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1c58

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(New page: 200px<br /><applet load="1c58" size="450" color="white" frame="true" align="right" spinBox="true" caption="1c58, resolution 0.99&Aring;" /> '''CRYSTAL STRUCTURE OF...)
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[[Image:1c58.gif|left|200px]]<br /><applet load="1c58" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1c58, resolution 0.99&Aring;" />
 
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'''CRYSTAL STRUCTURE OF CYCLOAMYLOSE 26'''<br />
 
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==Overview==
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==CRYSTAL STRUCTURE OF CYCLOAMYLOSE 26==
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The amylose fraction of starch occurs in double-helical A- and B-amyloses, and the single-helical V-amylose. The latter contains a channel-like, central cavity that is able to include molecules, "iodine's blue" being, the best-known representative. Molecular models of these amylose forms, have been deduced by solid state 13C cross-polarization/magic angle, spinning NMR and by x-ray fiber and electron diffraction combined with, computer-aided modeling. They remain uncertain, however, as no structure, at atomic resolution is available. We report here the crystal structure of, a hydrated cycloamylose containing 26 glucose residues, (cyclomaltohexaicosaose, CA26), which has been determined by, real/reciprocal space recycling starting from randomly positioned atoms or, from an oriented diglucose fragment. This structure provides conclusive, evidence for the structure of V-amylose, as the macrocycle of CA26 is, folded into two short left-handed V-amylose helices in antiparallel, arrangement and related by twofold rotational pseudosymmetry. In the, V-helices, all glucose residues are in syn orientation, forming systematic, interglucose O(3)n...O(2)(n+l) and O(6)n...O(2)(n+6)/O(3)(n+6) hydrogen, bonds; the central cavities of the V-helices are filled by disordered, water molecules. The folding of the CA26 macrocycle is characterized by, typical "band-flips" in which diametrically opposed glucose residues are, in anti rather than in the common syn orientation, this conformation being, stabilized by interglucose three-center hydrogen bonds with O(3)n as donor, and O(5)(n+l), O(6)(n+l) as acceptors. The structure of CA26 permitted, construction of an idealized V-amylose helix, and the band-flip motif, explains why V-amylose crystallizes readily and may be packed tightly in, seeds.
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<StructureSection load='1c58' size='340' side='right'caption='[[1c58]], [[Resolution|resolution]] 0.99&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1C58 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1C58 FirstGlance]. <br>
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1C58 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1C58 OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-RAY DIFFRACTION, [[Resolution|Resolution]] 0.99&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1c58 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1c58 OCA], [https://pdbe.org/1c58 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1c58 RCSB], [https://www.ebi.ac.uk/pdbsum/1c58 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1c58 ProSAT]</span></td></tr>
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V-Amylose at atomic resolution: X-ray structure of a cycloamylose with 26 glucose residues (cyclomaltohexaicosaose)., Gessler K, Uson I, Takaha T, Krauss N, Smith SM, Okada S, Sheldrick GM, Saenger W, Proc Natl Acad Sci U S A. 1999 Apr 13;96(8):4246-51. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10200247 10200247]
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</table>
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[[Category: Protein complex]]
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__TOC__
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[[Category: Gessler, K.]]
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</StructureSection>
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[[Category: Nimz, O.]]
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[[Category: Large Structures]]
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[[Category: Saenger, W.]]
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[[Category: Gessler, K]]
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[[Category: cycloamylose]]
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[[Category: Nimz, O]]
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[[Category: Saenger, W]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sun Nov 25 01:07:58 2007''
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[[Category: Carbohydrate]]
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[[Category: Cycloamylose]]

Current revision

CRYSTAL STRUCTURE OF CYCLOAMYLOSE 26

PDB ID 1c58

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