1ovs

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(New page: 200px<br /><applet load="1ovs" size="450" color="white" frame="true" align="right" spinBox="true" caption="1ovs, resolution 1.75&Aring;" /> '''LecB (PA-LII) in com...)
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[[Image:1ovs.jpg|left|200px]]<br /><applet load="1ovs" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1ovs, resolution 1.75&Aring;" />
 
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'''LecB (PA-LII) in complex with core trimannoside'''<br />
 
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==Overview==
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==LecB (PA-LII) in complex with core trimannoside==
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The crystal structure of Pseudomonas aeruginosa fucose-specific lectin, LecB was determined in its metal-bound and metal-free state as well as in, complex with fucose, mannose and fructopyranose. All three monosaccharides, bind isosterically via direct interactions with two calcium ions as well, as direct hydrogen bonds with several side-chains. The higher affinity for, fucose is explained by the details of the binding site around C6 and O1 of, fucose. In the mannose and fructose complexes, a carboxylate oxygen atom, and one or two hydroxyl groups are partly shielded from solvent upon sugar, binding, preventing them from completely fulfilling their hydrogen bonding, potential. In the fucose complex, no such defects are observed. Instead, C6 makes favourable interactions with a small hydrophobic patch. Upon, demetallization, the C terminus as well as the otherwise rigid, metal-binding loop become more mobile and adopt multiple conformations.
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<StructureSection load='1ovs' size='340' side='right'caption='[[1ovs]], [[Resolution|resolution]] 1.75&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1ovs]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OVS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1OVS FirstGlance]. <br>
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1OVS is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa] with CA as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1OVS OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.75&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=PRD_900112:3alpha-alpha-mannobiose'>PRD_900112</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ovs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ovs OCA], [https://pdbe.org/1ovs PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ovs RCSB], [https://www.ebi.ac.uk/pdbsum/1ovs PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ovs ProSAT]</span></td></tr>
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Structural basis of carbohydrate recognition by the lectin LecB from Pseudomonas aeruginosa., Loris R, Tielker D, Jaeger KE, Wyns L, J Mol Biol. 2003 Aug 22;331(4):861-70. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12909014 12909014]
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/Q9HYN5_PSEAE Q9HYN5_PSEAE]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ov/1ovs_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ovs ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Pseudomonas aeruginosa]]
[[Category: Pseudomonas aeruginosa]]
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[[Category: Single protein]]
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[[Category: Jaeger K-E]]
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[[Category: Jaeger, K.E.]]
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[[Category: Loris R]]
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[[Category: Loris, R.]]
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[[Category: Tielker D]]
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[[Category: Tielker, D.]]
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[[Category: Wyns L]]
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[[Category: Wyns, L.]]
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[[Category: CA]]
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[[Category: carbohydrate]]
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[[Category: lectin]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sun Nov 25 01:31:48 2007''
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Current revision

LecB (PA-LII) in complex with core trimannoside

PDB ID 1ovs

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