1g00

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(New page: 200px<br /><applet load="1g00" size="450" color="white" frame="true" align="right" spinBox="true" caption="1g00, resolution 2.3&Aring;" /> '''ALTERNATION OF DNA AN...)
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[[Image:1g00.gif|left|200px]]<br /><applet load="1g00" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1g00, resolution 2.3&Aring;" />
 
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'''ALTERNATION OF DNA AND SOLVENT LAYERS IN THE A FORM OF D(GGCGCC) OBTAINED BY ETHANOL CRYSTALLIZATION'''<br />
 
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==Overview==
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==ALTERNATION OF DNA AND SOLVENT LAYERS IN THE A FORM OF D(GGCGCC) OBTAINED BY ETHANOL CRYSTALLIZATION==
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We have determined by X-ray crystallography the structure of the hexamer, duplex d(GGCGCC)2 in the A-form using ethanol as a precipitant. The same, sequence had previously been crystallized in the B-form, but with, 2-methyl-2,4-pentanediol as a precipitant. It appears that ethanol, precipitation is a useful method to induce the formation of A-form, crystals of DNA. Packing of the molecules in the crystal has unique, features: the known interaction of A-DNA duplexes between terminal, base-pairs and the minor groove of neighbor molecules is combined with a, superstructure consisting in an alternation of DNA layers and solvent, layers (water/ions). This organization in layers has been observed before, also with hexamers in the A conformation which crystallize in the same, space group (C2221). The solvent layer has a precise thickness, although, very few ordered water molecules can be detected. Another feature of this, crystal is its large unit cell, which gives rise to an asymmetric unit, with three hexamer duplexes. One of the three duplexes is quite different, from the other two in several aspects: the number of base pairs per turn, the twist pattern, the mean value of the twist angle and the fact that one, terminal base-pair is not stacked as part of the duplex and appears to be, disordered. So the variability in conformation of this sequence is, remarkable.
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<StructureSection load='1g00' size='340' side='right'caption='[[1g00]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1g00]] is a 6 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1G00 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1G00 FirstGlance]. <br>
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1G00 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1G00 OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1g00 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1g00 OCA], [https://pdbe.org/1g00 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1g00 RCSB], [https://www.ebi.ac.uk/pdbsum/1g00 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1g00 ProSAT]</span></td></tr>
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==Reference==
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</table>
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Alternation of DNA and solvent layers in the A form of d(GGCGCC) obtained by ethanol crystallization., Urpi L, Navaza J, Subirana JA, J Biomol Struct Dyn. 2000 Dec;18(3):363-9. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11149513 11149513]
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__TOC__
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[[Category: Protein complex]]
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</StructureSection>
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[[Category: Navaza, J.]]
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[[Category: Large Structures]]
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[[Category: Subirana, J.A.]]
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[[Category: Navaza J]]
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[[Category: Urpi, L.]]
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[[Category: Subirana JA]]
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[[Category: a-dna]]
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[[Category: Urpi L]]
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[[Category: dna layers]]
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[[Category: double helix]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sun Nov 25 00:36:09 2007''
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ALTERNATION OF DNA AND SOLVENT LAYERS IN THE A FORM OF D(GGCGCC) OBTAINED BY ETHANOL CRYSTALLIZATION

PDB ID 1g00

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