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| - | {{Seed}} | |
| - | [[Image:3id1.png|left|200px]] | |
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| - | <!--
| + | ==Crystal Structure of RseP PDZ1 domain== |
| - | The line below this paragraph, containing "STRUCTURE_3id1", creates the "Structure Box" on the page.
| + | <StructureSection load='3id1' size='340' side='right'caption='[[3id1]], [[Resolution|resolution]] 1.67Å' scene=''> |
| - | You may change the PDB parameter (which sets the PDB file loaded into the applet)
| + | == Structural highlights == |
| - | or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
| + | <table><tr><td colspan='2'>[[3id1]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ID1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3ID1 FirstGlance]. <br> |
| - | or leave the SCENE parameter empty for the default display.
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.67Å</td></tr> |
| - | -->
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3id1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3id1 OCA], [https://pdbe.org/3id1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3id1 RCSB], [https://www.ebi.ac.uk/pdbsum/3id1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3id1 ProSAT]</span></td></tr> |
| - | {{STRUCTURE_3id1| PDB=3id1 | SCENE= }}
| + | </table> |
| - | | + | == Function == |
| - | ===Crystal Structure of RseP PDZ1 domain===
| + | [https://www.uniprot.org/uniprot/RSEP_ECOLI RSEP_ECOLI] A site-2 regulated intramembrane protease (S2P) that cleaves the peptide bond between 'Ala-108' and 'Cys-109' in the transmembrane region of RseA. Part of a regulated intramembrane proteolysis (RIP) cascade. Acts on DegS-cleaved RseA to release the cytoplasmic domain of RseA, residue "Val-148" of RseA may be required for this. This provides the cell with sigma-E (RpoE) activity through the proteolysis of RseA. Can also cleave sequences in transmembrane regions of other proteins (such as LacY) as well as liberated signal peptides of beta-lactamase, OmpF, LivK, SecM, PhoA, LivJ, OmpC, Lpp and TorA, probably within the membrane.<ref>PMID:11750129</ref> <ref>PMID:12183368</ref> <ref>PMID:12183369</ref> <ref>PMID:15496982</ref> <ref>PMID:18268014</ref> <ref>PMID:21810987</ref> <ref>PMID:18945679</ref> |
| - | | + | == Evolutionary Conservation == |
| - | | + | [[Image:Consurf_key_small.gif|200px|right]] |
| - | <!-- | + | Check<jmol> |
| - | The line below this paragraph, {{ABSTRACT_PUBMED_19706448}}, adds the Publication Abstract to the page
| + | <jmolCheckbox> |
| - | (as it appears on PubMed at http://www.pubmed.gov), where 19706448 is the PubMed ID number.
| + | <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/id/3id1_consurf.spt"</scriptWhenChecked> |
| - | -->
| + | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> |
| - | {{ABSTRACT_PUBMED_19706448}}
| + | <text>to colour the structure by Evolutionary Conservation</text> |
| - | | + | </jmolCheckbox> |
| - | ==About this Structure== | + | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3id1 ConSurf]. |
| - | 3ID1 is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli_k-12 Escherichia coli k-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ID1 OCA].
