3ial

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{{Seed}}
 
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[[Image:3ial.jpg|left|200px]]
 
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==Giardia lamblia Prolyl-tRNA synthetase in complex with prolyl-adenylate==
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The line below this paragraph, containing "STRUCTURE_3ial", creates the "Structure Box" on the page.
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<StructureSection load='3ial' size='340' side='right'caption='[[3ial]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[3ial]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Giardia_lamblia_ATCC_50803 Giardia lamblia ATCC 50803]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3IAL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3IAL FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PR8:5-O-[(R)-HYDROXY{[(2S)-PYRROLIDIN-2-YLCARBONYL]OXY}PHOSPHORYL]ADENOSINE'>PR8</scene></td></tr>
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{{STRUCTURE_3ial| PDB=3ial | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3ial FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ial OCA], [https://pdbe.org/3ial PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3ial RCSB], [https://www.ebi.ac.uk/pdbsum/3ial PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3ial ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/A8BR89_GIAIC A8BR89_GIAIC]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ia/3ial_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3ial ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The genome of the human intestinal parasite Giardia lamblia contains only a single aminoacyl-tRNA synthetase gene for each amino acid. The Giardia prolyl-tRNA synthetase gene product was originally misidentified as a dual-specificity Pro/Cys enzyme, in part owing to its unexpectedly high off-target activation of cysteine, but is now believed to be a normal representative of the class of archaeal/eukaryotic prolyl-tRNA synthetases. The 2.2 A resolution crystal structure of the G. lamblia enzyme presented here is thus the first structure determination of a prolyl-tRNA synthetase from a eukaryote. The relative occupancies of substrate (proline) and product (prolyl-AMP) in the active site are consistent with half-of-the-sites reactivity, as is the observed biphasic thermal denaturation curve for the protein in the presence of proline and MgATP. However, no corresponding induced asymmetry is evident in the structure of the protein. No thermal stabilization is observed in the presence of cysteine and ATP. The implied low affinity for the off-target activation product cysteinyl-AMP suggests that translational fidelity in Giardia is aided by the rapid release of misactivated cysteine.
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===Giardia lamblia Prolyl-tRNA synthetase in complex with prolyl-adenylate===
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Structure of the prolyl-tRNA synthetase from the eukaryotic pathogen Giardia lamblia.,Larson ET, Kim JE, Napuli AJ, Verlinde CL, Fan E, Zucker FH, Van Voorhis WC, Buckner FS, Hol WG, Merritt EA Acta Crystallogr D Biol Crystallogr. 2012 Sep;68(Pt 9):1194-200. doi:, 10.1107/S0907444912024699. Epub 2012 Aug 18. PMID:22948920<ref>PMID:22948920</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 3ial" style="background-color:#fffaf0;"></div>
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==About this Structure==
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==See Also==
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3IAL is a 2 chains structure of sequences from [http://en.wikipedia.org/wiki/Giardia_lamblia_atcc_50803 Giardia lamblia atcc 50803]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3IAL OCA].
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*[[Aminoacyl tRNA synthetase 3D structures|Aminoacyl tRNA synthetase 3D structures]]
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[[Category: Giardia lamblia atcc 50803]]
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== References ==
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[[Category: Proline--tRNA ligase]]
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<references/>
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[[Category: Larson, E T.]]
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__TOC__
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[[Category: Merritt, E A.]]
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</StructureSection>
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[[Category: Protozoa, Medical Structural Genomics of Pathogenic.]]
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[[Category: Giardia lamblia ATCC 50803]]
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[[Category: Aar]]
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[[Category: Large Structures]]
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[[Category: Aminoacyl-trna synthetase]]
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[[Category: Larson ET]]
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[[Category: Atp-binding]]
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[[Category: Cysr]]
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[[Category: Ligase]]
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[[Category: Medical structural genomics of pathogenic protazoa]]
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[[Category: Medical structural genomics of pathogenic protozoa]]
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[[Category: Msgpp]]
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[[Category: Nucleotide-binding]]
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[[Category: Pror]]
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[[Category: Structural genomic]]
[[Category: Structural genomic]]
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[[Category: Translation]]
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[[Category: Merritt EA]]
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[[Category: Trna ligase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Aug 12 13:45:59 2009''
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Current revision

Giardia lamblia Prolyl-tRNA synthetase in complex with prolyl-adenylate

PDB ID 3ial

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