2w8b

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{{Seed}}
 
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[[Image:2w8b.png|left|200px]]
 
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==Crystal structure of processed TolB in complex with Pal==
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The line below this paragraph, containing "STRUCTURE_2w8b", creates the "Structure Box" on the page.
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<StructureSection load='2w8b' size='340' side='right'caption='[[2w8b]], [[Resolution|resolution]] 1.86&Aring;' scene=''>
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2w8b]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2W8B OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2W8B FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.86&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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{{STRUCTURE_2w8b| PDB=2w8b | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2w8b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2w8b OCA], [https://pdbe.org/2w8b PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2w8b RCSB], [https://www.ebi.ac.uk/pdbsum/2w8b PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2w8b ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/TOLB_ECOLI TOLB_ECOLI] Involved in the TonB-independent uptake of group A colicins (colicins A, E1, E2, E3 and K). Necessary for the colicins to reach their respective targets after initial binding to the bacteria.[HAMAP-Rule:MF_00671]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/w8/2w8b_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2w8b ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The Tol system is a five-protein assembly parasitized by colicins and bacteriophages that helps stabilize the Gram-negative outer membrane (OM). We show that allosteric signalling through the six-bladed beta-propeller protein TolB is central to Tol function in Escherichia coli and that this is subverted by colicins such as ColE9 to initiate their OM translocation. Protein-protein interactions with the TolB beta-propeller govern two conformational states that are adopted by the distal N-terminal 12 residues of TolB that bind TolA in the inner membrane. ColE9 promotes disorder of this 'TolA box' and recruitment of TolA. In contrast to ColE9, binding of the OM lipoprotein Pal to the same site induces conformational changes that sequester the TolA box to the TolB surface in which it exhibits little or no TolA binding. Our data suggest that Pal is an OFF switch for the Tol assembly, whereas colicins promote an ON state even though mimicking Pal. Comparison of the TolB mechanism to that of vertebrate guanine nucleotide exchange factor RCC1 suggests that allosteric signalling may be more prevalent in beta-propeller proteins than currently realized.
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===CRYSTAL STRUCTURE OF PROCESSED TOLB IN COMPLEX WITH PAL===
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Allosteric beta-propeller signalling in TolB and its manipulation by translocating colicins.,Bonsor DA, Hecht O, Vankemmelbeke M, Sharma A, Krachler AM, Housden NG, Lilly KJ, James R, Moore GR, Kleanthous C EMBO J. 2009 Sep 16;28(18):2846-57. Epub 2009 Aug 20. PMID:19696740<ref>PMID:19696740</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 2w8b" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_19696740}}, adds the Publication Abstract to the page
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*[[Pal|Pal]]
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(as it appears on PubMed at http://www.pubmed.gov), where 19696740 is the PubMed ID number.
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*[[TolB|TolB]]
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== References ==
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{{ABSTRACT_PUBMED_19696740}}
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<references/>
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__TOC__
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==About this Structure==
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</StructureSection>
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2W8B is a 8 chains structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2W8B OCA].
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==Reference==
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<ref group="xtra">PMID:19696740</ref><references group="xtra"/>
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[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
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[[Category: Bonsor, D A.]]
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[[Category: Large Structures]]
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[[Category: Kleanthous, C.]]
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[[Category: Bonsor DA]]
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[[Category: Sharma, A.]]
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[[Category: Kleanthous C]]
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[[Category: Bacteriocin transport]]
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[[Category: Sharma A]]
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[[Category: Cell membrane]]
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[[Category: Cell outer membrane]]
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[[Category: Lipoprotein]]
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[[Category: Membrane]]
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[[Category: Outer membrane]]
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[[Category: Pal]]
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[[Category: Palmitate]]
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[[Category: Periplasm]]
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[[Category: Protein transport membrane protein complex]]
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[[Category: Protein transport/membrane protein complex]]
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[[Category: Tol]]
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[[Category: Tolb]]
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[[Category: Transport]]
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[[Category: Transport protein/lipoprotein]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Sep 3 16:02:07 2009''
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Current revision

Crystal structure of processed TolB in complex with Pal

PDB ID 2w8b

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