3hhf

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{{Seed}}
 
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[[Image:3hhf.png|left|200px]]
 
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==Structure of CrgA regulatory domain, a LysR-type transcriptional regulator from Neisseria meningitidis.==
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The line below this paragraph, containing "STRUCTURE_3hhf", creates the "Structure Box" on the page.
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<StructureSection load='3hhf' size='340' side='right'caption='[[3hhf]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[3hhf]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Neisseria_meningitidis_serogroup_B Neisseria meningitidis serogroup B]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3HHF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3HHF FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
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{{STRUCTURE_3hhf| PDB=3hhf | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3hhf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3hhf OCA], [https://pdbe.org/3hhf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3hhf RCSB], [https://www.ebi.ac.uk/pdbsum/3hhf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3hhf ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/CRGA_NEIMB CRGA_NEIMB] Regulatory protein that activates transcription of mdaB, encoding a NADPH:quinone oxidoreductase, and represses its own transcription (PubMed:15866928). Under the same experimental conditions, no regulation of transcription of pilus and capsule genes is detected (PubMed:15866928).<ref>PMID:15866928</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/hh/3hhf_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3hhf ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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LysR-type transcriptional regulators (LTTRs) form the largest family of bacterial regulators acting as both auto-repressors and activators of target promoters, controlling operons involved in a wide variety of cellular processes. The LTTR, CrgA, from the human pathogen Neisseria meningitidis, is upregulated during bacterial-host cell contact. Here, we report the crystal structures of both regulatory domain and full-length CrgA, the first of a novel subclass of LTTRs that form octameric rings. Non-denaturing mass spectrometry analysis and analytical ultracentrifugation established that the octameric form of CrgA is the predominant species in solution in both the presence and absence of an oligonucleotide encompassing the CrgA-binding sequence. Furthermore, analysis of the isolated CrgA-DNA complex by mass spectrometry showed stabilization of a double octamer species upon DNA binding. Based on the observed structure and the mass spectrometry findings, a model is proposed in which a hexadecameric array of two CrgA oligomers binds to its DNA target site.
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===Structure of CrgA regulatory domain, a LysR-type transcriptional regulator from Neisseria meningitidis.===
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The structure of CrgA from Neisseria meningitidis reveals a new octameric assembly state for LysR transcriptional regulators.,Sainsbury S, Lane LA, Ren J, Gilbert RJ, Saunders NJ, Robinson CV, Stuart DI, Owens RJ Nucleic Acids Res. 2009 Aug;37(14):4545-58. Epub 2009 May 27. PMID:19474343<ref>PMID:19474343</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 3hhf" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_19474343}}, adds the Publication Abstract to the page
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*[[Transcriptional activator 3D structures|Transcriptional activator 3D structures]]
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(as it appears on PubMed at http://www.pubmed.gov), where 19474343 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_19474343}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Large Structures]]
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3HHF is a 2 chains structure of sequences from [http://en.wikipedia.org/wiki/Neisseria_meningitidis_serogroup_b Neisseria meningitidis serogroup b]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3HHF OCA].
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[[Category: Neisseria meningitidis serogroup B]]
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[[Category: Owens RJ]]
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==Reference==
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[[Category: Ren J]]
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<ref group="xtra">PMID:19474343</ref><references group="xtra"/>
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[[Category: Sainsbury S]]
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[[Category: Neisseria meningitidis serogroup b]]
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[[Category: Stuart DI]]
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[[Category: OPPF, Oxford Protein Production Facility.]]
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[[Category: Owens, R J.]]
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[[Category: Ren, J.]]
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[[Category: Sainsbury, S.]]
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[[Category: Stuart, D I.]]
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[[Category: Dna-binding]]
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[[Category: Lysr]]
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[[Category: Neisseria meningitidi]]
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[[Category: Oppf]]
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[[Category: Oxford protein production facility]]
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[[Category: Structural genomic]]
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[[Category: Transcription]]
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[[Category: Transcription factor]]
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[[Category: Transcription regulation]]
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[[Category: Transcription regulator]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Sep 21 21:00:50 2009''
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Current revision

Structure of CrgA regulatory domain, a LysR-type transcriptional regulator from Neisseria meningitidis.

PDB ID 3hhf

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