3cxk

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{{Seed}}
 
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[[Image:3cxk.png|left|200px]]
 
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==1.7 A Crystal structure of methionine-R-sulfoxide reductase from Burkholderia pseudomallei: crystallization in a microfluidic crystal card.==
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The line below this paragraph, containing "STRUCTURE_3cxk", creates the "Structure Box" on the page.
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<StructureSection load='3cxk' size='340' side='right'caption='[[3cxk]], [[Resolution|resolution]] 1.70&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[3cxk]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Burkholderia_pseudomallei_1710b Burkholderia pseudomallei 1710b]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CXK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3CXK FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.7&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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{{STRUCTURE_3cxk| PDB=3cxk | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3cxk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3cxk OCA], [https://pdbe.org/3cxk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3cxk RCSB], [https://www.ebi.ac.uk/pdbsum/3cxk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3cxk ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/MSRB_BURP1 MSRB_BURP1]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/cx/3cxk_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3cxk ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The Microcapillary Protein Crystallization System (MPCS) embodies a new semi-automated plug-based crystallization technology which enables nanolitre-volume screening of crystallization conditions in a plasticware format that allows crystals to be easily removed for traditional cryoprotection and X-ray diffraction data collection. Protein crystals grown in these plastic devices can be directly subjected to in situ X-ray diffraction studies. The MPCS integrates the formulation of crystallization cocktails with the preparation of the crystallization experiments. Within microfluidic Teflon tubing or the microfluidic circuitry of a plastic CrystalCard, approximately 10-20 nl volume droplets are generated, each representing a microbatch-style crystallization experiment with a different chemical composition. The entire protein sample is utilized in crystallization experiments. Sparse-matrix screening and chemical gradient screening can be combined in one comprehensive ;hybrid' crystallization trial. The technology lends itself well to optimization by high-granularity gradient screening using optimization reagents such as precipitation agents, ligands or cryoprotectants.
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===1.7 A Crystal structure of methionine-R-sulfoxide reductase from Burkholderia pseudomallei: crystallization in a microfluidic crystal card.===
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The plug-based nanovolume Microcapillary Protein Crystallization System (MPCS).,Gerdts CJ, Elliott M, Lovell S, Mixon MB, Napuli AJ, Staker BL, Nollert P, Stewart L Acta Crystallogr D Biol Crystallogr. 2008 Nov;64(Pt 11):1116-22. Epub 2008, Oct 18. PMID:19020349<ref>PMID:19020349</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_19020349}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 3cxk" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 19020349 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_19020349}}
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__TOC__
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</StructureSection>
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==About this Structure==
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3CXK is a 2 chains structure of sequences from [http://en.wikipedia.org/wiki/Burkholderia_pseudomallei_1710b Burkholderia pseudomallei 1710b]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CXK OCA].
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==Reference==
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<ref group="xtra">PMID:19020349</ref><references group="xtra"/>
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[[Category: Burkholderia pseudomallei 1710b]]
[[Category: Burkholderia pseudomallei 1710b]]
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[[Category: ATCG3D, Accelerated Technologies Center for Gene to 3D Structure.]]
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[[Category: Large Structures]]
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[[Category: Craigen, D.]]
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[[Category: Craigen D]]
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[[Category: Gerdts, C.]]
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[[Category: Gerdts C]]
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[[Category: Lovell, S.]]
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[[Category: Lovell S]]
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[[Category: SSGCID, Seattle Structural Genomics Center for Infectious Disease.]]
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[[Category: Staker B]]
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[[Category: Staker, B.]]
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[[Category: Stewart L]]
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[[Category: Stewart, L.]]
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[[Category: Accelerated technologies center for gene to 3d structure]]
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[[Category: Atcg3d]]
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[[Category: Burkholderia]]
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[[Category: Microfluidic labcard]]
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[[Category: Msrb]]
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[[Category: Oxidoreductase]]
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[[Category: Protein structure initiative]]
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[[Category: Psi-2]]
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[[Category: Seattle structural genomics center for infectious disease]]
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[[Category: Ssgcid]]
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[[Category: Structural genomic]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Sep 3 16:04:42 2009''
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Current revision

1.7 A Crystal structure of methionine-R-sulfoxide reductase from Burkholderia pseudomallei: crystallization in a microfluidic crystal card.

PDB ID 3cxk

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