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1jy6

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(New page: 200px<br /><applet load="1jy6" size="450" color="white" frame="true" align="right" spinBox="true" caption="1jy6" /> '''B4DIMERA: A DE NOVO DESIGNED FOUR-STRANDED B...)
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[[Image:1jy6.gif|left|200px]]<br /><applet load="1jy6" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1jy6" />
 
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'''B4DIMERA: A DE NOVO DESIGNED FOUR-STRANDED BETA-SHEET ASSEMBLED USING A DISULFIDE BOND'''<br />
 
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==Overview==
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==B4DIMERA: A DE NOVO DESIGNED FOUR-STRANDED BETA-SHEET ASSEMBLED USING A DISULFIDE BOND==
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The design and characterization of an open eight-stranded beta-sheet in a, synthetic, 2-fold symmetric 70-residue peptide is described. The design, strategy involves the generation of a 35-residue four-stranded beta-sheet, peptide in which successive hairpins are nucleated by appropriately, positioned (D)Pro-Xxx sequences. Oxidative dimerization using a single Cys, residue positioned at the center of the C-terminal strand results in a, disulfide-bridged eight-stranded structure. Nuclear Overhauser effects, firmly establish an eight-stranded beta-sheet in methanol. In water, the, outer strands are frayed, but a well-defined four-stranded beta-sheet, stabilized by a disulfide bridge and a hydrophobic cluster is determined, from NMR data. Comparison of the precursor peptide with the, disulfide-bridged dimer reveals considerable enhancement of beta-sheet, content in the latter, suggesting that the disulfide cross-link is an, effective strategy for the stabilization of beta-sheets.
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<StructureSection load='1jy6' size='340' side='right'caption='[[1jy6]]' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1jy6]] is a 2 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JY6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1JY6 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DPR:D-PROLINE'>DPR</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1jy6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1jy6 OCA], [https://pdbe.org/1jy6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1jy6 RCSB], [https://www.ebi.ac.uk/pdbsum/1jy6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1jy6 ProSAT]</span></td></tr>
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</table>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The design and characterization of an open eight-stranded beta-sheet in a synthetic, 2-fold symmetric 70-residue peptide is described. The design strategy involves the generation of a 35-residue four-stranded beta-sheet peptide in which successive hairpins are nucleated by appropriately positioned (D)Pro-Xxx sequences. Oxidative dimerization using a single Cys residue positioned at the center of the C-terminal strand results in a disulfide-bridged eight-stranded structure. Nuclear Overhauser effects firmly establish an eight-stranded beta-sheet in methanol. In water, the outer strands are frayed, but a well-defined four-stranded beta-sheet stabilized by a disulfide bridge and a hydrophobic cluster is determined from NMR data. Comparison of the precursor peptide with the disulfide-bridged dimer reveals considerable enhancement of beta-sheet content in the latter, suggesting that the disulfide cross-link is an effective strategy for the stabilization of beta-sheets.
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==About this Structure==
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Design and construction of an open multistranded beta-sheet polypeptide stabilized by a disulfide bridge.,Venkatraman J, Nagana Gowda GA, Balaram P J Am Chem Soc. 2002 May 8;124(18):4987-94. PMID:11982362<ref>PMID:11982362</ref>
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1JY6 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1JY6 OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Design and construction of an open multistranded beta-sheet polypeptide stabilized by a disulfide bridge., Venkatraman J, Nagana Gowda GA, Balaram P, J Am Chem Soc. 2002 May 8;124(18):4987-94. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11982362 11982362]
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</div>
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[[Category: Protein complex]]
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<div class="pdbe-citations 1jy6" style="background-color:#fffaf0;"></div>
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[[Category: Balaram, P.]]
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== References ==
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[[Category: Gowda, G.A.Nagana.]]
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<references/>
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[[Category: Venkatraman, J.]]
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__TOC__
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[[Category: de novo protein design]]
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</StructureSection>
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[[Category: disulfide bond]]
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[[Category: Large Structures]]
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[[Category: four-stranded beta-sheet]]
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[[Category: Balaram P]]
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[[Category: Nagana Gowda GA]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sun Nov 25 00:25:35 2007''
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[[Category: Venkatraman J]]

Current revision

B4DIMERA: A DE NOVO DESIGNED FOUR-STRANDED BETA-SHEET ASSEMBLED USING A DISULFIDE BOND

PDB ID 1jy6

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