1jr9

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(New page: 200px<br /><applet load="1jr9" size="450" color="white" frame="true" align="right" spinBox="true" caption="1jr9, resolution 2.8&Aring;" /> '''Crystal Structure of ...)
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[[Image:1jr9.jpg|left|200px]]<br /><applet load="1jr9" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1jr9, resolution 2.8&Aring;" />
 
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'''Crystal Structure of manganese superoxide dismutases from Bacillus halodenitrificans'''<br />
 
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==Overview==
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==Crystal Structure of manganese superoxide dismutases from Bacillus halodenitrificans==
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Manganese superoxide dismutase (GP-MnSOD), a component of the so-called, 'green protein' (green protein complex) from the facultative anaerobic, halodenitrifier Bacillus halodenitrificans, has been crystallized using, the hanging-drop vapor diffusion method. Crystals have unit-cell, parameters a=b=93.4 A, c=65.0 A, and belong to the space group P4(3)2(1)2., Preliminary analysis indicates there is one monomer in each asymmetric, unit. The structural information from this enzyme will enrich our, knowledge on its high catalytic activity and its possible role in green, protein complex.
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<StructureSection load='1jr9' size='340' side='right'caption='[[1jr9]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1jr9]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Virgibacillus_halodenitrificans Virgibacillus halodenitrificans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JR9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1JR9 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.8&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1jr9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1jr9 OCA], [https://pdbe.org/1jr9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1jr9 RCSB], [https://www.ebi.ac.uk/pdbsum/1jr9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1jr9 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/SODM_VIRHA SODM_VIRHA] Destroys superoxide anion radicals which are normally produced within the cells and which are toxic to biological systems. Active only in homodimeric state.<ref>PMID:12457847</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/jr/1jr9_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1jr9 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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A so-called "green protein" has been purified from a moderate halophilic eubacterium, Bacillus halodenitrificans (ATCC 49067), under anaerobic conditions. The protein, which might play an important role in denitrification, dissociates mainly into two components after exposure to air: a manganese superoxide dismutase (GP-MnSOD) and a nucleoside diphosphate kinase. As a first step in elucidating the overall structure of the green protein and the role of each component, the 2.8-A resolution crystal structure of GP-MnSOD was determined. Compared with other manganese dismutases, GP-MnSOD shows two significant characteristics. The first is that the entrance to its substrate channel has an additional basic residue-Lys38. The second is that its surface is decorated with an excess of acidic over basic residues. All these structural features may be related to GP-MnSOD's high catalytic activity and its endurance against the special cytoplasm of B. halodenitrificans. The structure of GP-MnSOD provides the basis for recognizing its possible role and assembly state in the green protein.
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==About this Structure==
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Three-dimensional structure of manganese superoxide dismutase from Bacillus halodenitrificans, a component of the so-called "green protein".,Liao J, Liu MY, Chang T, Li M, Le Gall J, Gui LL, Zhang JP, Jiang T, Liang DC, Chang WR J Struct Biol. 2002 Sep;139(3):171-80. PMID:12457847<ref>PMID:12457847</ref>
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1JR9 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Virgibacillus_halodenitrificans Virgibacillus halodenitrificans] with MN and ZN as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Superoxide_dismutase Superoxide dismutase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.15.1.1 1.15.1.1] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1JR9 OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Crystallization and preliminary crystallographic analysis of manganese superoxide dismutase from Bacillus halodenitrificans., Liao J, Li M, Liu MY, Chang T, Le Gall J, Gui LL, Zhang JP, Liang DC, Chang WR, Biochem Biophys Res Commun. 2002 May 31;294(1):60-2. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12054740 12054740]
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</div>
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[[Category: Single protein]]
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<div class="pdbe-citations 1jr9" style="background-color:#fffaf0;"></div>
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[[Category: Superoxide dismutase]]
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[[Category: Virgibacillus halodenitrificans]]
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[[Category: Chang, T.]]
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[[Category: Chang, W.R.]]
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[[Category: Gui, L.L.]]
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[[Category: Jiang, T.]]
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[[Category: LeGall, J.]]
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[[Category: Li, M.]]
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[[Category: Liang, D.C.]]
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[[Category: Liao, J.]]
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[[Category: Liu, M.Y.]]
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[[Category: Zhang, J.P.]]
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[[Category: MN]]
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[[Category: ZN]]
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[[Category: manganese superoxide dismutase]]
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[[Category: oxidoreductase]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sun Nov 25 00:05:36 2007''
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==See Also==
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*[[Superoxide dismutase 3D structures|Superoxide dismutase 3D structures]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
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[[Category: Virgibacillus halodenitrificans]]
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[[Category: Chang T]]
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[[Category: Chang WR]]
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[[Category: Gui LL]]
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[[Category: Jiang T]]
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[[Category: LeGall J]]
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[[Category: Li M]]
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[[Category: Liang DC]]
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[[Category: Liao J]]
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[[Category: Liu MY]]
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[[Category: Zhang JP]]

Current revision

Crystal Structure of manganese superoxide dismutases from Bacillus halodenitrificans

PDB ID 1jr9

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