1kq3
From Proteopedia
(New page: 200px<br /><applet load="1kq3" size="450" color="white" frame="true" align="right" spinBox="true" caption="1kq3, resolution 1.50Å" /> '''Crystal Structure of...) |
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| - | [[Image:1kq3.gif|left|200px]]<br /><applet load="1kq3" size=" | + | [[Image:1kq3.gif|left|200px]]<br /><applet load="1kq3" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1kq3, resolution 1.50Å" /> | caption="1kq3, resolution 1.50Å" /> | ||
'''Crystal Structure of Glycerol Dehydrogenase (TM0423) from Thermotoga maritima at 1.5 A Resolution'''<br /> | '''Crystal Structure of Glycerol Dehydrogenase (TM0423) from Thermotoga maritima at 1.5 A Resolution'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Structural genomics is emerging as a principal approach to define protein | + | Structural genomics is emerging as a principal approach to define protein structure-function relationships. To apply this approach on a genomic scale, novel methods and technologies must be developed to determine large numbers of structures. We describe the design and implementation of a high-throughput structural genomics pipeline and its application to the proteome of the thermophilic bacterium Thermotoga maritima. By using this pipeline, we successfully cloned and attempted expression of 1,376 of the predicted 1,877 genes (73%) and have identified crystallization conditions for 432 proteins, comprising 23% of the T. maritima proteome. Representative structures from TM0423 glycerol dehydrogenase and TM0449 thymidylate synthase-complementing protein are presented as examples of final outputs from the pipeline. |
==About this Structure== | ==About this Structure== | ||
| - | 1KQ3 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Thermotoga_maritima Thermotoga maritima] with ZN, CL and TRS as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http:// | + | 1KQ3 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Thermotoga_maritima Thermotoga maritima] with <scene name='pdbligand=ZN:'>ZN</scene>, <scene name='pdbligand=CL:'>CL</scene> and <scene name='pdbligand=TRS:'>TRS</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KQ3 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Thermotoga maritima]] | [[Category: Thermotoga maritima]] | ||
| - | [[Category: JCSG, Joint | + | [[Category: JCSG, Joint Center for Structural Genomics.]] |
| - | [[Category: Miller, M | + | [[Category: Miller, M D.]] |
| - | [[Category: Wilson, I | + | [[Category: Wilson, I A]] |
[[Category: CL]] | [[Category: CL]] | ||
[[Category: TRS]] | [[Category: TRS]] | ||
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[[Category: zinc]] | [[Category: zinc]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:36:44 2008'' |
Revision as of 11:36, 21 February 2008
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Crystal Structure of Glycerol Dehydrogenase (TM0423) from Thermotoga maritima at 1.5 A Resolution
Overview
Structural genomics is emerging as a principal approach to define protein structure-function relationships. To apply this approach on a genomic scale, novel methods and technologies must be developed to determine large numbers of structures. We describe the design and implementation of a high-throughput structural genomics pipeline and its application to the proteome of the thermophilic bacterium Thermotoga maritima. By using this pipeline, we successfully cloned and attempted expression of 1,376 of the predicted 1,877 genes (73%) and have identified crystallization conditions for 432 proteins, comprising 23% of the T. maritima proteome. Representative structures from TM0423 glycerol dehydrogenase and TM0449 thymidylate synthase-complementing protein are presented as examples of final outputs from the pipeline.
About this Structure
1KQ3 is a Single protein structure of sequence from Thermotoga maritima with , and as ligands. Full crystallographic information is available from OCA.
Reference
Structural genomics of the Thermotoga maritima proteome implemented in a high-throughput structure determination pipeline., Lesley SA, Kuhn P, Godzik A, Deacon AM, Mathews I, Kreusch A, Spraggon G, Klock HE, McMullan D, Shin T, Vincent J, Robb A, Brinen LS, Miller MD, McPhillips TM, Miller MA, Scheibe D, Canaves JM, Guda C, Jaroszewski L, Selby TL, Elsliger MA, Wooley J, Taylor SS, Hodgson KO, Wilson IA, Schultz PG, Stevens RC, Proc Natl Acad Sci U S A. 2002 Sep 3;99(18):11664-9. Epub 2002 Aug 22. PMID:12193646
Page seeded by OCA on Thu Feb 21 13:36:44 2008
