2wn4

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{{Seed}}
 
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[[Image:2wn4.png|left|200px]]
 
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==Structural Basis for Substrate Recognition in the Enzymatic Component of ADP-ribosyltransferase Toxin CDTa from Clostridium difficile==
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The line below this paragraph, containing "STRUCTURE_2wn4", creates the "Structure Box" on the page.
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<StructureSection load='2wn4' size='340' side='right'caption='[[2wn4]], [[Resolution|resolution]] 1.85&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2wn4]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Clostridioides_difficile Clostridioides difficile]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WN4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2WN4 FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.85&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2wn4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wn4 OCA], [https://pdbe.org/2wn4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2wn4 RCSB], [https://www.ebi.ac.uk/pdbsum/2wn4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2wn4 ProSAT]</span></td></tr>
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{{STRUCTURE_2wn4| PDB=2wn4 | SCENE= }}
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/Q9KH42_CLODI Q9KH42_CLODI]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/wn/2wn4_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2wn4 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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ADP-ribosylation is one of the favored modes of cell intoxication employed by several bacteria. Clostridium difficile is recognized to be an important nosocomial pathogen associated with considerable morbidity and attributable mortality. Along with its two well known toxins, Toxin A and Toxin B, it produces an ADP-ribosylating toxin that targets monomeric actin of the target cell. Like other Clostridial actin ADP-ribosylating toxins, this binary toxin, known as C. difficile toxin (CDT), is composed of two subunits, CDTa and CDTb. In this study, we present high resolution crystal structures of CDTa in its native form (at pH 4.0, 8.5, and 9.0) and in complex with ADP-ribose donors, NAD and NADPH (at pH 9.0). The crystal structures of the native protein show "pronounced conformational flexibility" confined to the active site region of the protein and "enhanced" disorder at low pH, whereas the complex structures highlight significant differences in "ligand specificity" compared with the enzymatic subunit of a close homologue, Clostridium perfringens iota toxin. Specifically in CDTa, two of the suggested catalytically important residues (Glu-385 and Glu-387) seem to play no role or a less important role in ligand binding. These structural data provide the first detailed information on protein-donor substrate complex stabilization in CDTa, which may have implications in understanding CDT recognition.
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===STRUCTURAL BASIS FOR SUBSTRATE RECOGNITION IN THE ENZYMATIC COMPONENT OF ADP-RIBOSYLTRANSFERASE TOXIN CDTA FROM CLOSTRIDIUM DIFFICILE===
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Structural basis for substrate recognition in the enzymatic component of ADP-ribosyltransferase toxin CDTa from Clostridium difficile.,Sundriyal A, Roberts AK, Shone CC, Acharya KR J Biol Chem. 2009 Oct 16;284(42):28713-9. Epub 2009 Aug 19. PMID:19692332<ref>PMID:19692332</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_19692332}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 2wn4" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 19692332 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_19692332}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Clostridioides difficile]]
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2WN4 is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Clostridium_difficile Clostridium difficile]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WN4 OCA].
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[[Category: Large Structures]]
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[[Category: Acharya KR]]
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==Reference==
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[[Category: Roberts AK]]
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<ref group="xtra">PMID:19692332</ref><references group="xtra"/>
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[[Category: Shone CC]]
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[[Category: Clostridium difficile]]
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[[Category: Sundriyal A]]
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[[Category: Acharya, K R.]]
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[[Category: Roberts, A K.]]
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[[Category: Shone, C C.]]
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[[Category: Sundriyal, A.]]
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[[Category: Actin-adprt]]
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[[Category: Binary toxin]]
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[[Category: Cdta]]
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[[Category: Ribosyltransferase]]
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[[Category: Transferase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Sep 3 15:32:49 2009''
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Current revision

Structural Basis for Substrate Recognition in the Enzymatic Component of ADP-ribosyltransferase Toxin CDTa from Clostridium difficile

PDB ID 2wn4

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