1kgz

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(New page: 200px<br /><applet load="1kgz" size="450" color="white" frame="true" align="right" spinBox="true" caption="1kgz, resolution 2.4&Aring;" /> '''Crystal Structure Ana...)
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[[Image:1kgz.jpg|left|200px]]<br /><applet load="1kgz" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1kgz, resolution 2.4&Aring;" />
 
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'''Crystal Structure Analysis of the Anthranilate Phosphoribosyltransferase from Erwinia carotovora (current name, Pectobacterium carotovorum)'''<br />
 
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==Overview==
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==Crystal Structure Analysis of the Anthranilate Phosphoribosyltransferase from Erwinia carotovora (current name, Pectobacterium carotovorum)==
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The structure of anthranilate phosphoribosyltransferase from the, enterobacterium Pectobacterium carotovorum has been solved at 2.4 A in, complex with Mn(2+)-pyrophosphate, and at 1.9 A without ligands. The, enzyme structure has a novel phosphoribosyltransferase (PRT) fold and, displays close homology to the structures of pyrimidine nucleoside, phosphorylases. The enzyme is a homodimer with a monomer of 345 residues., Each monomer consists of two subdomains, alpha and alpha/beta, which form, a cleft containing the active site. The nature of the active site is, inferred from the trapped MnPPi complex and detailed knowledge of the, active sites of nucleoside phosphorylases. With the anthranilate (An)PRT, structure solved, the structures of all the enzymes required for, tryptophan biosynthesis are now known.
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<StructureSection load='1kgz' size='340' side='right'caption='[[1kgz]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1kgz]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Pectobacterium_carotovorum Pectobacterium carotovorum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KGZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1KGZ FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HG:MERCURY+(II)+ION'>HG</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=PRP:ALPHA-PHOSPHORIBOSYLPYROPHOSPHORIC+ACID'>PRP</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1kgz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1kgz OCA], [https://pdbe.org/1kgz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1kgz RCSB], [https://www.ebi.ac.uk/pdbsum/1kgz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1kgz ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/Q8VP84_PECCA Q8VP84_PECCA] Catalyzes the transfer of the phosphoribosyl group of 5-phosphorylribose-1-pyrophosphate (PRPP) to anthranilate to yield N-(5'-phosphoribosyl)-anthranilate (PRA).[HAMAP-Rule:MF_00211][SAAS:SAAS00083122]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/kg/1kgz_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1kgz ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1KGZ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Pectobacterium_carotovorum Pectobacterium carotovorum] with HG, MN and PRP as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Anthranilate_phosphoribosyltransferase Anthranilate phosphoribosyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.2.18 2.4.2.18] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1KGZ OCA].
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*[[Phosphoribosyltransferase 3D structures|Phosphoribosyltransferase 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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The crystal structure of anthranilate phosphoribosyltransferase from the enterobacterium Pectobacterium carotovorum., Kim C, Xuong NH, Edwards S, Madhusudan, Yee MC, Spraggon G, Mills SE, FEBS Lett. 2002 Jul 17;523(1-3):239-46. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12123839 12123839]
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[[Category: Large Structures]]
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[[Category: Anthranilate phosphoribosyltransferase]]
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[[Category: Pectobacterium carotovorum]]
[[Category: Pectobacterium carotovorum]]
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[[Category: Single protein]]
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[[Category: Edwards S]]
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[[Category: Edwards, S.]]
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[[Category: Kim C]]
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[[Category: Kim, C.]]
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[[Category: Madhusudan]]
[[Category: Madhusudan]]
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[[Category: Mills, S.E.]]
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[[Category: Mills SE]]
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[[Category: Spraggon, G.]]
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[[Category: Spraggon G]]
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[[Category: Xuong, N.H.]]
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[[Category: Xuong N-H]]
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[[Category: Yee, M.C.]]
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[[Category: Yee M-C]]
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[[Category: HG]]
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[[Category: MN]]
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[[Category: PRP]]
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[[Category: nucleotide binding fold]]
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[[Category: type 3 prt fold]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sun Nov 25 01:20:54 2007''
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Current revision

Crystal Structure Analysis of the Anthranilate Phosphoribosyltransferase from Erwinia carotovora (current name, Pectobacterium carotovorum)

PDB ID 1kgz

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