2w43

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{{Seed}}
 
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[[Image:2w43.png|left|200px]]
 
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==Structure of L-haloacid dehalogenase from S. tokodaii==
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The line below this paragraph, containing "STRUCTURE_2w43", creates the "Structure Box" on the page.
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<StructureSection load='2w43' size='340' side='right'caption='[[2w43]], [[Resolution|resolution]] 1.66&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2w43]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Sulfurisphaera_tokodaii Sulfurisphaera tokodaii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2W43 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2W43 FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.66&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MES:2-(N-MORPHOLINO)-ETHANESULFONIC+ACID'>MES</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
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{{STRUCTURE_2w43| PDB=2w43 | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2w43 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2w43 OCA], [https://pdbe.org/2w43 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2w43 RCSB], [https://www.ebi.ac.uk/pdbsum/2w43 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2w43 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/Q96XE7_SULTO Q96XE7_SULTO]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/w4/2w43_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2w43 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Haloacid dehalogenases have potential applications in the pharmaceutical and fine chemical industry as well as in the remediation of contaminated land. The L: -2-haloacid dehalogenase from the thermophilic archaeon Sulfolobus tokodaii has been cloned and over-expressed in Escherichia coli and successfully purified to homogeneity. Here we report the structure of the recombinant dehalogenase solved by molecular replacement in two different crystal forms. The enzyme is a homodimer with each monomer being composed of a core-domain of a beta-sheet bundle surrounded by alpha-helices and an alpha-helical sub-domain. This fold is similar to previously solved mesophilic L: -haloacid dehalogenase structures. The monoclinic crystal form contains a putative inhibitor L: -lactate in the active site. The enzyme displays haloacid dehalogenase activity towards carboxylic acids with the halide attached at the C2 position with the highest activity towards chloropropionic acid. The enzyme is thermostable with maximum activity at 60 degrees C and a half-life of over 1 h at 70 degrees C. The enzyme is relatively stable to solvents with 25% activity lost when incubated for 1 h in 20% v/v DMSO.
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===STRUCTURE OF L-HALOACID DEHALOGENASE FROM S. TOKODAII===
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Biochemical and structural studies of a L: -haloacid dehalogenase from the thermophilic archaeon Sulfolobus tokodaii.,Rye CA, Isupov MN, Lebedev AA, Littlechild JA Extremophiles. 2008 Nov 29. PMID:19039518<ref>PMID:19039518</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 2w43" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_19039518}}, adds the Publication Abstract to the page
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*[[Dehalogenase 3D structures|Dehalogenase 3D structures]]
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(as it appears on PubMed at http://www.pubmed.gov), where 19039518 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_19039518}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Large Structures]]
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2W43 is a 2 chains structure of sequences from [http://en.wikipedia.org/wiki/Sulfolobus_tokodaii Sulfolobus tokodaii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2W43 OCA].
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[[Category: Sulfurisphaera tokodaii]]
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[[Category: Isupov MN]]
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==Reference==
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[[Category: Lebedev AA]]
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<ref group="xtra">PMID:19039518</ref><references group="xtra"/>
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[[Category: Littlechild JA]]
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[[Category: Diisopropyl-fluorophosphatase]]
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[[Category: Rye CA]]
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[[Category: Sulfolobus tokodaii]]
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[[Category: Isupov, M N.]]
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[[Category: Lebedev, A A.]]
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[[Category: Littlechild, J A.]]
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[[Category: Rye, C A.]]
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[[Category: Hydrolase]]
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[[Category: Metabolic process]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Sep 3 16:27:12 2009''
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Current revision

Structure of L-haloacid dehalogenase from S. tokodaii

PDB ID 2w43

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