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2wiy

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{{Seed}}
 
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[[Image:2wiy.png|left|200px]]
 
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==Cytochrome P450 XplA heme domain P21212==
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The line below this paragraph, containing "STRUCTURE_2wiy", creates the "Structure Box" on the page.
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<StructureSection load='2wiy' size='340' side='right'caption='[[2wiy]], [[Resolution|resolution]] 1.49&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2wiy]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Rhodococcus Rhodococcus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WIY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2WIY FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.49&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=IMD:IMIDAZOLE'>IMD</scene></td></tr>
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{{STRUCTURE_2wiy| PDB=2wiy | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2wiy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wiy OCA], [https://pdbe.org/2wiy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2wiy RCSB], [https://www.ebi.ac.uk/pdbsum/2wiy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2wiy ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/Q8GPH7_RHORH Q8GPH7_RHORH]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/wi/2wiy_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2wiy ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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XplA is a cytochrome P450 of unique structural organization, consisting of a heme-domain that is C-terminally fused to its native flavodoxin redox partner. XplA, along with flavodoxin reductase XplB, has been shown to catalyze the breakdown of the nitramine explosive and pollutant hexahydro-1,3,5-trinitro-1,3,5-triazine (royal demolition explosive) by reductive denitration. The structure of the heme domain of XplA (XplA-heme) has been solved in two crystal forms: as a dimer in space group P2(1) to a resolution of 1.9 A and as a monomer in space group P2(1)2(1)2 to a resolution of 1.5 A, with the ligand imidazole bound at the heme iron. Although it shares the overall fold of cytochromes P450 of known structure, XplA-heme is unusual in that the kinked I-helix that traverses the distal face of the heme is broken by Met-394 and Ala-395 in place of the well conserved Asp/Glu plus Thr/Ser, important in oxidative P450s for the scission of the dioxygen bond prior to substrate oxygenation. The heme environment of XplA-heme is hydrophobic, featuring a cluster of three methionines above the heme, including Met-394. Imidazole was observed bound to the heme iron and is in close proximity to the side chain of Gln-438, which is situated over the distal face of the heme. Imidazole is also hydrogen-bonded to a water molecule that sits in place of the threonine side-chain hydroxyl exemplified by Thr-252 in Cyt-P450cam. Both Gln-438 --&gt; Ala and Ala-395 --&gt; Thr mutants of XplA-heme displayed markedly reduced activity compared with the wild type for royal demolition explosive degradation when combined with surrogate electron donors.
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===CYTOCHROME P450 XPLA HEME DOMAIN P21212===
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The 1.5-A structure of XplA-heme, an unusual cytochrome P450 heme domain that catalyzes reductive biotransformation of royal demolition explosive.,Sabbadin F, Jackson R, Haider K, Tampi G, Turkenburg JP, Hart S, Bruce NC, Grogan G J Biol Chem. 2009 Oct 9;284(41):28467-75. Epub 2009 Aug 19. PMID:19692330<ref>PMID:19692330</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_19692330}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 2wiy" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 19692330 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_19692330}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Large Structures]]
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2WIY is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Rhodococcus Rhodococcus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WIY OCA].
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==Reference==
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<ref group="xtra">PMID:19692330</ref><references group="xtra"/>
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[[Category: Rhodococcus]]
[[Category: Rhodococcus]]
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[[Category: Bruce, N C.]]
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[[Category: Bruce NC]]
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[[Category: Grogan, G.]]
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[[Category: Grogan G]]
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[[Category: Jackson, R.]]
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[[Category: Jackson R]]
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[[Category: Sabbadin, F.]]
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[[Category: Sabbadin F]]
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[[Category: Bioremediation]]
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[[Category: Cyt-p450]]
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[[Category: Electron transport]]
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[[Category: Heme]]
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[[Category: Rdx]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Sep 3 15:58:10 2009''
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Current revision

Cytochrome P450 XplA heme domain P21212

PDB ID 2wiy

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