3ec2

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{{Seed}}
 
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[[Image:3ec2.png|left|200px]]
 
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==Crystal structure of the DnaC helicase loader==
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The line below this paragraph, containing "STRUCTURE_3ec2", creates the "Structure Box" on the page.
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<StructureSection load='3ec2' size='340' side='right'caption='[[3ec2]], [[Resolution|resolution]] 2.70&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[3ec2]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Aquifex_aeolicus Aquifex aeolicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3EC2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3EC2 FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.7&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
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{{STRUCTURE_3ec2| PDB=3ec2 | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3ec2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ec2 OCA], [https://pdbe.org/3ec2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3ec2 RCSB], [https://www.ebi.ac.uk/pdbsum/3ec2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3ec2 ProSAT]</span></td></tr>
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</table>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ec/3ec2_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3ec2 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The loading of oligomeric helicases onto replication origins marks an essential step in replisome assembly. In cells, dedicated AAA+ ATPases regulate loading, however, the mechanism by which these factors recruit and deposit helicases has remained unclear. To better understand this process, we determined the structure of the ATPase region of the bacterial helicase loader DnaC from Aquifex aeolicus to 2.7 A resolution. The structure shows that DnaC is a close paralog of the bacterial replication initiator, DnaA, and unexpectedly shares an ability to form a helical assembly similar to that of ATP-bound DnaA. Complementation and ssDNA-binding assays validate the importance of homomeric DnaC interactions, while pull-down experiments show that the DnaC and DnaA AAA+ domains interact in a nucleotide-dependent manner. These findings implicate DnaC as a molecular adaptor that uses ATP-activated DnaA as a docking site for regulating the recruitment and correct spatial deposition of the DnaB helicase onto origins.
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===Crystal structure of the DnaC helicase loader===
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Structural synergy and molecular crosstalk between bacterial helicase loaders and replication initiators.,Mott ML, Erzberger JP, Coons MM, Berger JM Cell. 2008 Nov 14;135(4):623-34. PMID:19013274<ref>PMID:19013274</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 3ec2" style="background-color:#fffaf0;"></div>
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==About this Structure==
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==See Also==
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3EC2 is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Aquifex_aeolicus Aquifex aeolicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3EC2 OCA].
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*[[DnaC helicase loader|DnaC helicase loader]]
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*[[Helicase 3D structures|Helicase 3D structures]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
[[Category: Aquifex aeolicus]]
[[Category: Aquifex aeolicus]]
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[[Category: Berger, J M.]]
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[[Category: Large Structures]]
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[[Category: Coons, M M.]]
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[[Category: Berger JM]]
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[[Category: Erzberger, J P.]]
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[[Category: Coons MM]]
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[[Category: Mott, M L.]]
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[[Category: Erzberger JP]]
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[[Category: Atp-binding]]
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[[Category: Mott ML]]
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[[Category: Helicase loader]]
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[[Category: Nucleotide-binding]]
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[[Category: Replication initiation factor]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Aug 12 20:04:56 2009''
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Current revision

Crystal structure of the DnaC helicase loader

PDB ID 3ec2

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