2w39

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{{Seed}}
 
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[[Image:2w39.jpg|left|200px]]
 
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==Glc(beta-1-3)Glc disaccharide in -1 and -2 sites of Laminarinase 16A from Phanerochaete chrysosporium==
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The line below this paragraph, containing "STRUCTURE_2w39", creates the "Structure Box" on the page.
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<StructureSection load='2w39' size='340' side='right'caption='[[2w39]], [[Resolution|resolution]] 1.10&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2w39]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Phanerodontia_chrysosporium Phanerodontia chrysosporium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2W39 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2W39 FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.1&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=LGC:(3S,4R,5R,6S)-3,4,5-TRIHYDROXY-6-(HYDROXYMETHYL)TETRAHYDRO-2H-PYRAN-2-ONE'>LGC</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=PRD_900024:beta-laminaribiose'>PRD_900024</scene></td></tr>
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{{STRUCTURE_2w39| PDB=2w39 | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2w39 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2w39 OCA], [https://pdbe.org/2w39 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2w39 RCSB], [https://www.ebi.ac.uk/pdbsum/2w39 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2w39 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/Q874E3_PHACH Q874E3_PHACH]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/w3/2w39_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2w39 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The 1,3(4)-beta-D-glucanases of glycoside hydrolase family 16 provide useful examples of versatile yet specific protein-carbohydrate interactions. In the present study, we report the X-ray structures of the 1,3(4)-beta-D-glucanase Phanerochaete chrysosporium Laminarinase 16A in complex with beta-glucan products from laminarin (1.6 A) and lichenin (1.1 A) hydrolysis. The G6G3G3G glucan, in complex with the enzyme, showed a beta-1,6 branch in the acceptor site. The G4G3G ligand-protein complex showed that there was no room for a beta-1,6 branch in the -1 or -2 subsites; furthermore, the distorted residue in the -1 subsite and the glucose in the -2 subsite required a beta-1,3 bond between them. These are the first X-ray crystal structures of any 1,3(4)-beta-D-glucanase in complex with glucan products. They provide details of both substrate and product binding in support of earlier enzymatic evidence.
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===GLC(BETA-1-3)GLC DISACCHARIDE IN -1 AND -2 SITES OF LAMINARINASE 16A FROM PHANEROCHAETE CHRYSOSPORIUM===
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X-ray crystal structures of Phanerochaete chrysosporium Laminarinase 16A in complex with products from lichenin and laminarin hydrolysis.,Vasur J, Kawai R, Andersson E, Igarashi K, Sandgren M, Samejima M, Stahlberg J FEBS J. 2009 Jul;276(14):3858-69. Epub 2009 Jun 17. PMID:19769746<ref>PMID:19769746</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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==About this Structure==
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</div>
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2W39 is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Phanerochaete_chrysosporium Phanerochaete chrysosporium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2W39 OCA].
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<div class="pdbe-citations 2w39" style="background-color:#fffaf0;"></div>
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[[Category: Phanerochaete chrysosporium]]
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== References ==
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[[Category: Andersson, E.]]
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<references/>
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[[Category: Igarashi, K.]]
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__TOC__
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[[Category: Kawai, R.]]
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</StructureSection>
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[[Category: Samejima, M.]]
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[[Category: Large Structures]]
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[[Category: Sandgren, M.]]
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[[Category: Phanerodontia chrysosporium]]
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[[Category: Stahlberg, J.]]
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[[Category: Andersson E]]
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[[Category: Vasur, J.]]
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[[Category: Igarashi K]]
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[[Category: Basidiomycete]]
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[[Category: Kawai R]]
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[[Category: Beta sandwich]]
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[[Category: Samejima M]]
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[[Category: Beta-glucan]]
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[[Category: Sandgren M]]
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[[Category: Beta-glucanase]]
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[[Category: Stahlberg J]]
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[[Category: Extracellular]]
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[[Category: Vasur J]]
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[[Category: Family 16]]
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[[Category: Gh16]]
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[[Category: Gh7]]
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[[Category: Glycosyl hydrolase]]
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[[Category: Hydrolase]]
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[[Category: Lam16a]]
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[[Category: Laminarin]]
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[[Category: Laminarinase]]
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[[Category: Phanerochaete chrysosporium]]
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[[Category: Pichea pastori]]
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[[Category: White rot fungus]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jul 22 20:30:30 2009''
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Current revision

Glc(beta-1-3)Glc disaccharide in -1 and -2 sites of Laminarinase 16A from Phanerochaete chrysosporium

PDB ID 2w39

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