2wk8

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{{Seed}}
 
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[[Image:2wk8.png|left|200px]]
 
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==Structure of holo form of Vibrio cholerae CqsA==
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The line below this paragraph, containing "STRUCTURE_2wk8", creates the "Structure Box" on the page.
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<StructureSection load='2wk8' size='340' side='right'caption='[[2wk8]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2wk8]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Vibrio_cholerae_O1_biovar_El_Tor_str._N16961 Vibrio cholerae O1 biovar El Tor str. N16961]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WK8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2WK8 FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PLP:PYRIDOXAL-5-PHOSPHATE'>PLP</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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{{STRUCTURE_2wk8| PDB=2wk8 | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2wk8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wk8 OCA], [https://pdbe.org/2wk8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2wk8 RCSB], [https://www.ebi.ac.uk/pdbsum/2wk8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2wk8 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/CQSA_VIBCH CQSA_VIBCH] Required for the synthesis of the quorum-sensing autoinducer CAI-1 ((S)-3-hydroxytridecan-4-one) which probably functions as an intragenus signal.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/wk/2wk8_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2wk8 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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CqsA is an enzyme involved in the biosynthesis of cholerae autoinducer-1 (CAI-1), the major Vibrio cholerae autoinducer engaged in quorum sensing. The amino acid sequence of CqsA suggests that it belongs to the family of alpha-oxoamine synthases that catalyse the condensation of an amino acid to an acyl-CoA substrate. Here we present the apo- and PLP-bound crystal structures of CqsA and confirm that it shares structural homology with the dimeric alpha-oxoamine synthases, including a conserved PLP-binding site. The chemical structure of CAI-1 suggests that decanoyl-CoA may be one substrate of CqsA and that another substrate may be l-threonine or l-2-aminobutyric acid. A crystal structure of CqsA at 1.9-A resolution obtained in the presence of PLP and l-threonine reveals an external aldimine that has lost the l-threonine side chain. Similarly, a 1.9-A-resolution crystal structure of CqsA in the presence of PLP, l-threonine, and decanoyl-CoA shows a trapped external aldimine intermediate, suggesting that the condensation and decarboxylation steps have occurred, again with loss of the l-threonine side chain. It is suggested that this side-chain loss, an observation supported by mass spectrometry, is due to a retro-aldol reaction. Although no structural data have been obtained on CqsA using l-2-aminobutyric acid and decanoyl-CoA as substrates, mass spectrometry confirms the expected product of the enzyme reaction. It is proposed that a region of structure that is disordered in the apo structure is involved in the release of product. While not confirming if CqsA alone is able to synthesize CAI-1, these results suggest possible synthetic routes.
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===STRUCTURE OF HOLO FORM OF VIBRIO CHOLERAE CQSA===
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Insights into the biosynthesis of the Vibrio cholerae major autoinducer CAI-1 from the crystal structure of the PLP-dependent enzyme CqsA.,Jahan N, Potter JA, Sheikh MA, Botting CH, Shirran SL, Westwood NJ, Taylor GL J Mol Biol. 2009 Sep 25;392(3):763-73. Epub 2009 Jul 22. PMID:19631226<ref>PMID:19631226</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_19631226}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 2wk8" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 19631226 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_19631226}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Large Structures]]
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2WK8 is a 2 chains structure of sequences from [http://en.wikipedia.org/wiki/Vibrio_cholerae_o1_biovar_el_tor Vibrio cholerae o1 biovar el tor]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WK8 OCA].
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[[Category: Vibrio cholerae O1 biovar El Tor str. N16961]]
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[[Category: Botting CH]]
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==Reference==
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[[Category: Jahan N]]
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<ref group="xtra">PMID:19631226</ref><references group="xtra"/>
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[[Category: Potter JA]]
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[[Category: Vibrio cholerae o1 biovar el tor]]
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[[Category: Sheikh MA]]
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[[Category: Botting, C H.]]
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[[Category: Shirran SL]]
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[[Category: Jahan, N.]]
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[[Category: Taylor GL]]
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[[Category: Potter, J A.]]
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[[Category: Westwood NJ]]
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[[Category: Sheikh, M A.]]
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[[Category: Shirran, S L.]]
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[[Category: Taylor, G L.]]
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[[Category: Westwood, N J.]]
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[[Category: Acyltransferase]]
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[[Category: Aminotransferase]]
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[[Category: Autoinducer]]
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[[Category: Cai-1]]
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[[Category: Cqsa]]
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[[Category: Oxoamine-synthase]]
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[[Category: Pyridoxal phosphate]]
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[[Category: Quorum-sensing]]
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[[Category: Transferase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Aug 5 08:49:24 2009''
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Current revision

Structure of holo form of Vibrio cholerae CqsA

PDB ID 2wk8

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