2rq4

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[[Image:2rq4.jpg|left|200px]]
 
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==Refinement of RNA binding domain 3 in CUG triplet repeat RNA-binding protein 1==
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The line below this paragraph, containing "STRUCTURE_2rq4", creates the "Structure Box" on the page.
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<StructureSection load='2rq4' size='340' side='right'caption='[[2rq4]]' scene=''>
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2rq4]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RQ4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2RQ4 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2rq4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2rq4 OCA], [https://pdbe.org/2rq4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2rq4 RCSB], [https://www.ebi.ac.uk/pdbsum/2rq4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2rq4 ProSAT]</span></td></tr>
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{{STRUCTURE_2rq4| PDB=2rq4 | SCENE= }}
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/CELF1_HUMAN CELF1_HUMAN] RNA-binding protein implicated in the regulation of several post-transcriptional events. Involved in pre-mRNA alternative splicing, mRNA translation and stability. Mediates exon inclusion and/or exclusion in pre-mRNA that are subject to tissue-specific and developmentally regulated alternative splicing. Specifically activates exon 5 inclusion of cardiac isoforms of TNNT2 during heart remodeling at the juvenile to adult transition. Acts as both an activator and repressor of a pair of coregulated exons: promotes inclusion of the smooth muscle (SM) exon but exclusion of the non-muscle (NM) exon in actinin pre-mRNAs. Activates SM exon 5 inclusion by antagonizing the repressive effect of PTB. Promotes exclusion of exon 11 of the INSR pre-mRNA. Inhibits, together with HNRNPH1, insulin receptor (IR) pre-mRNA exon 11 inclusion in myoblast. Increases translation and controls the choice of translation initiation codon of CEBPB mRNA. Increases mRNA translation of CEBPB in aging liver (By similarity). Increases translation of CDKN1A mRNA by antagonizing the repressive effect of CALR3. Mediates rapid cytoplasmic mRNA deadenylation. Recruits the deadenylase PARN to the poly(A) tail of EDEN-containing mRNAs to promote their deadenylation. Required for completion of spermatogenesis (By similarity). Binds to (CUG)n triplet repeats in the 3'-UTR of transcripts such as DMPK and to Bruno response elements (BREs). Binds to muscle-specific splicing enhancer (MSE) intronic sites flanking the alternative exon 5 of TNNT2 pre-mRNA. Binds to AU-rich sequences (AREs or EDEN-like) localized in the 3'-UTR of JUN and FOS mRNAs. Binds to the IR RNA. Binds to the 5'-region of CDKN1A and CEBPB mRNAs. Binds with the 5'-region of CEBPB mRNA in aging liver.<ref>PMID:10536163</ref> <ref>PMID:11124939</ref> <ref>PMID:11158314</ref> <ref>PMID:12799066</ref> <ref>PMID:12649496</ref> <ref>PMID:14726956</ref> <ref>PMID:16946708</ref> <ref>PMID:16601207</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/rq/2rq4_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2rq4 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The CUG-binding protein 1 (CUG-BP1) is a member of the CUG-BP1 and ETR-like factors (CELF) family or the Bruno-like family and is involved in the control of splicing, translation and mRNA degradation. Several target RNA sequences of CUG-BP1 have been predicted, such as the CUG triplet repeat, the GU-rich sequences and the AU-rich element of nuclear pre-mRNAs and/or cytoplasmic mRNA. CUG-BP1 has three RNA-recognition motifs (RRMs), among which the third RRM (RRM3) can bind to the target RNAs on its own. In this study, we solved the solution structure of the CUG-BP1 RRM3 by hetero-nuclear NMR spectroscopy. The CUG-BP1 RRM3 exhibited a noncanonical RRM fold, with the four-stranded beta-sheet surface tightly associated with the N-terminal extension. Furthermore, we determined the solution structure of the CUG-BP1 RRM3 in the complex with (UG)(3) RNA, and discovered that the UGU trinucleotide is specifically recognized through extensive stacking interactions and hydrogen bonds within the pocket formed by the beta-sheet surface and the N-terminal extension. This study revealed the unique mechanism that enables the CUG-BP1 RRM3 to discriminate the short RNA segment from other sequences, thus providing the molecular basis for the comprehension of the role of the RRM3s in the CELF/Bruno-like family.
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===Refinement of RNA binding domain 3 in CUG triplet repeat RNA-binding protein 1===
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Structural basis for the sequence-specific RNA-recognition mechanism of human CUG-BP1 RRM3.,Tsuda K, Kuwasako K, Takahashi M, Someya T, Inoue M, Terada T, Kobayashi N, Shirouzu M, Kigawa T, Tanaka A, Sugano S, Guntert P, Muto Y, Yokoyama S Nucleic Acids Res. 2009 Aug;37(15):5151-66. Epub 2009 Jun 24. PMID:19553194<ref>PMID:19553194</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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The line below this paragraph, {{ABSTRACT_PUBMED_19553194}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 2rq4" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 19553194 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_19553194}}
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__TOC__
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</StructureSection>
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==About this Structure==
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2RQ4 is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RQ4 OCA].
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==Reference==
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<ref group="xtra">PMID:19553194</ref><references group="xtra"/>
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[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Guntert, P.]]
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[[Category: Large Structures]]
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[[Category: Inoue, M.]]
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[[Category: Guntert P]]
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[[Category: Kigawa, T.]]
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[[Category: Inoue M]]
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[[Category: Kobayashi, N.]]
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[[Category: Kigawa T]]
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[[Category: Kuwasako, K.]]
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[[Category: Kobayashi N]]
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[[Category: Muto, Y.]]
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[[Category: Kuwasako K]]
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[[Category: RSGI, RIKEN Structural Genomics/Proteomics Initiative.]]
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[[Category: Muto Y]]
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[[Category: Shirouzu, M.]]
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[[Category: Shirouzu M]]
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[[Category: Someya, T.]]
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[[Category: Someya T]]
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[[Category: Takahashi, M.]]
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[[Category: Takahashi M]]
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[[Category: Terada, T.]]
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[[Category: Terada T]]
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[[Category: Tsuda, K.]]
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[[Category: Tsuda K]]
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[[Category: Yokoyama, S.]]
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[[Category: Yokoyama S]]
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[[Category: Activator]]
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[[Category: Alternative splicing]]
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[[Category: Cytoplasm]]
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[[Category: Mrna processing]]
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[[Category: National project on protein structural and functional analyse]]
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[[Category: Nppsfa]]
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[[Category: Nucleus]]
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[[Category: Phosphoprotein]]
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[[Category: Rbd]]
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[[Category: Riken structural genomics/proteomics initiative]]
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[[Category: Rna-binding]]
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[[Category: Rrm domain]]
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[[Category: Rsgi]]
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[[Category: Structural genomic]]
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[[Category: Transcription]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Aug 5 10:27:38 2009''
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Current revision

Refinement of RNA binding domain 3 in CUG triplet repeat RNA-binding protein 1

PDB ID 2rq4

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