3dhd

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{{Seed}}
 
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[[Image:3dhd.jpg|left|200px]]
 
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==Crystal structure of human NAMPT complexed with nicotinamide mononucleotide and pyrophosphate==
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The line below this paragraph, containing "STRUCTURE_3dhd", creates the "Structure Box" on the page.
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<StructureSection load='3dhd' size='340' side='right'caption='[[3dhd]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[3dhd]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3DHD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3DHD FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NMN:BETA-NICOTINAMIDE+RIBOSE+MONOPHOSPHATE'>NMN</scene>, <scene name='pdbligand=POP:PYROPHOSPHATE+2-'>POP</scene></td></tr>
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{{STRUCTURE_3dhd| PDB=3dhd | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3dhd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3dhd OCA], [https://pdbe.org/3dhd PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3dhd RCSB], [https://www.ebi.ac.uk/pdbsum/3dhd PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3dhd ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/NAMPT_HUMAN NAMPT_HUMAN] Catalyzes the condensation of nicotinamide with 5-phosphoribosyl-1-pyrophosphate to yield nicotinamide mononucleotide, an intermediate in the biosynthesis of NAD. It is the rate limiting component in the mammalian NAD biosynthesis pathway (By similarity).
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/dh/3dhd_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3dhd ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Nicotinamide phosphoribosyltransferase (NAMPT) is highly evolved to capture nicotinamide (NAM) and replenish the nicotinamide adenine dinucleotide (NAD(+)) pool during ADP-ribosylation and transferase reactions. ATP-phosphorylation of an active-site histidine causes catalytic activation, increasing NAM affinity by 160,000. Crystal structures of NAMPT with catalytic site ligands identify the phosphorylation site, establish its role in catalysis, demonstrate unique overlapping ATP and phosphoribosyltransferase sites, and establish reaction coordinate motion. NAMPT structures with beryllium fluoride indicate a covalent H247-BeF(3)(-) as the phosphohistidine mimic. Activation of NAMPT by H247-phosphorylation causes stabilization of the enzyme-phosphoribosylpyrophosphate complex, permitting efficient capture of NAM. Reactant and product structures establish reaction coordinate motion for NAMPT to be migration of the ribosyl anomeric carbon from the pyrophosphate leaving group to the nicotinamide-N1 while the 5-phosphoryl group, the pyrophosphate moiety, and the nicotinamide ring remain fixed in the catalytic site.
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===Crystal structure of human NAMPT complexed with nicotinamide mononucleotide and pyrophosphate===
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A phosphoenzyme mimic, overlapping catalytic sites and reaction coordinate motion for human NAMPT.,Burgos ES, Ho MC, Almo SC, Schramm VL Proc Natl Acad Sci U S A. 2009 Aug 4. PMID:19666527<ref>PMID:19666527</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 3dhd" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_19666527}}, adds the Publication Abstract to the page
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*[[Phosphoribosyltransferase 3D structures|Phosphoribosyltransferase 3D structures]]
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(as it appears on PubMed at http://www.pubmed.gov), where 19666527 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_19666527}}
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__TOC__
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</StructureSection>
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==About this Structure==
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3DHD is a 2 chains structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3DHD OCA].
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==Reference==
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<ref group="xtra">PMID:19666527</ref><references group="xtra"/>
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[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Nicotinamide phosphoribosyltransferase]]
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[[Category: Large Structures]]
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[[Category: Almo, S C.]]
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[[Category: Almo SC]]
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[[Category: Burgos, E S.]]
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[[Category: Burgos ES]]
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[[Category: Ho, M.]]
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[[Category: Ho M]]
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[[Category: Schramm, V L.]]
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[[Category: Schramm VL]]
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[[Category: Alternative splicing]]
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[[Category: Cytoplasm]]
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[[Category: Glycosyltransferase]]
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[[Category: Namprtase]]
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[[Category: Nicotinamide d-ribonucleotide]]
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[[Category: Nmn]]
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[[Category: Nmprtase]]
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[[Category: Phosphoprotein]]
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[[Category: Polymorphism]]
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[[Category: Pyridine nucleotide biosynthesis]]
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[[Category: Pyrophosphate]]
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[[Category: Transferase]]
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[[Category: Visfatin]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Aug 19 13:11:21 2009''
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Current revision

Crystal structure of human NAMPT complexed with nicotinamide mononucleotide and pyrophosphate

PDB ID 3dhd

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