3h15

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{{Seed}}
 
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[[Image:3h15.png|left|200px]]
 
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==Crystal structure of replication initiation factor MCM10-ID bound to ssDNA==
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The line below this paragraph, containing "STRUCTURE_3h15", creates the "Structure Box" on the page.
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<StructureSection load='3h15' size='340' side='right'caption='[[3h15]], [[Resolution|resolution]] 2.72&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[3h15]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Xenopus_laevis Xenopus laevis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3H15 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3H15 FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.72&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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{{STRUCTURE_3h15| PDB=3h15 | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3h15 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3h15 OCA], [https://pdbe.org/3h15 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3h15 RCSB], [https://www.ebi.ac.uk/pdbsum/3h15 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3h15 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/MCM10_XENLA MCM10_XENLA] Acts as a replication initiation factor that brings together the MCM2-7 helicase and the DNA polymerase alpha/primase complex in order to initiate DNA replication. Additionally, plays a role in preventing DNA damage during replication (By similarity).<ref>PMID:11864598</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/h1/3h15_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3h15 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Mcm10 is an essential eukaryotic protein required for the initiation and elongation phases of chromosomal replication. Specifically, Mcm10 is required for the association of several replication proteins, including DNA polymerase alpha (pol alpha), with chromatin. We showed previously that the internal (ID) and C-terminal (CTD) domains of Mcm10 physically interact with both single-stranded (ss) DNA and the catalytic p180 subunit of pol alpha. However, the mechanism by which Mcm10 interacts with pol alpha on and off DNA is unclear. As a first step toward understanding the structural details for these critical intermolecular interactions, x-ray crystallography and NMR spectroscopy were used to map the binary interfaces between Mcm10-ID, ssDNA, and p180. The crystal structure of an Mcm10-ID*ssDNA complex confirmed and extended our previous evidence that ssDNA binds within the oligonucleotide/oligosaccharide binding-fold cleft of Mcm10-ID. We show using NMR chemical shift perturbation and fluorescence spectroscopy that p180 also binds to the OB-fold and that ssDNA and p180 compete for binding to this motif. In addition, we map a minimal Mcm10 binding site on p180 to a small region within the p180 N-terminal domain (residues 286-310). These findings, together with data for DNA and p180 binding to an Mcm10 construct that contains both the ID and CTD, provide the first mechanistic insight into how Mcm10 might use a handoff mechanism to load and stabilize pol alpha within the replication fork.
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===Crystal structure of replication initiation factor MCM10-ID bound to ssDNA===
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Physical interactions between Mcm10, DNA, and DNA polymerase alpha.,Warren EM, Huang H, Fanning E, Chazin WJ, Eichman BF J Biol Chem. 2009 Sep 4;284(36):24662-72. Epub 2009 Jul 16. PMID:19608746<ref>PMID:19608746</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_19608746}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 3h15" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 19608746 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_19608746}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Large Structures]]
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3H15 is a 2 chains structure of sequences from [http://en.wikipedia.org/wiki/Xenopus_laevis Xenopus laevis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3H15 OCA].
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==Reference==
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<ref group="xtra">PMID:19608746</ref><references group="xtra"/>
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[[Category: Xenopus laevis]]
[[Category: Xenopus laevis]]
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[[Category: Eichman, B F.]]
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[[Category: Eichman BF]]
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[[Category: Warren, E M.]]
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[[Category: Warren EM]]
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[[Category: Ccch]]
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[[Category: Dna replication]]
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[[Category: Dna-binding]]
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[[Category: Metal-binding]]
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[[Category: Nucleus]]
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[[Category: Ob-fold]]
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[[Category: Replication/dna complex]]
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[[Category: Ssdna]]
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[[Category: Zinc]]
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[[Category: Zinc finger]]
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[[Category: Zinc-finger]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Sep 16 08:36:30 2009''
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Current revision

Crystal structure of replication initiation factor MCM10-ID bound to ssDNA

PDB ID 3h15

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