1kq1
From Proteopedia
(New page: 200px<br /><applet load="1kq1" size="450" color="white" frame="true" align="right" spinBox="true" caption="1kq1, resolution 1.55Å" /> '''1.55 A Crystal struc...) |
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| - | [[Image:1kq1.gif|left|200px]]<br /><applet load="1kq1" size=" | + | [[Image:1kq1.gif|left|200px]]<br /><applet load="1kq1" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1kq1, resolution 1.55Å" /> | caption="1kq1, resolution 1.55Å" /> | ||
'''1.55 A Crystal structure of the pleiotropic translational regulator, Hfq'''<br /> | '''1.55 A Crystal structure of the pleiotropic translational regulator, Hfq'''<br /> | ||
==Overview== | ==Overview== | ||
| - | In prokaryotes, Hfq regulates translation by modulating the structure of | + | In prokaryotes, Hfq regulates translation by modulating the structure of numerous RNA molecules by binding preferentially to A/U-rich sequences. To elucidate the mechanisms of target recognition and translation regulation by Hfq, we determined the crystal structures of the Staphylococcus aureus Hfq and an Hfq-RNA complex to 1.55 and 2.71 A resolution, respectively. The structures reveal that Hfq possesses the Sm-fold previously observed only in eukaryotes and archaea. However, unlike these heptameric Sm proteins, Hfq forms a homo-hexameric ring. The Hfq-RNA structure reveals that the single-stranded hepta-oligoribonucleotide binds in a circular conformation around a central basic cleft, whereby Tyr42 residues from adjacent subunits stack with six of the bases, and Gln8, outside the Sm motif, provides key protein-base contacts. Such binding suggests a mechanism for Hfq function. |
==About this Structure== | ==About this Structure== | ||
| - | 1KQ1 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus] with ACY as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | + | 1KQ1 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus] with <scene name='pdbligand=ACY:'>ACY</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KQ1 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Staphylococcus aureus]] | [[Category: Staphylococcus aureus]] | ||
| - | [[Category: Brennan, R | + | [[Category: Brennan, R G.]] |
[[Category: Moller, T.]] | [[Category: Moller, T.]] | ||
| - | [[Category: Pearson, R | + | [[Category: Pearson, R F.]] |
| - | [[Category: Schumacher, M | + | [[Category: Schumacher, M A.]] |
[[Category: Valentin-Hansen, P.]] | [[Category: Valentin-Hansen, P.]] | ||
[[Category: ACY]] | [[Category: ACY]] | ||
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[[Category: translational regulator]] | [[Category: translational regulator]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:36:45 2008'' |
Revision as of 11:36, 21 February 2008
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1.55 A Crystal structure of the pleiotropic translational regulator, Hfq
Overview
In prokaryotes, Hfq regulates translation by modulating the structure of numerous RNA molecules by binding preferentially to A/U-rich sequences. To elucidate the mechanisms of target recognition and translation regulation by Hfq, we determined the crystal structures of the Staphylococcus aureus Hfq and an Hfq-RNA complex to 1.55 and 2.71 A resolution, respectively. The structures reveal that Hfq possesses the Sm-fold previously observed only in eukaryotes and archaea. However, unlike these heptameric Sm proteins, Hfq forms a homo-hexameric ring. The Hfq-RNA structure reveals that the single-stranded hepta-oligoribonucleotide binds in a circular conformation around a central basic cleft, whereby Tyr42 residues from adjacent subunits stack with six of the bases, and Gln8, outside the Sm motif, provides key protein-base contacts. Such binding suggests a mechanism for Hfq function.
About this Structure
1KQ1 is a Single protein structure of sequence from Staphylococcus aureus with as ligand. Full crystallographic information is available from OCA.
Reference
Structures of the pleiotropic translational regulator Hfq and an Hfq-RNA complex: a bacterial Sm-like protein., Schumacher MA, Pearson RF, Moller T, Valentin-Hansen P, Brennan RG, EMBO J. 2002 Jul 1;21(13):3546-56. PMID:12093755
Page seeded by OCA on Thu Feb 21 13:36:45 2008
