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3gua

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{{Seed}}
 
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[[Image:3gua.jpg|left|200px]]
 
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==Sulfates bound in the vestibule of AChBP==
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The line below this paragraph, containing "STRUCTURE_3gua", creates the "Structure Box" on the page.
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<StructureSection load='3gua' size='340' side='right'caption='[[3gua]], [[Resolution|resolution]] 3.10&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[3gua]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Aplysia_californica Aplysia californica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3GUA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3GUA FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.1&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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{{STRUCTURE_3gua| PDB=3gua | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3gua FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3gua OCA], [https://pdbe.org/3gua PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3gua RCSB], [https://www.ebi.ac.uk/pdbsum/3gua PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3gua ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/Q8WSF8_APLCA Q8WSF8_APLCA]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/gu/3gua_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3gua ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Neurotransmitter binding to Cys-loop receptors promotes a prodigious transmembrane flux of several million ions/s, but to date, structural determinants of ion flux have been identified flanking the membrane-spanning region. Using x-ray crystallography, sequence analysis, and single-channel recording, we identified a novel determinant of ion conductance near the point of entry of permeant ions. Co-crystallization of acetylcholine-binding protein with sulfate anions revealed coordination of SO4(2-) with a ring of lysines at a position equivalent to 24 A above the lipid membrane in homologous Cys-loop receptors. Analysis of multiple sequence alignments revealed that residues equivalent to the ring of lysines are negatively charged in cation-selective receptors but are positively charged in anion-selective receptors. Charge reversal of side chains at homologous positions in the nicotinic receptor from the motor end plate decreases unitary conductance up to 80%. Selectivity filters stemming from transmembrane alpha-helices have similar pore diameters and compositions of amino acids. These findings establish that when the channel opens under a physiological electrochemical gradient, permeant ions are initially stabilized within the extracellular vestibule of Cys-loop receptors, and this stabilization is a major determinant of ion conductance.
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===Sulfates bound in the vestibule of AChBP===
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An ion selectivity filter in the extracellular domain of Cys-loop receptors reveals determinants for ion conductance.,Hansen SB, Wang HL, Taylor P, Sine SM J Biol Chem. 2008 Dec 26;283(52):36066-70. Epub 2008 Oct 21. PMID:18940802<ref>PMID:18940802</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 3gua" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_18940802}}, adds the Publication Abstract to the page
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*[[Acetylcholine binding protein 3D structures|Acetylcholine binding protein 3D structures]]
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(as it appears on PubMed at http://www.pubmed.gov), where 18940802 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_18940802}}
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__TOC__
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</StructureSection>
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==About this Structure==
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3GUA is a 10 chains structure of sequences from [http://en.wikipedia.org/wiki/Aplysia_californica Aplysia californica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3GUA OCA].
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==Reference==
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<ref group="xtra">PMID:18940802</ref><references group="xtra"/>
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[[Category: Aplysia californica]]
[[Category: Aplysia californica]]
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[[Category: Hansen, S B.]]
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[[Category: Large Structures]]
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[[Category: Taylor, P.]]
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[[Category: Hansen SB]]
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[[Category: Choline-binding protein]]
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[[Category: Taylor P]]
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[[Category: Ion channel]]
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[[Category: Ion filter]]
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[[Category: Ion selectivity]]
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[[Category: Receptor]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jul 15 08:44:52 2009''
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Current revision

Sulfates bound in the vestibule of AChBP

PDB ID 3gua

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