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1bwt

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(New page: 200px<br /><applet load="1bwt" size="450" color="white" frame="true" align="right" spinBox="true" caption="1bwt" /> '''NMR SOLUTION STRUCTURE OF [D(GCGAATCGC)2]'''...)
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'''NMR SOLUTION STRUCTURE OF [D(GCGAATCGC)2]'''<br />
 
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==Overview==
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==NMR SOLUTION STRUCTURE OF [D(GCGAATCGC)2]==
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We present the high-resolution solution structures of a self-complementary, DNA decamer duplex featuring a single alpha-anomeric nucleotide per strand, encompassed by a set of 3'-3' and 5'-5' phosphodiester linkages, d(GCGAAT-3'-3'-alphaT-5'-5'-CGC)2, alphaT, and its unmodified control, d(GCGAATTCGC)2, obtained by restrained molecular dynamics. Interproton, distance and deoxyribose ring torsion angle restraints were deduced from, homonuclear NOESY and DQF-COSY data, respectively. For both the control, and alphaT duplexes, excellent global convergence was observed from two, different (A- and B-) starting models. The final average structures of the, two duplexes are highly homologous, and overall possess the traits, characteristic of right-handed B-DNA duplexes. However, localized, differences between the two structures stem from the enhanced, conformational exchange in the deoxyribose ring of the cytidine following, the 5'-5' linkage, the C3'- exo pseudorotation phase angle of the, alpha-nucleotide, and unusual backbone torsions in the 3'-3' and 5'-5', phosphodiester linkages. The structural data reported here are relevant to, the design of antisense therapeutics comprised of these modifications.
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<StructureSection load='1bwt' size='340' side='right'caption='[[1bwt]]' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1bwt]] is a 2 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BWT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1BWT FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1bwt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1bwt OCA], [https://pdbe.org/1bwt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1bwt RCSB], [https://www.ebi.ac.uk/pdbsum/1bwt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1bwt ProSAT]</span></td></tr>
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</table>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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We present the high-resolution solution structures of a self-complementary DNA decamer duplex featuring a single alpha-anomeric nucleotide per strand encompassed by a set of 3'-3' and 5'-5' phosphodiester linkages, d(GCGAAT-3'-3'-alphaT-5'-5'-CGC)2, alphaT, and its unmodified control, d(GCGAATTCGC)2, obtained by restrained molecular dynamics. Interproton distance and deoxyribose ring torsion angle restraints were deduced from homonuclear NOESY and DQF-COSY data, respectively. For both the control and alphaT duplexes, excellent global convergence was observed from two different (A- and B-) starting models. The final average structures of the two duplexes are highly homologous, and overall possess the traits characteristic of right-handed B-DNA duplexes. However, localized differences between the two structures stem from the enhanced conformational exchange in the deoxyribose ring of the cytidine following the 5'-5' linkage, the C3'- exo pseudorotation phase angle of the alpha-nucleotide, and unusual backbone torsions in the 3'-3' and 5'-5' phosphodiester linkages. The structural data reported here are relevant to the design of antisense therapeutics comprised of these modifications.
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==About this Structure==
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NMR solution structures of [d(GCGAAT-3'-3'-alphaT-5'-5'-CGC)2] and its unmodified control.,Aramini JM, Mujeeb A, Germann MW Nucleic Acids Res. 1998 Dec 15;26(24):5644-54. PMID:9837995<ref>PMID:9837995</ref>
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1BWT is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1BWT OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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NMR solution structures of [d(GCGAAT-3'-3'-alphaT-5'-5'-CGC)2] and its unmodified control., Aramini JM, Mujeeb A, Germann MW, Nucleic Acids Res. 1998 Dec 15;26(24):5644-54. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9837995 9837995]
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</div>
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[[Category: Protein complex]]
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<div class="pdbe-citations 1bwt" style="background-color:#fffaf0;"></div>
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[[Category: Aramini, J.M.]]
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== References ==
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[[Category: Germann, M.W.]]
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<references/>
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[[Category: Mujeeb, A.]]
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__TOC__
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[[Category: ecori recognition site]]
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</StructureSection>
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[[Category: Large Structures]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sun Nov 25 00:43:11 2007''
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[[Category: Aramini JM]]
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[[Category: Germann MW]]
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[[Category: Mujeeb A]]

Current revision

NMR SOLUTION STRUCTURE OF [D(GCGAATCGC)2]

PDB ID 1bwt

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