3gzt

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (09:12, 30 October 2024) (edit) (undo)
 
(10 intermediate revisions not shown.)
Line 1: Line 1:
-
{{Seed}}
 
-
[[Image:3gzt.jpg|left|200px]]
 
-
<!--
+
==VP7 recoated rotavirus DLP==
-
The line below this paragraph, containing "STRUCTURE_3gzt", creates the "Structure Box" on the page.
+
<SX load='3gzt' size='340' side='right' viewer='molstar' caption='[[3gzt]], [[Resolution|resolution]] 3.80&Aring;' scene=''>
-
You may change the PDB parameter (which sets the PDB file loaded into the applet)
+
== Structural highlights ==
-
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+
<table><tr><td colspan='2'>[[3gzt]] is a 13 chain structure with sequence from [https://en.wikipedia.org/wiki/Simian_rotavirus_A_strain_RRV Simian rotavirus A strain RRV]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3GZT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3GZT FirstGlance]. <br>
-
or leave the SCENE parameter empty for the default display.
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.8&#8491;</td></tr>
-
-->
+
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
-
{{STRUCTURE_3gzt| PDB=3gzt | SCENE= }}
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3gzt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3gzt OCA], [https://pdbe.org/3gzt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3gzt RCSB], [https://www.ebi.ac.uk/pdbsum/3gzt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3gzt ProSAT]</span></td></tr>
 +
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/VP7_ROTRH VP7_ROTRH] Outer capsid protein involved in attachment and possibly entry into the host epithelial cell. It is subsequently lost, together with VP4, following virus entry into the host cell. The outer layer contains 780 copies of VP7, grouped as 260 trimers. Rotavirus attachment and entry into the host cell probably involves multiple sequential contacts between the outer capsid proteins VP4 and VP7, and the cell receptors. In integrin-dependent strains, VP7 seems to essentially target the integrin heterodimers ITGAX/ITGB2 and ITGA5/ITGB3 at a postbinding stage, once the initial attachment by VP4 has been achieved (By similarity).
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/gz/3gzt_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3gzt ConSurf].
 +
<div style="clear:both"></div>
 +
<div style="background-color:#fffaf0;">
 +
== Publication Abstract from PubMed ==
 +
Rotaviruses, major causes of childhood gastroenteritis, are nonenveloped, icosahedral particles with double-strand RNA genomes. By the use of electron cryomicroscopy and single-particle reconstruction, we have visualized a rotavirus particle comprising the inner capsid coated with the trimeric outer-layer protein, VP7, at a resolution (4 A) comparable with that of X-ray crystallography. We have traced the VP7 polypeptide chain, including parts not seen in its X-ray crystal structure. The 3 well-ordered, 30-residue, N-terminal "arms" of each VP7 trimer grip the underlying trimer of VP6, an inner-capsid protein. Structural differences between free and particle-bound VP7 and between free and VP7-coated inner capsids may regulate mRNA transcription and release. The Ca(2+)-stabilized VP7 intratrimer contact region, which presents important neutralizing epitopes, is unaltered upon capsid binding.
-
===VP7 recoated rotavirus DLP===
+
Molecular interactions in rotavirus assembly and uncoating seen by high-resolution cryo-EM.,Chen JZ, Settembre EC, Aoki ST, Zhang X, Bellamy AR, Dormitzer PR, Harrison SC, Grigorieff N Proc Natl Acad Sci U S A. 2009 Jun 30;106(26):10644-8. Epub 2009 Jun 1. PMID:19487668<ref>PMID:19487668</ref>
 +
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 +
</div>
 +
<div class="pdbe-citations 3gzt" style="background-color:#fffaf0;"></div>
-
<!--
+
==See Also==
-
The line below this paragraph, {{ABSTRACT_PUBMED_19487668}}, adds the Publication Abstract to the page
+
*[[Virus coat proteins 3D structures|Virus coat proteins 3D structures]]
-
(as it appears on PubMed at http://www.pubmed.gov), where 19487668 is the PubMed ID number.
+
== References ==
-
-->
+
<references/>
-
{{ABSTRACT_PUBMED_19487668}}
+
__TOC__
-
 
+
</SX>
-
==About this Structure==
+
[[Category: Large Structures]]
-
3GZT is a 13 chains structure of sequences from [http://en.wikipedia.org/wiki/Rhesus_rotavirus Rhesus rotavirus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3GZT OCA].
+
[[Category: Simian rotavirus A strain RRV]]
-
 
+
[[Category: Chen JZ]]
-
==Reference==
+
[[Category: Grigorieff N]]
-
<ref group="xtra">PMID:19487668</ref><references group="xtra"/>
+
[[Category: Harrison SC]]
-
[[Category: Rhesus rotavirus]]
+
[[Category: Settembre EC]]
-
[[Category: Chen, J Z.]]
+
-
[[Category: Grigorieff, N.]]
+
-
[[Category: Harrison, S C.]]
+
-
[[Category: Settembre, E C.]]
+
-
[[Category: 7rp]]
+
-
[[Category: Capsid protein]]
+
-
[[Category: Disulfide bond]]
+
-
[[Category: Dlp]]
+
-
[[Category: Endoplasmic reticulum]]
+
-
[[Category: Glycoprotein]]
+
-
[[Category: Icosahedral virus]]
+
-
[[Category: Membrane]]
+
-
[[Category: Rotavirus]]
+
-
[[Category: Transmembrane]]
+
-
[[Category: Virion]]
+
-
[[Category: Vp2]]
+
-
[[Category: Vp6]]
+
-
[[Category: Vp7]]
+
-
 
+
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jul 15 08:44:16 2009''
+

Current revision

VP7 recoated rotavirus DLP

3gzt, resolution 3.80Å

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3gzt"
Personal tools