This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


2whu

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (08:45, 15 November 2023) (edit) (undo)
 
(10 intermediate revisions not shown.)
Line 1: Line 1:
-
{{Seed}}
 
-
[[Image:2whu.jpg|left|200px]]
 
-
<!--
+
==Fluorescent Protein mKeima at pH 8.0==
-
The line below this paragraph, containing "STRUCTURE_2whu", creates the "Structure Box" on the page.
+
<StructureSection load='2whu' size='340' side='right'caption='[[2whu]], [[Resolution|resolution]] 2.65&Aring;' scene=''>
-
You may change the PDB parameter (which sets the PDB file loaded into the applet)
+
== Structural highlights ==
-
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+
<table><tr><td colspan='2'>[[2whu]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Montipora_sp._20 Montipora sp. 20]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WHU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2WHU FirstGlance]. <br>
-
or leave the SCENE parameter empty for the default display.
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.65&#8491;</td></tr>
-
-->
+
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CRQ:[2-(3-CARBAMOYL-1-IMINO-PROPYL)-4-(4-HYDROXY-BENZYLIDENE)-5-OXO-4,5-DIHYDRO-IMIDAZOL-1-YL]-ACETIC+ACID'>CRQ</scene></td></tr>
-
{{STRUCTURE_2whu| PDB=2whu | SCENE= }}
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2whu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2whu OCA], [https://pdbe.org/2whu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2whu RCSB], [https://www.ebi.ac.uk/pdbsum/2whu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2whu ProSAT]</span></td></tr>
 +
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/Q1JV70_9CNID Q1JV70_9CNID]
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/wh/2whu_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2whu ConSurf].
 +
<div style="clear:both"></div>
 +
<div style="background-color:#fffaf0;">
 +
== Publication Abstract from PubMed ==
 +
The recently developed red fluorescent protein Keima exhibits the largest Stokes shift (180 nm) observed to date. Combining X-ray crystallography with (in crystallo) UV-visible absorption, fluorescence, and Raman spectroscopy, we have investigated molecular determinants of this peculiar property. The results demonstrate a pH-dependent "reverse chromophore protonation" triggered by the key residue Asp157 and which couples to cis/trans isomerization of the chromophore. These data provided guidelines to rationally design a useful Keima variant.
-
===FLUORESCENT PROTEIN MKEIMA AT PH 8.0===
+
Reverse pH-dependence of chromophore protonation explains the large Stokes shift of the red fluorescent protein mKeima.,Violot S, Carpentier P, Blanchoin L, Bourgeois D J Am Chem Soc. 2009 Aug 5;131(30):10356-7. PMID:19722611<ref>PMID:19722611</ref>
-
 
+
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-
==About this Structure==
+
</div>
-
2WHU is a 4 chains structure of sequences from [http://en.wikipedia.org/wiki/Montipora_sp._20 Montipora sp. 20]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WHU OCA].
+
<div class="pdbe-citations 2whu" style="background-color:#fffaf0;"></div>
 +
== References ==
 +
<references/>
 +
__TOC__
 +
</StructureSection>
 +
[[Category: Large Structures]]
[[Category: Montipora sp. 20]]
[[Category: Montipora sp. 20]]
-
[[Category: Blanchoin, L.]]
+
[[Category: Blanchoin L]]
-
[[Category: Bourgeois, D.]]
+
[[Category: Bourgeois D]]
-
[[Category: Carpentier, P.]]
+
[[Category: Carpentier P]]
-
[[Category: Violot, S.]]
+
[[Category: Violot S]]
-
[[Category: Fluorescent protein]]
+
-
[[Category: Mkeima]]
+
-
[[Category: Stokes shift]]
+
-
 
+
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Aug 12 13:40:42 2009''
+

Current revision

Fluorescent Protein mKeima at pH 8.0

PDB ID 2whu

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2whu"
Personal tools