3h8k

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{{Seed}}
 
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[[Image:3h8k.jpg|left|200px]]
 
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==Crystal structure of Ube2g2 complxed with the G2BR domain of gp78 at 1.8-A resolution==
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The line below this paragraph, containing "STRUCTURE_3h8k", creates the "Structure Box" on the page.
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<StructureSection load='3h8k' size='340' side='right'caption='[[3h8k]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[3h8k]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3H8K OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3H8K FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3h8k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3h8k OCA], [https://pdbe.org/3h8k PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3h8k RCSB], [https://www.ebi.ac.uk/pdbsum/3h8k PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3h8k ProSAT]</span></td></tr>
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{{STRUCTURE_3h8k| PDB=3h8k | SCENE= }}
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/UB2G2_HUMAN UB2G2_HUMAN] Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-48'-linked polyubiquitination. Involved in endoplasmic reticulum-associated degradation (ERAD).<ref>PMID:20061386</ref> <ref>PMID:22607976</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/h8/3h8k_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3h8k ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The activity of RING finger ubiquitin ligases (E3) is dependent on their ability to facilitate transfer of ubiquitin from ubiquitin-conjugating enzymes (E2) to substrates. The G2BR domain within the E3 gp78 binds selectively and with high affinity to the E2 Ube2g2. Through structural and functional analyses, we determine that this occurs on a region of Ube2g2 distinct from binding sites for ubiquitin-activating enzyme (E1) and RING fingers. Binding to the G2BR results in conformational changes in Ube2g2 that affect ubiquitin loading. The Ube2g2:G2BR interaction also causes an approximately 50-fold increase in affinity between the E2 and RING finger. This results in markedly increased ubiquitylation by Ube2g2 and the gp78 RING finger. The significance of this G2BR effect is underscored by enhanced ubiquitylation observed when Ube2g2 is paired with other RING finger E3s. These findings uncover a mechanism whereby allosteric effects on an E2 enhance E2-RING finger interactions and, consequently, ubiquitylation.
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===Crystal structure of Ube2g2 complxed with the G2BR domain of gp78 at 1.8-A resolution===
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Allosteric activation of E2-RING finger-mediated ubiquitylation by a structurally defined specific E2-binding region of gp78.,Das R, Mariano J, Tsai YC, Kalathur RC, Kostova Z, Li J, Tarasov SG, McFeeters RL, Altieri AS, Ji X, Byrd RA, Weissman AM Mol Cell. 2009 Jun 26;34(6):674-85. PMID:19560420<ref>PMID:19560420</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 3h8k" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_19560420}}, adds the Publication Abstract to the page
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*[[3D structures of ubiquitin conjugating enzyme|3D structures of ubiquitin conjugating enzyme]]
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(as it appears on PubMed at http://www.pubmed.gov), where 19560420 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_19560420}}
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__TOC__
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</StructureSection>
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==About this Structure==
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3H8K is a 2 chains structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3H8K OCA].
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==Reference==
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<ref group="xtra">PMID:19560420</ref><references group="xtra"/>
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[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Ubiquitin--protein ligase]]
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[[Category: Large Structures]]
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[[Category: Byrd, A R.]]
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[[Category: Byrd RA]]
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[[Category: Das, R.]]
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[[Category: Das R]]
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[[Category: Ji, X.]]
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[[Category: Ji X]]
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[[Category: Kalathur, R C.]]
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[[Category: Kalathur RC]]
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[[Category: Li, J.]]
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[[Category: Li J]]
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[[Category: All alpha]]
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[[Category: Alpha beta]]
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[[Category: Alternative splicing]]
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[[Category: Endoplasmic reticulum]]
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[[Category: Ligase]]
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[[Category: Membrane]]
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[[Category: Metal-binding]]
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[[Category: Phosphoprotein]]
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[[Category: Polymorphism]]
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[[Category: Receptor]]
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[[Category: Transmembrane]]
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[[Category: Ubl conjugation pathway]]
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[[Category: Zinc]]
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[[Category: Zinc-finger]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jul 15 08:44:07 2009''
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Current revision

Crystal structure of Ube2g2 complxed with the G2BR domain of gp78 at 1.8-A resolution

PDB ID 3h8k

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