1guc
From Proteopedia
(New page: 200px<br /><applet load="1guc" size="450" color="white" frame="true" align="right" spinBox="true" caption="1guc" /> '''SOLUTION NMR STRUCTURE OF AN RNA WITH TANDEM...) |
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| - | [[Image:1guc.gif|left|200px]]<br /><applet load="1guc" size=" | + | [[Image:1guc.gif|left|200px]]<br /><applet load="1guc" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1guc" /> | caption="1guc" /> | ||
'''SOLUTION NMR STRUCTURE OF AN RNA WITH TANDEM, SYMMETRIC GU MISMATCHES, 30 STRUCTURES'''<br /> | '''SOLUTION NMR STRUCTURE OF AN RNA WITH TANDEM, SYMMETRIC GU MISMATCHES, 30 STRUCTURES'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The duplex (rGAGGUCUC)2 contains the motif [sequence: see text] which is | + | The duplex (rGAGGUCUC)2 contains the motif [sequence: see text] which is unusually stable compared with other symmetric tandem GU mismatches and occurs in the P5 helix of the group I intron of Tetrahymena thermophila. The three-dimensional solution structure of (rGAGGUCUC)2 was determined using two-dimensional NMR and a simulated annealing protocol. The structure is remarkably similar to the A-DNA crystal structure of (dGGGGTCCC)2 [Kneale, G., Brown, T., & Kennard, O. (1985) J. Mol. Biol. 186, 805-814] which contains the analogous motif [sequence: see text]. Incorporation of the [sequence: see text] motif has little effect on backbone torsion angles and helical parameters compared with standard A-form. The only significant departure from A-form is a slight overtwisting 5' of the G in the GU mismatch and a displacement of the mismatches toward the minor groove. Inspection of stacking patterns of this structure and comparison with symmetric tandem GT mismatches in A-DNA oligonucleotides from crystal structure data suggest that electrostatics are important in determining motif stability. |
==About this Structure== | ==About this Structure== | ||
| - | 1GUC is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http:// | + | 1GUC is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GUC OCA]. |
==Reference== | ==Reference== | ||
Investigation of the structural basis for thermodynamic stabilities of tandem GU mismatches: solution structure of (rGAGGUCUC)2 by two-dimensional NMR and simulated annealing., McDowell JA, Turner DH, Biochemistry. 1996 Nov 12;35(45):14077-89. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=8916893 8916893] | Investigation of the structural basis for thermodynamic stabilities of tandem GU mismatches: solution structure of (rGAGGUCUC)2 by two-dimensional NMR and simulated annealing., McDowell JA, Turner DH, Biochemistry. 1996 Nov 12;35(45):14077-89. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=8916893 8916893] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
| - | [[Category: Mcdowell, J | + | [[Category: Mcdowell, J A.]] |
| - | [[Category: Turner, D | + | [[Category: Turner, D H.]] |
[[Category: g:u mismatch]] | [[Category: g:u mismatch]] | ||
[[Category: ribonucleic acid]] | [[Category: ribonucleic acid]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:54:00 2008'' |
Revision as of 10:54, 21 February 2008
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SOLUTION NMR STRUCTURE OF AN RNA WITH TANDEM, SYMMETRIC GU MISMATCHES, 30 STRUCTURES
Overview
The duplex (rGAGGUCUC)2 contains the motif [sequence: see text] which is unusually stable compared with other symmetric tandem GU mismatches and occurs in the P5 helix of the group I intron of Tetrahymena thermophila. The three-dimensional solution structure of (rGAGGUCUC)2 was determined using two-dimensional NMR and a simulated annealing protocol. The structure is remarkably similar to the A-DNA crystal structure of (dGGGGTCCC)2 [Kneale, G., Brown, T., & Kennard, O. (1985) J. Mol. Biol. 186, 805-814] which contains the analogous motif [sequence: see text]. Incorporation of the [sequence: see text] motif has little effect on backbone torsion angles and helical parameters compared with standard A-form. The only significant departure from A-form is a slight overtwisting 5' of the G in the GU mismatch and a displacement of the mismatches toward the minor groove. Inspection of stacking patterns of this structure and comparison with symmetric tandem GT mismatches in A-DNA oligonucleotides from crystal structure data suggest that electrostatics are important in determining motif stability.
About this Structure
1GUC is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.
Reference
Investigation of the structural basis for thermodynamic stabilities of tandem GU mismatches: solution structure of (rGAGGUCUC)2 by two-dimensional NMR and simulated annealing., McDowell JA, Turner DH, Biochemistry. 1996 Nov 12;35(45):14077-89. PMID:8916893
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