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1cgs

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(New page: 200px<br /><applet load="1cgs" size="450" color="white" frame="true" align="right" spinBox="true" caption="1cgs, resolution 2.6&Aring;" /> '''LOCAL AND TRANSMITTED...)
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[[Image:1cgs.gif|left|200px]]<br /><applet load="1cgs" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1cgs, resolution 2.6&Aring;" />
 
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'''LOCAL AND TRANSMITTED CONFORMATIONAL CHANGES ON COMPLEXATION OF AN ANTI-SWEETENER FAB'''<br />
 
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==Overview==
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==LOCAL AND TRANSMITTED CONFORMATIONAL CHANGES ON COMPLEXATION OF AN ANTI-SWEETENER FAB==
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Crystal structures of an Fab (NC6.8) from a murine IgG2b(kappa) antibody, and its complex with a sweet-tasting, N-,N'-,N"-trisubstituted guanidine, compound (NC174) have been determined by X-ray analysis. Both crystal, forms are produced by a microseeding technique in polyethylene glycol, (PEG) 8000 but the habits and space groups are very different. The native, protein crystallizes as plates in the monoclinic space group C2 and the, complex crystallizes as prisms in the orthorhombic space group P2(1)2(1)2., The structures were solved by molecular replacement methods, with the Fab, fragments from the 4-4-20, HyHel-5 and BV04-01 antibodies as starting, models. On binding of the ligand, N-(p-cyanophenyl)-N'-(diphenylmethyl)-N"-(carboxymethyl)g uan idine, the, protein exhibits significant local conformational changes in the active, site, particularly in the third complementarity-determining region (CDR3), of the heavy chain. The ligand enters the small crevice by end-on, insertion with the cyanophenyl group in the lead and the diphenyl rings, partially protruding from the entrance. No strict pi-pi stacking, interactions are observed. However, tyrosine L32 (CDR1), tyrosine L96, (CDR3) and tryptophan H33 (CDR1) help immobilize the cyanophenyl ring and, guanido group, and tyrosine H96 moves about 4.5 A to lie between the rings, of the diphenyl group. The positive charge on the guanido group is, compensated by glutamic acid H50 (CDR2) while the negative charge on, acetic acid is neutralized by arginine H56 (CDR2) and by hydrogen bonding, with asparagine H58 (CDR2). Water molecules participate in the binding, process by hydrogen bonding with the cyano and guanido groups. The, mechanism of binding is a clear example of induced fit. Like hemoglobin, the NC6.8 Fab can be classified as an allosteric protein, since its, overall structure is altered by the binding of a small ligand. In crystals, of the native Fab the elbow bend angle is 184 degrees while in crystals of, the complex the elbow angle is 153 degrees. There is also a reciprocal, push-pull type of change where the heavy chain is flexed and the light, chain is extended. The tail of the heavy chain, which would be connected, to the Fc in an intact antibody, is displaced 19 A relative to its, position in the unliganded Fab. Within the limited series of sweetener-Fab, complexes we have thus far examined, only the NC174 hapten has produced, such results.(ABSTRACT TRUNCATED AT 400 WORDS)
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<StructureSection load='1cgs' size='340' side='right'caption='[[1cgs]], [[Resolution|resolution]] 2.60&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1cgs]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CGS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1CGS FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.6&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1cgs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1cgs OCA], [https://pdbe.org/1cgs PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1cgs RCSB], [https://www.ebi.ac.uk/pdbsum/1cgs PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1cgs ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/A2P1G9_MOUSE A2P1G9_MOUSE]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/cg/1cgs_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1cgs ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1CGS is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1CGS OCA].
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*[[Antibody 3D structures|Antibody 3D structures]]
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*[[Sandbox 20009|Sandbox 20009]]
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==Reference==
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*[[3D structures of non-human antibody|3D structures of non-human antibody]]
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Local and transmitted conformational changes on complexation of an anti-sweetener Fab., Guddat LW, Shan L, Anchin JM, Linthicum DS, Edmundson AB, J Mol Biol. 1994 Feb 11;236(1):247-74. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=7893280 7893280]
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__TOC__
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[[Category: Protein complex]]
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</StructureSection>
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[[Category: Edmundson, A.B.]]
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[[Category: Large Structures]]
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[[Category: Guddat, L.W.]]
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[[Category: Mus musculus]]
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[[Category: Shan, L.]]
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[[Category: Edmundson AB]]
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[[Category: immunoglobulin]]
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[[Category: Guddat LW]]
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[[Category: Shan L]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sun Nov 25 01:31:05 2007''
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Current revision

LOCAL AND TRANSMITTED CONFORMATIONAL CHANGES ON COMPLEXATION OF AN ANTI-SWEETENER FAB

PDB ID 1cgs

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