1sgl

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(New page: 200px<br /><applet load="1sgl" size="450" color="white" frame="true" align="right" spinBox="true" caption="1sgl, resolution 2.20&Aring;" /> '''The three-dimensiona...)
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[[Image:1sgl.gif|left|200px]]<br /><applet load="1sgl" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1sgl, resolution 2.20&Aring;" />
 
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'''The three-dimensional structure and X-ray sequence reveal that trichomaglin is a novel S-like ribonuclease'''<br />
 
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==Overview==
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==The three-dimensional structure and X-ray sequence reveal that trichomaglin is a novel S-like ribonuclease==
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Trichomaglin is a protein isolated from root tuber of the plant Maganlin, (Trichosanthes Lepiniate, Cucurbitaceae). The crystal structure of, trichomaglin has been determined by multiple-isomorphous replacement and, refined at 2.2 A resolution. The X-ray sequence was established, based on, electron density combined with the experimentally determined N-terminal, sequence, and the sequence information derived from mass spectroscopic, analysis. X-ray sequence-based homolog search and the three-dimensional, structure reveal that trichomaglin is a novel S-like RNase, which was, confirmed by biological assay. Trichomaglin molecule contains an, additional beta sheet in the HV(b) region, compared with the known plant, RNase structures. Fourteen cystein residues form seven disulfide bridges, more than those in the other known structures of S- and S-like RNases., His43 and His105 are expected to be the catalytic acid and base, respectively. Four hydrosulfate ions are bound in the active site pocket, three of them mimicking the substrate binding sites.
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<StructureSection load='1sgl' size='340' side='right'caption='[[1sgl]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1sgl]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Trichosanthes_lepiniana Trichosanthes lepiniana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SGL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1SGL FirstGlance]. <br>
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1SGL is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Trichosanthes_lepiniana Trichosanthes lepiniana] with SOH as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1SGL OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1sgl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1sgl OCA], [https://pdbe.org/1sgl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1sgl RCSB], [https://www.ebi.ac.uk/pdbsum/1sgl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1sgl ProSAT]</span></td></tr>
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The three-dimensional structure and X-ray sequence reveal that trichomaglin is a novel S-like ribonuclease., Gan JH, Yu L, Wu J, Xu H, Choudhary JS, Blackstock WP, Liu WY, Xia ZX, Structure. 2004 Jun;12(6):1015-25. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15274921 15274921]
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</table>
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[[Category: Single protein]]
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== Function ==
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[https://www.uniprot.org/uniprot/E0CX00_TRILE E0CX00_TRILE]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/sg/1sgl_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1sgl ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Trichosanthes lepiniana]]
[[Category: Trichosanthes lepiniana]]
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[[Category: Blackstock, W.P.]]
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[[Category: Blackstock WP]]
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[[Category: Choudhary, J.S.]]
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[[Category: Choudhary JS]]
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[[Category: Gan, J.H.]]
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[[Category: Gan J-H]]
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[[Category: Liu, W.Y.]]
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[[Category: Liu W-Y]]
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[[Category: Wu, J.]]
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[[Category: Wu J]]
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[[Category: Xia, Z.X.]]
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[[Category: Xia Z-X]]
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[[Category: Xu, H.]]
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[[Category: Xu H]]
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[[Category: Yu, L.]]
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[[Category: Yu L]]
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[[Category: SOH]]
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[[Category: crystal structure]]
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[[Category: mass spectroscopic analysis]]
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[[Category: s-like ribonuclease]]
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[[Category: trichomaglin]]
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[[Category: x-ray sequence]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sun Nov 25 00:27:01 2007''
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Current revision

The three-dimensional structure and X-ray sequence reveal that trichomaglin is a novel S-like ribonuclease

PDB ID 1sgl

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