1bvo

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(New page: 200px<br /><applet load="1bvo" size="450" color="white" frame="true" align="right" spinBox="true" caption="1bvo, resolution 2.70&Aring;" /> '''DORSAL HOMOLOGUE GAM...)
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[[Image:1bvo.gif|left|200px]]<br /><applet load="1bvo" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1bvo, resolution 2.70&Aring;" />
 
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'''DORSAL HOMOLOGUE GAMBIF1 BOUND TO DNA'''<br />
 
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==Overview==
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==DORSAL HOMOLOGUE GAMBIF1 BOUND TO DNA==
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BACKGROUND: NF-kappa B/Rel transcription factors play important roles in, immunity and development in mammals and insects. Their activity is, regulated by their cellular localization, homo- and heterodimerization and, association with other factors on their target gene promoters. Gambif1, from Anopheles gambiae is a member of the Rel family and a close homologue, of the morphogen Dorsal, which establishes dorsoventral polarity in the, Drosophila embryo. RESULTS: We present the crystal structure of the, N-terminal specificity domain of Gambif1 bound to DNA. This first, structure of an insect Rel protein-DNA complex shows that Gambif1 binds a, GGG half-site element using a stack of three arginine sidechains., Differences in affinity to Dorsal binding sites in target gene promoters, are predicted to arise from base changes in these GGG elements. An, arginine that is conserved in class II Rel proteins (members of which, contain a transcription activation domain) contacts the outermost guanines, of the DNA site. This previously unseen specific contact contributes, strongly to the DNA-binding affinity and might be responsible for, differences in specificity between Rel proteins of class I and II., CONCLUSIONS: The Gambif1-DNA complex structure illustrates how differences, in Dorsal affinity to binding sites in developmental gene promoters are, achieved. Comparison with other Rel-DNA complex structures leads to a, general model for DNA recognition by Rel proteins.
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<StructureSection load='1bvo' size='340' side='right'caption='[[1bvo]], [[Resolution|resolution]] 2.70&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1bvo]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Anopheles_gambiae Anopheles gambiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BVO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1BVO FirstGlance]. <br>
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1BVO is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Anopheles_gambiae Anopheles gambiae]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1BVO OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.7&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1bvo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1bvo OCA], [https://pdbe.org/1bvo PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1bvo RCSB], [https://www.ebi.ac.uk/pdbsum/1bvo PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1bvo ProSAT]</span></td></tr>
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==Reference==
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</table>
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Structure of the specificity domain of the Dorsal homologue Gambif1 bound to DNA., Cramer P, Varrot A, Barillas-Mury C, Kafatos FC, Muller CW, Structure. 1999 Jul 15;7(7):841-52. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10425685 10425685]
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== Function ==
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[https://www.uniprot.org/uniprot/Q17034_ANOGA Q17034_ANOGA]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/bv/1bvo_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1bvo ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
[[Category: Anopheles gambiae]]
[[Category: Anopheles gambiae]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Barillas-Mury, C.]]
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[[Category: Barillas-Mury C]]
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[[Category: Cramer, P.]]
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[[Category: Cramer P]]
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[[Category: Kafatos, F.C.]]
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[[Category: Kafatos FC]]
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[[Category: Mueller, C.W.]]
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[[Category: Mueller CW]]
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[[Category: Varrot, A.]]
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[[Category: Varrot A]]
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[[Category: complex (transcription factor/dna)]]
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[[Category: development]]
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[[Category: immunity]]
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[[Category: insects]]
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[[Category: morphogen]]
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[[Category: rel protein]]
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[[Category: transcription factor]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sun Nov 25 00:39:59 2007''
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Current revision

DORSAL HOMOLOGUE GAMBIF1 BOUND TO DNA

PDB ID 1bvo

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