: HEM]]
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[[Category: beta sandwich]]
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[[Category: ferric heme protein]]
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[[Category: no carrier]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sat Nov 24 23:47:01 2007''
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Current revision

Crystal Structure of Cimex Nitrophorin

PDB ID 1ntf

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