2wiq

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{{Seed}}
 
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[[Image:2wiq.png|left|200px]]
 
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==Fluorescent protein KillerRed in the native state==
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The line below this paragraph, containing "STRUCTURE_2wiq", creates the "Structure Box" on the page.
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<StructureSection load='2wiq' size='340' side='right'caption='[[2wiq]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2wiq]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Anthomedusae_sp._DC-2005 Anthomedusae sp. DC-2005]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WIQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2WIQ FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CRQ:[2-(3-CARBAMOYL-1-IMINO-PROPYL)-4-(4-HYDROXY-BENZYLIDENE)-5-OXO-4,5-DIHYDRO-IMIDAZOL-1-YL]-ACETIC+ACID'>CRQ</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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{{STRUCTURE_2wiq| PDB=2wiq | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2wiq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wiq OCA], [https://pdbe.org/2wiq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2wiq RCSB], [https://www.ebi.ac.uk/pdbsum/2wiq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2wiq ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/Q2TCH5_9CNID Q2TCH5_9CNID]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/wi/2wiq_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2wiq ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The red fluorescent protein KillerRed, engineered from the hydrozoan chromoprotein anm2CP, has been reported to induce strong cytotoxicity through the chromophore assisted light inactivation (CALI) effect. Here, we present the X-ray structures of KillerRed in its native and bleached states. A long water-filled channel is revealed, connecting the methylene bridge of the chromophore to the solvent. This channel facilitates the transit of oxygen and of reactive oxygen species (ROS) formed by reaction with the excited chromophore. The functional roles of key mutations used to produce KillerRed are discussed, strong chromophore distortions in the bleached state are revealed, and mechanisms for ROS production and self protection are proposed. The presence of a partially mature, photo-resistant, green-emitting state is characterized, which accounts for enhanced CALI by "pre-bleached" KillerRed.
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===FLUORESCENT PROTEIN KILLERRED IN THE NATIVE STATE===
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Structural basis for the phototoxicity of the fluorescent protein KillerRed.,Carpentier P, Violot S, Blanchoin L, Bourgeois D FEBS Lett. 2009 Sep 3;583(17):2839-42. Epub 2009 Jul 30. PMID:19646983<ref>PMID:19646983</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_19646983}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 2wiq" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 19646983 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_19646983}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Anthomedusae sp. DC-2005]]
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2WIQ is a 2 chains structure of sequences from [http://en.wikipedia.org/wiki/Eukaryota Eukaryota]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WIQ OCA].
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[[Category: Large Structures]]
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[[Category: Blanchoin L]]
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==Reference==
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[[Category: Bourgeois D]]
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<ref group="xtra">PMID:19646983</ref><references group="xtra"/>
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[[Category: Carpentier P]]
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[[Category: Eukaryota]]
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[[Category: Violot S]]
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[[Category: Blanchoin, L.]]
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[[Category: Bourgeois, D.]]
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[[Category: Carpentier, P.]]
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[[Category: Violot, S.]]
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[[Category: Cali]]
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[[Category: Fluorescent protein]]
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[[Category: Reactive oxygen species]]
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[[Category: Ro]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Sep 9 08:27:43 2009''
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Current revision

Fluorescent protein KillerRed in the native state

PDB ID 2wiq

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