1k2l

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(New page: 200px<br /><applet load="1k2l" size="450" color="white" frame="true" align="right" spinBox="true" caption="1k2l, resolution 2.40&Aring;" /> '''STRUCTURAL CHARACTER...)
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[[Image:1k2l.gif|left|200px]]<br /><applet load="1k2l" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1k2l, resolution 2.40&Aring;" />
 
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'''STRUCTURAL CHARACTERIZATION OF BISINTERCALATION IN HIGHER-ORDER DNA AT A JUNCTION-LIKE QUADRUPLEX'''<br />
 
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==Overview==
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==STRUCTURAL CHARACTERIZATION OF BISINTERCALATION IN HIGHER-ORDER DNA AT A JUNCTION-LIKE QUADRUPLEX==
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We report the single-crystal X-ray structure for the complex of the, bisacridine, bis-(9-aminooctyl(2-(dimethylaminoethyl)acridine-4-carboxamide)) with the, oligonucleotide d(CGTACG)(2) to a resolution of 2.4A. Solution studies, with closed circular DNA show this compound to be a bisintercalating, threading agent, but so far we have no crystallographic or NMR structural, data conforming to the model of contiguous intercalation within the same, duplex. Here, with the hexameric duplex d(CGTACG), the DNA is observed to, undergo a terminal cytosine base exchange to yield an unusual guanine, quadruplex intercalation site through which the bisacridine threads its, octamethylene linker to fuse two DNA duplexes. The 4-carboxamide, side-chains form anchoring hydrogen-bonding interactions with guanine O6, atoms on each side of the quadruplex. This higher-order DNA structure, provides insight into an unexpected property of bisintercalating threading, agents, and suggests the idea of targeting such compounds specifically at, four-way DNA junctions.
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<StructureSection load='1k2l' size='340' side='right'caption='[[1k2l]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1k2l]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1K2L OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1K2L FirstGlance]. <br>
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1K2L is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with BDC and CO as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1K2L OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BDC:BIS-(9-OCTYLAMINO(2-DIMETHYLAMINOETHYL)ACRIDINE-4-CARBOXAMIDE'>BDC</scene>, <scene name='pdbligand=CO:COBALT+(II)+ION'>CO</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1k2l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1k2l OCA], [https://pdbe.org/1k2l PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1k2l RCSB], [https://www.ebi.ac.uk/pdbsum/1k2l PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1k2l ProSAT]</span></td></tr>
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Structural characterisation of bisintercalation in higher-order DNA at a junction-like quadruplex., Teixeira SC, Thorpe JH, Todd AK, Powell HR, Adams A, Wakelin LP, Denny WA, Cardin CJ, J Mol Biol. 2002 Oct 18;323(2):167-71. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12381312 12381312]
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</table>
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[[Category: Protein complex]]
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__TOC__
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[[Category: Adams, A.]]
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</StructureSection>
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[[Category: Cardin, C.J.]]
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[[Category: Large Structures]]
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[[Category: Denny, W.A.]]
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[[Category: Adams A]]
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[[Category: Powell, H.R.]]
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[[Category: Cardin CJ]]
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[[Category: Teixeira, S.C.M.]]
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[[Category: Denny WA]]
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[[Category: Thorpe, J.H.]]
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[[Category: Powell HR]]
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[[Category: Todd, A.K.]]
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[[Category: Teixeira SCM]]
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[[Category: Wakelin, L.P.G.]]
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[[Category: Thorpe JH]]
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[[Category: BDC]]
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[[Category: Todd AK]]
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[[Category: CO]]
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[[Category: Wakelin LPG]]
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[[Category: bisintercalation]]
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[[Category: dna strand exchange]]
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[[Category: quadruplex]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sun Nov 25 00:38:03 2007''
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STRUCTURAL CHARACTERIZATION OF BISINTERCALATION IN HIGHER-ORDER DNA AT A JUNCTION-LIKE QUADRUPLEX

PDB ID 1k2l

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