1s9s

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(New page: 200px<br /><applet load="1s9s" size="450" color="white" frame="true" align="right" spinBox="true" caption="1s9s" /> '''SOLUTION STRUCTURE OF MLV PSI SITE'''<br /> ...)
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[[Image:1s9s.gif|left|200px]]<br /><applet load="1s9s" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1s9s" />
 
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'''SOLUTION STRUCTURE OF MLV PSI SITE'''<br />
 
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==Overview==
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==SOLUTION STRUCTURE OF MLV PSI SITE==
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The full length, positive-strand genome of the Moloney Murine Leukemia, Virus contains a "core encapsidation signal" that is essential for, efficient genome packaging during virus assembly. We have determined the, structure of a 101-nucleotide RNA that contains this signal (called mPsi), using a novel isotope-edited NMR approach. The method is robust and should, be generally applicable to larger RNAs. mPsi folds into three stem loops, two of which (SL-C and SL-D) co-stack to form an extended helix. The third, stem loop (SL-B) is connected to SL-C by a flexible, four-nucleotide, linker. The structure contains five mismatched base-pairs, an unusual C.CG, base-triple platform, and a novel "A-minor K-turn," in which unpaired, adenosine bases A340 and A341 of a GGAA bulge pack in the minor groove of, a proximal stem, and a bulged distal uridine (U319) forms a hydrogen bond, with the phosphodiester of A341. Phylogenetic analyses indicate that these, essential structural elements are conserved among the murine C-type, retroviruses.
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<StructureSection load='1s9s' size='340' side='right'caption='[[1s9s]]' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1s9s]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Moloney_murine_leukemia_virus Moloney murine leukemia virus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1S9S OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1S9S FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1s9s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1s9s OCA], [https://pdbe.org/1s9s PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1s9s RCSB], [https://www.ebi.ac.uk/pdbsum/1s9s PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1s9s ProSAT]</span></td></tr>
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</table>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The full length, positive-strand genome of the Moloney Murine Leukemia Virus contains a "core encapsidation signal" that is essential for efficient genome packaging during virus assembly. We have determined the structure of a 101-nucleotide RNA that contains this signal (called mPsi) using a novel isotope-edited NMR approach. The method is robust and should be generally applicable to larger RNAs. mPsi folds into three stem loops, two of which (SL-C and SL-D) co-stack to form an extended helix. The third stem loop (SL-B) is connected to SL-C by a flexible, four-nucleotide linker. The structure contains five mismatched base-pairs, an unusual C.CG base-triple platform, and a novel "A-minor K-turn," in which unpaired adenosine bases A340 and A341 of a GGAA bulge pack in the minor groove of a proximal stem, and a bulged distal uridine (U319) forms a hydrogen bond with the phosphodiester of A341. Phylogenetic analyses indicate that these essential structural elements are conserved among the murine C-type retroviruses.
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==About this Structure==
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NMR structure of the 101-nucleotide core encapsidation signal of the Moloney murine leukemia virus.,D'Souza V, Dey A, Habib D, Summers MF J Mol Biol. 2004 Mar 19;337(2):427-42. PMID:15003457<ref>PMID:15003457</ref>
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1S9S is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1S9S OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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NMR structure of the 101-nucleotide core encapsidation signal of the Moloney murine leukemia virus., D'Souza V, Dey A, Habib D, Summers MF, J Mol Biol. 2004 Mar 19;337(2):427-42. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15003457 15003457]
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</div>
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[[Category: Protein complex]]
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<div class="pdbe-citations 1s9s" style="background-color:#fffaf0;"></div>
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[[Category: Dey, A.]]
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== References ==
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[[Category: Habib, D.]]
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<references/>
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[[Category: Souza, V.D.]]
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__TOC__
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[[Category: Summers, M.F.]]
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</StructureSection>
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[[Category: a-c mismatch]]
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[[Category: Large Structures]]
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[[Category: a-minor k-turn]]
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[[Category: Moloney murine leukemia virus]]
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[[Category: bulge]]
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[[Category: D'Souza V]]
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[[Category: g-a mismatch]]
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[[Category: Dey A]]
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[[Category: g-u mismatch]]
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[[Category: Habib D]]
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[[Category: mlv]]
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[[Category: Summers MF]]
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[[Category: stem loop]]
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[[Category: u-u mismatch]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sun Nov 25 00:08:34 2007''
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Current revision

SOLUTION STRUCTURE OF MLV PSI SITE

PDB ID 1s9s

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