3a3a
From Proteopedia
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| - | {{Seed}} | ||
| - | [[Image:3a3a.jpg|left|200px]] | ||
| - | < | + | ==Crystal structure of human selenocystine tRNA== |
| - | + | <StructureSection load='3a3a' size='340' side='right'caption='[[3a3a]], [[Resolution|resolution]] 3.10Å' scene=''> | |
| - | + | == Structural highlights == | |
| - | + | <table><tr><td colspan='2'>[[3a3a]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3A3A OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3A3A FirstGlance]. <br> | |
| - | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.1Å</td></tr> | |
| - | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3a3a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3a3a OCA], [https://pdbe.org/3a3a PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3a3a RCSB], [https://www.ebi.ac.uk/pdbsum/3a3a PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3a3a ProSAT]</span></td></tr> | |
| - | + | </table> | |
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Selenocysteine (Sec) is the 21st amino acid in translation. Sec tRNA (tRNA(Sec)) has an anticodon complementary to the UGA codon. We solved the crystal structure of human tRNA(Sec). tRNA(Sec) has a 9-bp acceptor stem and a 4-bp T stem, in contrast with the 7-bp acceptor stem and the 5-bp T stem in the canonical tRNAs. The acceptor stem is kinked between the U6:U67 and G7:C66 base pairs, leading to a bent acceptor-T stem helix. tRNA(Sec) has a 6-bp D stem and a 4-nt D loop. The long D stem includes unique A14:U21 and G15:C20a pairs. The D-loop:T-loop interactions include the base pairs G18:U55 and U16:U59, and a unique base triple, U20:G19:C56. The extra arm comprises of a 6-bp stem and a 4-nt loop. Remarkably, the D stem and the extra arm do not form tertiary interactions in tRNA(Sec). Instead, tRNA(Sec) has an open cavity, in place of the tertiary core of a canonical tRNA. The linker residues, A8 and U9, connecting the acceptor and D stems, are not involved in tertiary base pairing. Instead, U9 is stacked on the first base pair of the extra arm. These features might allow tRNA(Sec) to be the target of the Sec synthesis/incorporation machineries. | ||
| - | + | Crystal structure of human selenocysteine tRNA.,Itoh Y, Chiba S, Sekine S, Yokoyama S Nucleic Acids Res. 2009 Oct;37(18):6259-68. Epub 2009 Aug 19. PMID:19692584<ref>PMID:19692584</ref> | |
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 3a3a" style="background-color:#fffaf0;"></div> | ||
| - | == | + | ==See Also== |
| - | + | *[[Transfer RNA (tRNA)|Transfer RNA (tRNA)]] | |
| - | [[Category: | + | == References == |
| - | [[Category: | + | <references/> |
| - | [[Category: | + | __TOC__ |
| - | [[Category: | + | </StructureSection> |
| - | [[Category: | + | [[Category: Homo sapiens]] |
| - | + | [[Category: Large Structures]] | |
| - | + | [[Category: Chiba S]] | |
| + | [[Category: Itoh Y]] | ||
| + | [[Category: Sekine SI]] | ||
| + | [[Category: Yokoyama S]] | ||
Current revision
Crystal structure of human selenocystine tRNA
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