1sb6

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(New page: 200px<br /><applet load="1sb6" size="450" color="white" frame="true" align="right" spinBox="true" caption="1sb6" /> '''Solution structure of a cyanobacterial coppe...)
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[[Image:1sb6.jpg|left|200px]]<br /><applet load="1sb6" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1sb6" />
 
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'''Solution structure of a cyanobacterial copper metallochaperone, ScAtx1'''<br />
 
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==Overview==
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==Solution structure of a cyanobacterial copper metallochaperone, ScAtx1==
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The Atx1 copper metallochaperone from Synechocystis PCC 6803, ScAtx1, interacts with two P(1)-type copper ATPases to supply copper proteins, within intracellular compartments, avoiding ATPases for other metals en, route. Here we report NMR-derived solution structures for ScAtx1. The, monomeric apo form has a betaalphabetabetaalpha fold with backbone motions, largely restricted to loop 1 containing Cys-12 and Cys-15. The tumbling, rate of Cu(I)ScAtx1 (0.1-0.8 mm) implies dimers. Experimental restraints, are satisfied by symmetrical dimers with Cys-12 or His-61, but not Cys-15, invading the copper site of the opposing subunit. A full sequence of, copper ligands from the cell surface to thylakoid compartments is, proposed, considering in vitro homodimer liganding to mimic in vivo, liganding in ScAtx1-ATPase heterodimers. A monomeric high resolution, structure for Cu(I)ScAtx1, with Cys-12, Cys-15, and His-61 as ligands, is, calculated without violations despite the rotational correlation time., (2)J(NH) couplings in the imidazole ring of His-61 establish coordination, of N(epsilon2) to copper. His-61 is analogous to Lys-65 in eukaryotic, metallochaperones, stabilizing Cu(I)S(2) complexes but by binding Cu(I), rather than compensating charge. Cys-Cys-His ligand sets are an emergent, theme in some copper metallochaperones, although not in related Atx1, CopZ, or Hah1. Surface charge (Glu-13) close to the metal-binding site of, ScAtx1 is likely to support interaction with complementary surfaces of, copper-transporting ATPases (PacS-Arg-11 and CtaA-Lys-14) but to, discourage interaction with zinc ATPase ZiaA and so inhibit aberrant, formation of copper-ZiaA complexes.
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<StructureSection load='1sb6' size='340' side='right'caption='[[1sb6]]' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1sb6]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Synechocystis_sp._PCC_6803 Synechocystis sp. PCC 6803]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SB6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1SB6 FirstGlance]. <br>
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1SB6 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Synechocystis_sp. Synechocystis sp.]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1SB6 OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1sb6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1sb6 OCA], [https://pdbe.org/1sb6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1sb6 RCSB], [https://www.ebi.ac.uk/pdbsum/1sb6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1sb6 ProSAT]</span></td></tr>
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==Reference==
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</table>
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Solution structures of a cyanobacterial metallochaperone: insight into an atypical copper-binding motif., Banci L, Bertini I, Ciofi-Baffoni S, Su XC, Borrelly GP, Robinson NJ, J Biol Chem. 2004 Jun 25;279(26):27502-10. Epub 2004 Apr 8. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15075318 15075318]
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== Function ==
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[[Category: Single protein]]
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[https://www.uniprot.org/uniprot/P73213_SYNY3 P73213_SYNY3]
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[[Category: Synechocystis sp.]]
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== Evolutionary Conservation ==
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[[Category: Banci, L.]]
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[[Image:Consurf_key_small.gif|200px|right]]
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[[Category: Bertini, I.]]
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Check<jmol>
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[[Category: Borrelly, G.P.]]
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<jmolCheckbox>
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[[Category: Ciofi-Baffoni, S.]]
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/sb/1sb6_consurf.spt"</scriptWhenChecked>
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[[Category: Robinson, N.J.]]
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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[[Category: SPINE, Structural.Proteomics.in.Europe.]]
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<text>to colour the structure by Evolutionary Conservation</text>
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[[Category: Su, X.C.]]
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</jmolCheckbox>
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[[Category: copper chaperone]]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1sb6 ConSurf].
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[[Category: new metal binding motif]]
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<div style="clear:both"></div>
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[[Category: nmr]]
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__TOC__
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[[Category: spine]]
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</StructureSection>
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[[Category: structural genomics]]
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[[Category: Large Structures]]
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[[Category: structural proteomics in europe]]
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[[Category: Synechocystis sp. PCC 6803]]
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[[Category: structure]]
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[[Category: Banci L]]
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[[Category: Bertini I]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sun Nov 25 00:12:30 2007''
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[[Category: Borrelly GP]]
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[[Category: Ciofi-Baffoni S]]
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[[Category: Robinson NJ]]
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[[Category: Su XC]]

Current revision

Solution structure of a cyanobacterial copper metallochaperone, ScAtx1

PDB ID 1sb6

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