1sdm

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(New page: 200px<br /><applet load="1sdm" size="450" color="white" frame="true" align="right" spinBox="true" caption="1sdm, resolution 2.30&Aring;" /> '''Crystal structure of...)
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[[Image:1sdm.jpg|left|200px]]<br /><applet load="1sdm" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1sdm, resolution 2.30&Aring;" />
 
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'''Crystal structure of kinesin-like calmodulin binding protein'''<br />
 
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==Overview==
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==Crystal structure of kinesin-like calmodulin binding protein==
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Kinesins orchestrate cell division by controlling placement of, chromosomes. Kinesins must be precisely regulated or else cell division, fails. Calcium, a universal second messenger in eukaryotes, and calmodulin, regulate some kinesins by causing the motor to dissociate from its, biological track, the microtubule. Our focus was the mechanism of calcium, regulation of kinesin at atomic resolution. Here we report the crystal, structure of kinesin-like calmodulin-binding protein (KCBP) from potato, which was resolved to 2.3 A. The structure reveals three subdomains of the, regulatory machinery located at the C terminus extension of the kinesin, motor. Calmodulin that is activated by Ca2+ ions binds to an alpha-helix, positioned on the microtubule-binding face of kinesin. A negatively, charged segment following this helix competes with microtubules. A mimic, of the conventional kinesin neck, connecting the calmodulin-binding helix, to the KCBP motor core, links the regulatory machine to the kinesin, catalytic cycle. Together with biochemical data, the crystal structure, suggests that Ca(2+)-calmodulin inhibits the binding of KCBP to, microtubules by blocking the microtubule-binding sites on KCBP.
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<StructureSection load='1sdm' size='340' side='right'caption='[[1sdm]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1sdm]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Solanum_tuberosum Solanum tuberosum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SDM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1SDM FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1sdm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1sdm OCA], [https://pdbe.org/1sdm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1sdm RCSB], [https://www.ebi.ac.uk/pdbsum/1sdm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1sdm ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/Q41460_SOLTU Q41460_SOLTU]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/sd/1sdm_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1sdm ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Kinesins orchestrate cell division by controlling placement of chromosomes. Kinesins must be precisely regulated or else cell division fails. Calcium, a universal second messenger in eukaryotes, and calmodulin regulate some kinesins by causing the motor to dissociate from its biological track, the microtubule. Our focus was the mechanism of calcium regulation of kinesin at atomic resolution. Here we report the crystal structure of kinesin-like calmodulin-binding protein (KCBP) from potato, which was resolved to 2.3 A. The structure reveals three subdomains of the regulatory machinery located at the C terminus extension of the kinesin motor. Calmodulin that is activated by Ca2+ ions binds to an alpha-helix positioned on the microtubule-binding face of kinesin. A negatively charged segment following this helix competes with microtubules. A mimic of the conventional kinesin neck, connecting the calmodulin-binding helix to the KCBP motor core, links the regulatory machine to the kinesin catalytic cycle. Together with biochemical data, the crystal structure suggests that Ca(2+)-calmodulin inhibits the binding of KCBP to microtubules by blocking the microtubule-binding sites on KCBP.
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==About this Structure==
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Crystal structure of kinesin regulated by Ca(2+)-calmodulin.,Vinogradova MV, Reddy VS, Reddy AS, Sablin EP, Fletterick RJ J Biol Chem. 2004 May 28;279(22):23504-9. Epub 2004 Feb 26. PMID:14988396<ref>PMID:14988396</ref>
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1SDM is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Solanum_tuberosum Solanum tuberosum] with MG and ADP as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1SDM OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Crystal structure of kinesin regulated by Ca(2+)-calmodulin., Vinogradova MV, Reddy VS, Reddy AS, Sablin EP, Fletterick RJ, J Biol Chem. 2004 May 28;279(22):23504-9. Epub 2004 Feb 26. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=14988396 14988396]
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</div>
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[[Category: Single protein]]
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<div class="pdbe-citations 1sdm" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Solanum tuberosum]]
[[Category: Solanum tuberosum]]
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[[Category: Fletterick, R.J.]]
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[[Category: Fletterick RJ]]
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[[Category: Reddy, A.S.]]
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[[Category: Reddy AS]]
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[[Category: Reddy, V.S.]]
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[[Category: Reddy VS]]
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[[Category: Sablin, E.P.]]
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[[Category: Sablin EP]]
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[[Category: Vinogradova, M.V.]]
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[[Category: Vinogradova MV]]
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[[Category: ADP]]
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[[Category: MG]]
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[[Category: ca2+/calmodulin regulated]]
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[[Category: kinesin]]
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[[Category: minus-end directed]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sun Nov 25 00:18:39 2007''
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Current revision

Crystal structure of kinesin-like calmodulin binding protein

PDB ID 1sdm

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