| + | <div style="clear:both"></div> |
| - | | + | == References == |
| - | ==Reference== | + | <references/> |
| - | <ref group="xtra">PMID:19706448</ref><references group="xtra"/> | + | __TOC__ |
| - | [[Category: Escherichia coli k-12]] | + | </StructureSection> |
| - | [[Category: Feng, L.]] | + | [[Category: Escherichia coli K-12]] |
| - | [[Category: Li, X.]] | + | [[Category: Large Structures]] |
| - | [[Category: Shi, Y.]] | + | [[Category: Feng L]] |
| - | [[Category: Wang, B.]] | + | [[Category: Li X]] |
| - | [[Category: Wang, J.]] | + | [[Category: Shi Y]] |
| - | [[Category: Cell inner membrane]] | + | [[Category: Wang B]] |
| - | [[Category: Cell membrane]] | + | [[Category: Wang J]] |
| - | [[Category: Hydrolase]] | + | |
| - | [[Category: Membrane]] | + | |
| - | [[Category: Metal-binding]] | + | |
| - | [[Category: Metalloprotease]] | + | |
| - | [[Category: Protease]] | + | |
| - | [[Category: Transmembrane]] | + | |
| - | [[Category: Zinc]] | + | |
| - | | + | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Sep 9 08:31:41 2009''
| + | |
| Structural highlights
Function
RSEP_ECOLI A site-2 regulated intramembrane protease (S2P) that cleaves the peptide bond between 'Ala-108' and 'Cys-109' in the transmembrane region of RseA. Part of a regulated intramembrane proteolysis (RIP) cascade. Acts on DegS-cleaved RseA to release the cytoplasmic domain of RseA, residue "Val-148" of RseA may be required for this. This provides the cell with sigma-E (RpoE) activity through the proteolysis of RseA. Can also cleave sequences in transmembrane regions of other proteins (such as LacY) as well as liberated signal peptides of beta-lactamase, OmpF, LivK, SecM, PhoA, LivJ, OmpC, Lpp and TorA, probably within the membrane.[1] [2] [3] [4] [5] [6] [7]
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
References
- ↑ Kanehara K, Akiyama Y, Ito K. Characterization of the yaeL gene product and its S2P-protease motifs in Escherichia coli. Gene. 2001 Dec 27;281(1-2):71-9. PMID:11750129
- ↑ Kanehara K, Ito K, Akiyama Y. YaeL (EcfE) activates the sigma(E) pathway of stress response through a site-2 cleavage of anti-sigma(E), RseA. Genes Dev. 2002 Aug 15;16(16):2147-55. PMID:12183368 doi:http://dx.doi.org/10.1101/gad.1002302
- ↑ Alba BM, Leeds JA, Onufryk C, Lu CZ, Gross CA. DegS and YaeL participate sequentially in the cleavage of RseA to activate the sigma(E)-dependent extracytoplasmic stress response. Genes Dev. 2002 Aug 15;16(16):2156-68. PMID:12183369 doi:10.1101/gad.1008902
- ↑ Akiyama Y, Kanehara K, Ito K. RseP (YaeL), an Escherichia coli RIP protease, cleaves transmembrane sequences. EMBO J. 2004 Nov 10;23(22):4434-42. Epub 2004 Oct 21. PMID:15496982 doi:http://dx.doi.org/7600449
- ↑ Koide K, Ito K, Akiyama Y. Substrate recognition and binding by RseP, an Escherichia coli intramembrane protease. J Biol Chem. 2008 Apr 11;283(15):9562-70. doi: 10.1074/jbc.M709984200. Epub 2008 , Feb 11. PMID:18268014 doi:http://dx.doi.org/10.1074/jbc.M709984200
- ↑ Saito A, Hizukuri Y, Matsuo E, Chiba S, Mori H, Nishimura O, Ito K, Akiyama Y. Post-liberation cleavage of signal peptides is catalyzed by the site-2 protease (S2P) in bacteria. Proc Natl Acad Sci U S A. 2011 Aug 16;108(33):13740-5. doi:, 10.1073/pnas.1108376108. Epub 2011 Aug 2. PMID:21810987 doi:http://dx.doi.org/10.1073/pnas.1108376108
- ↑ Inaba K, Suzuki M, Maegawa K, Akiyama S, Ito K, Akiyama Y. A pair of circularly permutated PDZ domains control RseP, the S2P family intramembrane protease of Escherichia coli. J Biol Chem. 2008 Dec 12;283(50):35042-52. doi: 10.1074/jbc.M806603200. Epub 2008, Oct 22. PMID:18945679 doi:http://dx.doi.org/10.1074/jbc.M806603200
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