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2rpv

From Proteopedia

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{{Seed}}
 
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[[Image:2rpv.jpg|left|200px]]
 
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<!--
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==Solution Structure of GB1 with LBT probe==
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The line below this paragraph, containing "STRUCTURE_2rpv", creates the "Structure Box" on the page.
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<StructureSection load='2rpv' size='340' side='right'caption='[[2rpv]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2rpv]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Strsg Strsg]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RPV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2RPV FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=LA:LANTHANUM+(III)+ION'>LA</scene></td></tr>
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-->
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2rpv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2rpv OCA], [https://pdbe.org/2rpv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2rpv RCSB], [https://www.ebi.ac.uk/pdbsum/2rpv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2rpv ProSAT]</span></td></tr>
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{{STRUCTURE_2rpv| PDB=2rpv | SCENE= }}
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</table>
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== Function ==
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[[https://www.uniprot.org/uniprot/SPG1_STRSG SPG1_STRSG]] Binds to the constant Fc region of IgG with high affinity.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/rp/2rpv_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2rpv ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Paramagnetic lanthanide ions fixed in a protein frame induce several paramagnetic effects such as pseudo-contact shifts and residual dipolar couplings. These effects provide long-range distance and angular information for proteins and, therefore, are valuable in protein structural analysis. However, until recently this approach had been restricted to metal-binding proteins, but now it has become applicable to non-metalloproteins through the use of a lanthanide-binding tag. Here we report a lanthanide-binding peptide tag anchored via two points to the target proteins. Compared to conventional single-point attached tags, the two-point linked tag provides two to threefold stronger anisotropic effects. Though there is slight residual mobility of the lanthanide-binding tag, the present tag provides a higher anisotropic paramagnetic effect.
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===Solution Structure of GB1 with LBT probe===
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Two-point anchoring of a lanthanide-binding peptide to a target protein enhances the paramagnetic anisotropic effect.,Saio T, Ogura K, Yokochi M, Kobashigawa Y, Inagaki F J Biomol NMR. 2009 Jul;44(3):157-66. Epub 2009 May 26. PMID:19468839<ref>PMID:19468839</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 2rpv" style="background-color:#fffaf0;"></div>
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<!--
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_19468839}}, adds the Publication Abstract to the page
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*[[Protein G|Protein G]]
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(as it appears on PubMed at http://www.pubmed.gov), where 19468839 is the PubMed ID number.
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== References ==
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-->
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<references/>
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{{ABSTRACT_PUBMED_19468839}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Large Structures]]
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2RPV is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Bacteria Bacteria]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RPV OCA].
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[[Category: Strsg]]
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[[Category: Inagaki, F]]
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==Reference==
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[[Category: Kobashigawa, Y]]
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<ref group="xtra">PMID:19468839</ref><references group="xtra"/>
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[[Category: Ogura, K]]
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[[Category: Bacteria]]
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[[Category: Saio, T]]
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[[Category: Inagaki, F.]]
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[[Category: Yokochi, M]]
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[[Category: Kobashigawa, Y.]]
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[[Category: Ogura, K.]]
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[[Category: Saio, T.]]
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[[Category: Yokochi, M.]]
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[[Category: Cell wall]]
[[Category: Cell wall]]
[[Category: Gb1]]
[[Category: Gb1]]
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[[Category: Peptidoglycan-anchor]]
[[Category: Peptidoglycan-anchor]]
[[Category: Secreted]]
[[Category: Secreted]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Sep 16 08:59:43 2009''
 

Current revision

Solution Structure of GB1 with LBT probe

PDB ID 2rpv

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