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1o9z

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(New page: 200px<br /><applet load="1o9z" size="450" color="white" frame="true" align="right" spinBox="true" caption="1o9z, resolution 1.75&Aring;" /> '''F17-AG LECTIN DOMAIN...)
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[[Image:1o9z.jpg|left|200px]]<br /><applet load="1o9z" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1o9z, resolution 1.75&Aring;" />
 
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'''F17-AG LECTIN DOMAIN FROM ESCHERICHIA COLI (LIGAND FREE)'''<br />
 
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==Overview==
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==F17-aG lectin domain from Escherichia coli (ligand free)==
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The F17-G adhesin at the tip of flexible F17 fimbriae of enterotoxigenic, Escherichia coli mediates binding to, N-acetyl-beta-D-glucosamine-presenting receptors on the microvilli of the, intestinal epithelium of ruminants. We report the 1.7 A resolution crystal, structure of the lectin domain of F17-G, both free and in complex with, N-acetylglucosamine. The monosaccharide is bound on the side of the, ellipsoid-shaped protein in a conserved site around which all natural, variations of F17-G are clustered. A model is proposed for the interaction, between F17-fimbriated E. coli and microvilli with enhanced affinity, compared with the binding constant we determined for F17-G binding to, N-acetylglucosamine (0.85 mM-1). Unexpectedly, the F17-G structure reveals, that the lectin domains of the F17-G, PapGII and FimH fimbrial adhesins, all share the immunoglobulin-like fold of the structural components, (pilins) of their fimbriae, despite lack of any sequence identity. Fold, comparisons with pilin and chaperone structures of the chaperone/usher, pathway highlight the central role of the C-terminal beta-strand G of the, immunoglobulin-like fold and provides new insights into pilus assembly, function and adhesion.
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<StructureSection load='1o9z' size='340' side='right'caption='[[1o9z]], [[Resolution|resolution]] 1.75&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1o9z]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1O9Z OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1O9Z FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.75&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1o9z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1o9z OCA], [https://pdbe.org/1o9z PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1o9z RCSB], [https://www.ebi.ac.uk/pdbsum/1o9z PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1o9z ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/F17AG_ECOLX F17AG_ECOLX] Essential fimbrial adhesion factor that mediates binding to N-acetylglucosamine-containing receptors in the host intestinal microvilli, leading to colonization of the intestinal tissue, and diarrhea or septicemia. Also confers adhesiveness to laminin and basement membranes.
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The F17-G adhesin at the tip of flexible F17 fimbriae of enterotoxigenic Escherichia coli mediates binding to N-acetyl-beta-D-glucosamine-presenting receptors on the microvilli of the intestinal epithelium of ruminants. We report the 1.7 A resolution crystal structure of the lectin domain of F17-G, both free and in complex with N-acetylglucosamine. The monosaccharide is bound on the side of the ellipsoid-shaped protein in a conserved site around which all natural variations of F17-G are clustered. A model is proposed for the interaction between F17-fimbriated E. coli and microvilli with enhanced affinity compared with the binding constant we determined for F17-G binding to N-acetylglucosamine (0.85 mM-1). Unexpectedly, the F17-G structure reveals that the lectin domains of the F17-G, PapGII and FimH fimbrial adhesins all share the immunoglobulin-like fold of the structural components (pilins) of their fimbriae, despite lack of any sequence identity. Fold comparisons with pilin and chaperone structures of the chaperone/usher pathway highlight the central role of the C-terminal beta-strand G of the immunoglobulin-like fold and provides new insights into pilus assembly, function and adhesion.
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==About this Structure==
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The fimbrial adhesin F17-G of enterotoxigenic Escherichia coli has an immunoglobulin-like lectin domain that binds N-acetylglucosamine.,Buts L, Bouckaert J, De Genst E, Loris R, Oscarson S, Lahmann M, Messens J, Brosens E, Wyns L, De Greve H Mol Microbiol. 2003 Aug;49(3):705-15. PMID:12864853<ref>PMID:12864853</ref>
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1O9Z is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1O9Z OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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The fimbrial adhesin F17-G of enterotoxigenic Escherichia coli has an immunoglobulin-like lectin domain that binds N-acetylglucosamine., Buts L, Bouckaert J, De Genst E, Loris R, Oscarson S, Lahmann M, Messens J, Brosens E, Wyns L, De Greve H, Mol Microbiol. 2003 Aug;49(3):705-15. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12864853 12864853]
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</div>
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[[Category: Escherichia coli]]
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<div class="pdbe-citations 1o9z" style="background-color:#fffaf0;"></div>
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[[Category: Single protein]]
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[[Category: Bouckaert, J.]]
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[[Category: Brosens, E.]]
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[[Category: Buts, L.]]
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[[Category: Genst, E.De.]]
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[[Category: Greve, H.De.]]
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[[Category: Lahmann, M.]]
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[[Category: Loris, R.]]
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[[Category: Messens, J.]]
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[[Category: Oscarson, S.]]
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[[Category: Wyns, L.]]
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[[Category: bacterial adhesin]]
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[[Category: bacterial attachment]]
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[[Category: immunoglobulin fold]]
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[[Category: lectin]]
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[[Category: pathogenesis]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sun Nov 25 00:37:06 2007''
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==See Also==
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*[[Adhesin 3D structures|Adhesin 3D structures]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Escherichia coli]]
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[[Category: Large Structures]]
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[[Category: Bouckaert J]]
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[[Category: Brosens E]]
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[[Category: Buts L]]
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[[Category: De Genst E]]
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[[Category: De Greve H]]
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[[Category: Lahmann M]]
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[[Category: Loris R]]
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[[Category: Messens J]]
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[[Category: Oscarson S]]
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[[Category: Wyns L]]

Current revision

F17-aG lectin domain from Escherichia coli (ligand free)

PDB ID 1o9z

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