2wpn
From Proteopedia
(Difference between revisions)
(New page: '''Unreleased structure''' The entry 2wpn is ON HOLD until sometime in the future Authors: Marques, M.M., Coelho, R., DeLacey, A.L., Pereira, I.A.C., Matias, P.M. Description: Structur...) |
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| - | '''Unreleased structure''' | ||
| - | + | ==Structure of the oxidised, as-isolated NiFeSe hydrogenase from D. vulgaris Hildenborough== | |
| + | <StructureSection load='2wpn' size='340' side='right'caption='[[2wpn]], [[Resolution|resolution]] 2.04Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[2wpn]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Desulfovibrio_vulgaris_str._Hildenborough Desulfovibrio vulgaris str. Hildenborough]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WPN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2WPN FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.04Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=FCO:CARBONMONOXIDE-(DICYANO)+IRON'>FCO</scene>, <scene name='pdbligand=FE2:FE+(II)+ION'>FE2</scene>, <scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene>, <scene name='pdbligand=OCS:CYSTEINESULFONIC+ACID'>OCS</scene>, <scene name='pdbligand=PSW:3-(SULFANYLSELANYL)-L-ALANINE'>PSW</scene>, <scene name='pdbligand=SBY:3-[DODECYL(DIMETHYL)AMMONIO]PROPANE-1-SULFONATE'>SBY</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2wpn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wpn OCA], [https://pdbe.org/2wpn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2wpn RCSB], [https://www.ebi.ac.uk/pdbsum/2wpn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2wpn ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/Q72AS4_NITV2 Q72AS4_NITV2] | ||
| + | == Evolutionary Conservation == | ||
| + | [[Image:Consurf_key_small.gif|200px|right]] | ||
| + | Check<jmol> | ||
| + | <jmolCheckbox> | ||
| + | <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/wp/2wpn_consurf.spt"</scriptWhenChecked> | ||
| + | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked> | ||
| + | <text>to colour the structure by Evolutionary Conservation</text> | ||
| + | </jmolCheckbox> | ||
| + | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2wpn ConSurf]. | ||
| + | <div style="clear:both"></div> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Hydrogen is a good energy vector, and its production from renewable sources is a requirement for its widespread use. [NiFeSe] hydrogenases (Hases) are attractive candidates for the biological production of hydrogen because they are capable of high production rates even in the presence of moderate amounts of O(2), lessening the requirements for anaerobic conditions. The three-dimensional structure of the [NiFeSe] Hase from Desulfovibrio vulgaris Hildenborough has been determined in its oxidised "as-isolated" form at 2.04-A resolution. Remarkably, this is the first structure of an oxidised Hase of the [NiFe] family that does not contain an oxide bridging ligand at the active site. Instead, an extra sulfur atom is observed binding Ni and Se, leading to a SeCys conformation that shields the NiFe site from contact with oxygen. This structure provides several insights that may explain the fast activation and O(2) tolerance of these enzymes. | ||
| - | + | The three-dimensional structure of [NiFeSe] hydrogenase from Desulfovibrio vulgaris Hildenborough: a hydrogenase without a bridging ligand in the active site in its oxidised, "as-isolated" state.,Marques MC, Coelho R, De Lacey AL, Pereira IA, Matias PM J Mol Biol. 2010 Mar 5;396(4):893-907. Epub 2009 Dec 21. PMID:20026074<ref>PMID:20026074</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| - | + | <div class="pdbe-citations 2wpn" style="background-color:#fffaf0;"></div> | |
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Desulfovibrio vulgaris str. Hildenborough]] | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Coelho R]] | ||
| + | [[Category: De Lacey AL]] | ||
| + | [[Category: Marques MC]] | ||
| + | [[Category: Matias PM]] | ||
| + | [[Category: Pereira IAC]] | ||
Current revision
Structure of the oxidised, as-isolated NiFeSe hydrogenase from D. vulgaris Hildenborough
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