3ign

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{{Seed}}
 
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[[Image:3ign.jpg|left|200px]]
 
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==Crystal Structure of the GGDEF domain from Marinobacter aquaeolei diguanylate cyclase complexed with c-di-GMP - Northeast Structural Genomics Consortium Target MqR89a==
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The line below this paragraph, containing "STRUCTURE_3ign", creates the "Structure Box" on the page.
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<StructureSection load='3ign' size='340' side='right'caption='[[3ign]], [[Resolution|resolution]] 1.83&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[3ign]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Marinobacter_nauticus_VT8 Marinobacter nauticus VT8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3IGN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3IGN FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.83&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=C2E:9,9-[(2R,3R,3aS,5S,7aR,9R,10R,10aS,12S,14aR)-3,5,10,12-tetrahydroxy-5,12-dioxidooctahydro-2H,7H-difuro[3,2-d 3,2-j][1,3,7,9,2,8]tetraoxadiphosphacyclododecine-2,9-diyl]bis(2-amino-1,9-dihydro-6H-purin-6-one)'>C2E</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
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{{STRUCTURE_3ign| PDB=3ign | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3ign FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ign OCA], [https://pdbe.org/3ign PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3ign RCSB], [https://www.ebi.ac.uk/pdbsum/3ign PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3ign ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/A1U3W3_MARN8 A1U3W3_MARN8]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ig/3ign_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3ign ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Recent studies of signal transduction in bacteria have revealed a unique second messenger, bis-(3'-5')-cyclic dimeric GMP (c-di-GMP), which regulates transitions between motile states and sessile states, such as biofilms. C-di-GMP is synthesized from two GTP molecules by diguanylate cyclases (DGC). The catalytic activity of DGCs depends on a conserved GG(D/E)EF domain, usually part of a larger multi-domain protein organization. The domains other than the GG(D/E)EF domain often control DGC activation. This paper presents the 1.83 A crystal structure of an isolated catalytically competent GG(D/E)EF domain from the A1U3W3_MARAV protein from Marinobacter aquaeolei. Co-crystallization with GTP resulted in enzymatic synthesis of c-di-GMP. Comparison with previously solved DGC structures shows a similar orientation of c-di-GMP bound to an allosteric regulatory site mediating feedback inhibition of the enzyme. Biosynthesis of c-di-GMP in the crystallization reaction establishes that the enzymatic activity of this DGC domain does not require interaction with regulatory domains.
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===Crystal Structure of the GGDEF domain from Marinobacter aquaeolei diguanylate cyclase complexed with c-di-GMP - Northeast Structural Genomics Consortium Target MqR89a===
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Crystal structure of a catalytically active GG(D/E)EF diguanylate cyclase domain from Marinobacter aquaeolei with bound c-di-GMP product.,Vorobiev SM, Neely H, Yu B, Seetharaman J, Xiao R, Acton TB, Montelione GT, Hunt JF J Struct Funct Genomics. 2012 Sep;13(3):177-83. doi: 10.1007/s10969-012-9136-4., Epub 2012 Jul 29. PMID:22843345<ref>PMID:22843345</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 3ign" style="background-color:#fffaf0;"></div>
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==About this Structure==
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==See Also==
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3IGN is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Marinobacter_aquaeolei_vt8 Marinobacter aquaeolei vt8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3IGN OCA].
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*[[Diguanylate cyclase|Diguanylate cyclase]]
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[[Category: Diguanylate kinase]]
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== References ==
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[[Category: Marinobacter aquaeolei vt8]]
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<references/>
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[[Category: Acton, T B.]]
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__TOC__
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[[Category: Ciccosanti, C.]]
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</StructureSection>
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[[Category: Foote, E L.]]
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[[Category: Large Structures]]
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[[Category: Hunt, J F.]]
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[[Category: Marinobacter nauticus VT8]]
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[[Category: Montelione, G T.]]
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[[Category: Acton TB]]
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[[Category: NESG, Northeast Structural Genomics Consortium.]]
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[[Category: Ciccosanti C]]
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[[Category: Neely, H.]]
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[[Category: Foote EL]]
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[[Category: Sahdev, S.]]
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[[Category: Hunt JF]]
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[[Category: Seetharaman, J.]]
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[[Category: Montelione GT]]
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[[Category: Tong, L.]]
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[[Category: Neely H]]
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[[Category: Vorobiev, S.]]
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[[Category: Sahdev S]]
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[[Category: Wang, H.]]
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[[Category: Seetharaman J]]
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[[Category: Xiao, R.]]
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[[Category: Tong L]]
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[[Category: A1u3w3_marav]]
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[[Category: Vorobiev S]]
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[[Category: Diguanylate cyclase]]
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[[Category: Wang H]]
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[[Category: Ggdef domain]]
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[[Category: Xiao R]]
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[[Category: Mqr89a]]
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[[Category: Nesg]]
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[[Category: Northeast structural genomics consortium]]
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[[Category: Protein structure initiative]]
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[[Category: Psi-2]]
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[[Category: Structural genomic]]
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[[Category: Transferase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Aug 12 13:47:47 2009''
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Current revision

Crystal Structure of the GGDEF domain from Marinobacter aquaeolei diguanylate cyclase complexed with c-di-GMP - Northeast Structural Genomics Consortium Target MqR89a

PDB ID 3ign

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